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twenty-four defines a critical translational step in the Drosophila clock

Daily oscillations of gene expression underlie circadian behaviours in multicellular organisms1. While attention has been focused on transcriptional and posttranslational mechanisms1–3, other posttranscriptional modes have been less clearly delineated. Here we report mutants of a novel Drosophila ge...

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Autores principales: Lim, Chunghun, Lee, Jongbin, Choi, Changtaek, Kilman, Valerie L., Kim, Juwon, Park, Sung Mi, Jang, Sung Key, Allada, Ravi, Choe, Joonho
Formato: Texto
Lenguaje:English
Publicado: 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3073513/
https://www.ncbi.nlm.nih.gov/pubmed/21331043
http://dx.doi.org/10.1038/nature09728
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author Lim, Chunghun
Lee, Jongbin
Choi, Changtaek
Kilman, Valerie L.
Kim, Juwon
Park, Sung Mi
Jang, Sung Key
Allada, Ravi
Choe, Joonho
author_facet Lim, Chunghun
Lee, Jongbin
Choi, Changtaek
Kilman, Valerie L.
Kim, Juwon
Park, Sung Mi
Jang, Sung Key
Allada, Ravi
Choe, Joonho
author_sort Lim, Chunghun
collection PubMed
description Daily oscillations of gene expression underlie circadian behaviours in multicellular organisms1. While attention has been focused on transcriptional and posttranslational mechanisms1–3, other posttranscriptional modes have been less clearly delineated. Here we report mutants of a novel Drosophila gene twenty-four (tyf) that display weak behavioural rhythms. Weak rhythms are accompanied by dramatic reductions in the levels of the clock protein PERIOD (PER) as well as more modest effects on TIMELESS (TIM). Nonetheless, PER induction in pacemaker neurons can rescue tyf mutant rhythms. TYF associates with a 5′-cap binding complex, poly(A)-binding protein (PABP) as well as per and tim transcripts. Furthermore, TYF activates reporter expression when tethered to reporter mRNA even in vitro. Taken together, these data suggest that TYF potently activates PER translation in pacemaker neurons to sustain robust rhythms, revealing a novel and important role for translational control in the Drosophila circadian clock.
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spelling pubmed-30735132011-08-17 twenty-four defines a critical translational step in the Drosophila clock Lim, Chunghun Lee, Jongbin Choi, Changtaek Kilman, Valerie L. Kim, Juwon Park, Sung Mi Jang, Sung Key Allada, Ravi Choe, Joonho Nature Article Daily oscillations of gene expression underlie circadian behaviours in multicellular organisms1. While attention has been focused on transcriptional and posttranslational mechanisms1–3, other posttranscriptional modes have been less clearly delineated. Here we report mutants of a novel Drosophila gene twenty-four (tyf) that display weak behavioural rhythms. Weak rhythms are accompanied by dramatic reductions in the levels of the clock protein PERIOD (PER) as well as more modest effects on TIMELESS (TIM). Nonetheless, PER induction in pacemaker neurons can rescue tyf mutant rhythms. TYF associates with a 5′-cap binding complex, poly(A)-binding protein (PABP) as well as per and tim transcripts. Furthermore, TYF activates reporter expression when tethered to reporter mRNA even in vitro. Taken together, these data suggest that TYF potently activates PER translation in pacemaker neurons to sustain robust rhythms, revealing a novel and important role for translational control in the Drosophila circadian clock. 2011-02-17 /pmc/articles/PMC3073513/ /pubmed/21331043 http://dx.doi.org/10.1038/nature09728 Text en Users may view, print, copy, download and text and data- mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use: http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Lim, Chunghun
Lee, Jongbin
Choi, Changtaek
Kilman, Valerie L.
Kim, Juwon
Park, Sung Mi
Jang, Sung Key
Allada, Ravi
Choe, Joonho
twenty-four defines a critical translational step in the Drosophila clock
title twenty-four defines a critical translational step in the Drosophila clock
title_full twenty-four defines a critical translational step in the Drosophila clock
title_fullStr twenty-four defines a critical translational step in the Drosophila clock
title_full_unstemmed twenty-four defines a critical translational step in the Drosophila clock
title_short twenty-four defines a critical translational step in the Drosophila clock
title_sort twenty-four defines a critical translational step in the drosophila clock
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3073513/
https://www.ncbi.nlm.nih.gov/pubmed/21331043
http://dx.doi.org/10.1038/nature09728
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