Reconstitution of human RNA interference in budding yeast

Although RNA-mediated interference (RNAi) is a widely conserved process among eukaryotes, including many fungi, it is absent from the budding yeast Saccharomyces cerevisiae. Three human proteins, Ago2, Dicer and TRBP, are sufficient for reconstituting the RISC complex in vitro. To examine whether th...

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Detalles Bibliográficos
Autores principales: Suk, Kyoungho, Choi, Jihye, Suzuki, Yo, Ozturk, Sedide B., Mellor, Joseph C., Wong, Koon Ho, MacKay, Joanna L., Gregory, Richard I., Roth, Frederick P.
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2011
Materias:
Methods Online
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3074155/
https://www.ncbi.nlm.nih.gov/pubmed/21252293
http://dx.doi.org/10.1093/nar/gkq1321

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3074155/
https://www.ncbi.nlm.nih.gov/pubmed/21252293
http://dx.doi.org/10.1093/nar/gkq1321

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