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Microarray Analysis Uncovers a Role for Tip60 in Nervous System Function and General Metabolism
BACKGROUND: Tip60 is a key histone acetyltransferase (HAT) enzyme that plays a central role in diverse biological processes critical for general cell function; however, the chromatin-mediated cell-type specific developmental pathways that are dependent exclusively upon the HAT activity of Tip60 rema...
Autores principales: | , , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3073973/ https://www.ncbi.nlm.nih.gov/pubmed/21494552 http://dx.doi.org/10.1371/journal.pone.0018412 |
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author | Lorbeck, Meridith Pirooznia, Keerthy Sarthi, Jessica Zhu, Xianmin Elefant, Felice |
author_facet | Lorbeck, Meridith Pirooznia, Keerthy Sarthi, Jessica Zhu, Xianmin Elefant, Felice |
author_sort | Lorbeck, Meridith |
collection | PubMed |
description | BACKGROUND: Tip60 is a key histone acetyltransferase (HAT) enzyme that plays a central role in diverse biological processes critical for general cell function; however, the chromatin-mediated cell-type specific developmental pathways that are dependent exclusively upon the HAT activity of Tip60 remain to be explored. METHODS AND FINDINGS: Here, we investigate the role of Tip60 HAT activity in transcriptional control during multicellular development in vivo by examining genome-wide changes in gene expression in a Drosophila model system specifically depleted for endogenous dTip60 HAT function. CONCLUSIONS: We show that amino acid residue E431 in the catalytic HAT domain of dTip60 is critical for the acetylation of endogenous histone H4 in our fly model in vivo, and demonstrate that dTip60 HAT activity is essential for multicellular development. Moreover, our results uncover a novel role for Tip60 HAT activity in controlling neuronal specific gene expression profiles essential for nervous system function as well as a central regulatory role for Tip60 HAT function in general metabolism. |
format | Text |
id | pubmed-3073973 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-30739732011-04-14 Microarray Analysis Uncovers a Role for Tip60 in Nervous System Function and General Metabolism Lorbeck, Meridith Pirooznia, Keerthy Sarthi, Jessica Zhu, Xianmin Elefant, Felice PLoS One Research Article BACKGROUND: Tip60 is a key histone acetyltransferase (HAT) enzyme that plays a central role in diverse biological processes critical for general cell function; however, the chromatin-mediated cell-type specific developmental pathways that are dependent exclusively upon the HAT activity of Tip60 remain to be explored. METHODS AND FINDINGS: Here, we investigate the role of Tip60 HAT activity in transcriptional control during multicellular development in vivo by examining genome-wide changes in gene expression in a Drosophila model system specifically depleted for endogenous dTip60 HAT function. CONCLUSIONS: We show that amino acid residue E431 in the catalytic HAT domain of dTip60 is critical for the acetylation of endogenous histone H4 in our fly model in vivo, and demonstrate that dTip60 HAT activity is essential for multicellular development. Moreover, our results uncover a novel role for Tip60 HAT activity in controlling neuronal specific gene expression profiles essential for nervous system function as well as a central regulatory role for Tip60 HAT function in general metabolism. Public Library of Science 2011-04-11 /pmc/articles/PMC3073973/ /pubmed/21494552 http://dx.doi.org/10.1371/journal.pone.0018412 Text en Lorbeck et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Lorbeck, Meridith Pirooznia, Keerthy Sarthi, Jessica Zhu, Xianmin Elefant, Felice Microarray Analysis Uncovers a Role for Tip60 in Nervous System Function and General Metabolism |
title | Microarray Analysis Uncovers a Role for Tip60 in Nervous System Function and General Metabolism |
title_full | Microarray Analysis Uncovers a Role for Tip60 in Nervous System Function and General Metabolism |
title_fullStr | Microarray Analysis Uncovers a Role for Tip60 in Nervous System Function and General Metabolism |
title_full_unstemmed | Microarray Analysis Uncovers a Role for Tip60 in Nervous System Function and General Metabolism |
title_short | Microarray Analysis Uncovers a Role for Tip60 in Nervous System Function and General Metabolism |
title_sort | microarray analysis uncovers a role for tip60 in nervous system function and general metabolism |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3073973/ https://www.ncbi.nlm.nih.gov/pubmed/21494552 http://dx.doi.org/10.1371/journal.pone.0018412 |
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