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Human Dental Pulp Stem Cells Hook into Biocoral Scaffold Forming an Engineered Biocomplex

The aim of this study was to evaluate the behavior of human Dental Pulp Stem Cells (DPSCs), as well as human osteoblasts, when challenged on a Biocoral scaffold, which is a porous natural hydroxyapatite. For this purpose, human DPSCs were seeded onto a three-dimensional (3D) Biocoral scaffold or on...

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Autores principales: Mangano, Carlo, Paino, Francesca, d'Aquino, Riccardo, De Rosa, Alfredo, Iezzi, Giovanna, Piattelli, Adriano, Laino, Luigi, Mitsiadis, Thimios, Desiderio, Vincenzo, Mangano, Francesco, Papaccio, Gianpaolo, Tirino, Virginia
Formato: Texto
Lenguaje:English
Publicado: Public Library of Science 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3073992/
https://www.ncbi.nlm.nih.gov/pubmed/21494568
http://dx.doi.org/10.1371/journal.pone.0018721
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author Mangano, Carlo
Paino, Francesca
d'Aquino, Riccardo
De Rosa, Alfredo
Iezzi, Giovanna
Piattelli, Adriano
Laino, Luigi
Mitsiadis, Thimios
Desiderio, Vincenzo
Mangano, Francesco
Papaccio, Gianpaolo
Tirino, Virginia
author_facet Mangano, Carlo
Paino, Francesca
d'Aquino, Riccardo
De Rosa, Alfredo
Iezzi, Giovanna
Piattelli, Adriano
Laino, Luigi
Mitsiadis, Thimios
Desiderio, Vincenzo
Mangano, Francesco
Papaccio, Gianpaolo
Tirino, Virginia
author_sort Mangano, Carlo
collection PubMed
description The aim of this study was to evaluate the behavior of human Dental Pulp Stem Cells (DPSCs), as well as human osteoblasts, when challenged on a Biocoral scaffold, which is a porous natural hydroxyapatite. For this purpose, human DPSCs were seeded onto a three-dimensional (3D) Biocoral scaffold or on flask surface (control). Either normal or rotative (3D) cultures were performed. Scanning electron microscopic analyses, at 8, 24 and 48 h of culture showed that cells did not adhere on the external surface, but moved into the cavities inside the Biocoral structure. After 7, 15 and 30 days of culture, morphological and molecular analyses suggested that the Biocoral scaffold leads DPSCs to hook into the cavities where these cells quickly start to secrete the extra cellular matrix (ECM) and differentiate into osteoblasts. Control human osteoblasts also moved into the internal cavities where they secreted the ECM. Histological sections revealed a diffuse bone formation inside the Biocoral samples seeded with DPSCs or human osteoblasts, where the original scaffold and the new secreted biomaterial were completely integrated and cells were found within the remaining cavities. In addition, RT-PCR analyses showed a significant increase of osteoblast-related gene expression and, above all, of those genes highly expressed in mineralized tissues, including osteocalcin, OPN and BSP. Furthermore, the effects on the interaction between osteogenesis and angiogenesis were observed and substantiated by ELISA assays. Taken together, our results provide clear evidence that DPSCs differentiated into osteoblasts, forming a biocomplex made of Biocoral, ECM and differentiated cells.
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spelling pubmed-30739922011-04-14 Human Dental Pulp Stem Cells Hook into Biocoral Scaffold Forming an Engineered Biocomplex Mangano, Carlo Paino, Francesca d'Aquino, Riccardo De Rosa, Alfredo Iezzi, Giovanna Piattelli, Adriano Laino, Luigi Mitsiadis, Thimios Desiderio, Vincenzo Mangano, Francesco Papaccio, Gianpaolo Tirino, Virginia PLoS One Research Article The aim of this study was to evaluate the behavior of human Dental Pulp Stem Cells (DPSCs), as well as human osteoblasts, when challenged on a Biocoral scaffold, which is a porous natural hydroxyapatite. For this purpose, human DPSCs were seeded onto a three-dimensional (3D) Biocoral scaffold or on flask surface (control). Either normal or rotative (3D) cultures were performed. Scanning electron microscopic analyses, at 8, 24 and 48 h of culture showed that cells did not adhere on the external surface, but moved into the cavities inside the Biocoral structure. After 7, 15 and 30 days of culture, morphological and molecular analyses suggested that the Biocoral scaffold leads DPSCs to hook into the cavities where these cells quickly start to secrete the extra cellular matrix (ECM) and differentiate into osteoblasts. Control human osteoblasts also moved into the internal cavities where they secreted the ECM. Histological sections revealed a diffuse bone formation inside the Biocoral samples seeded with DPSCs or human osteoblasts, where the original scaffold and the new secreted biomaterial were completely integrated and cells were found within the remaining cavities. In addition, RT-PCR analyses showed a significant increase of osteoblast-related gene expression and, above all, of those genes highly expressed in mineralized tissues, including osteocalcin, OPN and BSP. Furthermore, the effects on the interaction between osteogenesis and angiogenesis were observed and substantiated by ELISA assays. Taken together, our results provide clear evidence that DPSCs differentiated into osteoblasts, forming a biocomplex made of Biocoral, ECM and differentiated cells. Public Library of Science 2011-04-11 /pmc/articles/PMC3073992/ /pubmed/21494568 http://dx.doi.org/10.1371/journal.pone.0018721 Text en Mangano et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Mangano, Carlo
Paino, Francesca
d'Aquino, Riccardo
De Rosa, Alfredo
Iezzi, Giovanna
Piattelli, Adriano
Laino, Luigi
Mitsiadis, Thimios
Desiderio, Vincenzo
Mangano, Francesco
Papaccio, Gianpaolo
Tirino, Virginia
Human Dental Pulp Stem Cells Hook into Biocoral Scaffold Forming an Engineered Biocomplex
title Human Dental Pulp Stem Cells Hook into Biocoral Scaffold Forming an Engineered Biocomplex
title_full Human Dental Pulp Stem Cells Hook into Biocoral Scaffold Forming an Engineered Biocomplex
title_fullStr Human Dental Pulp Stem Cells Hook into Biocoral Scaffold Forming an Engineered Biocomplex
title_full_unstemmed Human Dental Pulp Stem Cells Hook into Biocoral Scaffold Forming an Engineered Biocomplex
title_short Human Dental Pulp Stem Cells Hook into Biocoral Scaffold Forming an Engineered Biocomplex
title_sort human dental pulp stem cells hook into biocoral scaffold forming an engineered biocomplex
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3073992/
https://www.ncbi.nlm.nih.gov/pubmed/21494568
http://dx.doi.org/10.1371/journal.pone.0018721
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