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Synthesis of fluorapatite–hydroxyapatite nanoparticles and toxicity investigations
In this study, calcium phosphate nanoparticles with two phases, fluorapatite (FA; Ca(10)(PO(4))(6)F(2)) and hydroxyapatite (HA; Ca(10)(PO(4))(6)(OH)(2)), were prepared using the solgel method. Ethyl phosphate, hydrated calcium nitrate, and ammonium fluoride were used, respectively, as P, Ca, and F p...
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Formato: | Texto |
Lenguaje: | English |
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Dove Medical Press
2011
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3075893/ https://www.ncbi.nlm.nih.gov/pubmed/21499417 http://dx.doi.org/10.2147/IJN.S15461 |
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author | Montazeri, N Jahandideh, R Biazar, Esmaeil |
author_facet | Montazeri, N Jahandideh, R Biazar, Esmaeil |
author_sort | Montazeri, N |
collection | PubMed |
description | In this study, calcium phosphate nanoparticles with two phases, fluorapatite (FA; Ca(10)(PO(4))(6)F(2)) and hydroxyapatite (HA; Ca(10)(PO(4))(6)(OH)(2)), were prepared using the solgel method. Ethyl phosphate, hydrated calcium nitrate, and ammonium fluoride were used, respectively, as P, Ca, and F precursors with a Ca:P ratio of 1:72. Powders obtained from the sol-gel process were studied after they were dried at 80°C and heat treated at 550°C. The degree of crystallinity, particle and crystallite size, powder morphology, chemical structure, and phase analysis were investigated by scanning electron microscopy (SEM), X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), and Zetasizer experiments. The results of XRD analysis and FTIR showed the presence of hydroxyapatite and fluorapatite phases. The sizes of the crystallites estimated from XRD patterns using the Scherrer equation and the crystallinity of the hydroxyapatite phase were about 20 nm and 70%, respectively. Transmission electron microscope and SEM images and Zetasizer experiments showed an average size of 100 nm. The in vitro behavior of powder was investigated with mouse fibroblast cells. The results of these experiments indicated that the powders were biocompatibile and would not cause toxic reactions. These compounds could be applied for hard-tissue engineering. |
format | Text |
id | pubmed-3075893 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | Dove Medical Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-30758932011-04-15 Synthesis of fluorapatite–hydroxyapatite nanoparticles and toxicity investigations Montazeri, N Jahandideh, R Biazar, Esmaeil Int J Nanomedicine Original Research In this study, calcium phosphate nanoparticles with two phases, fluorapatite (FA; Ca(10)(PO(4))(6)F(2)) and hydroxyapatite (HA; Ca(10)(PO(4))(6)(OH)(2)), were prepared using the solgel method. Ethyl phosphate, hydrated calcium nitrate, and ammonium fluoride were used, respectively, as P, Ca, and F precursors with a Ca:P ratio of 1:72. Powders obtained from the sol-gel process were studied after they were dried at 80°C and heat treated at 550°C. The degree of crystallinity, particle and crystallite size, powder morphology, chemical structure, and phase analysis were investigated by scanning electron microscopy (SEM), X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), and Zetasizer experiments. The results of XRD analysis and FTIR showed the presence of hydroxyapatite and fluorapatite phases. The sizes of the crystallites estimated from XRD patterns using the Scherrer equation and the crystallinity of the hydroxyapatite phase were about 20 nm and 70%, respectively. Transmission electron microscope and SEM images and Zetasizer experiments showed an average size of 100 nm. The in vitro behavior of powder was investigated with mouse fibroblast cells. The results of these experiments indicated that the powders were biocompatibile and would not cause toxic reactions. These compounds could be applied for hard-tissue engineering. Dove Medical Press 2011 2011-01-28 /pmc/articles/PMC3075893/ /pubmed/21499417 http://dx.doi.org/10.2147/IJN.S15461 Text en © 2011 Montazeri, et al publisher and licensee Dove Medical Press Ltd. This is an Open Access article which permits unrestricted noncommercial use, provided the original work is properly cited. |
spellingShingle | Original Research Montazeri, N Jahandideh, R Biazar, Esmaeil Synthesis of fluorapatite–hydroxyapatite nanoparticles and toxicity investigations |
title | Synthesis of fluorapatite–hydroxyapatite nanoparticles and toxicity investigations |
title_full | Synthesis of fluorapatite–hydroxyapatite nanoparticles and toxicity investigations |
title_fullStr | Synthesis of fluorapatite–hydroxyapatite nanoparticles and toxicity investigations |
title_full_unstemmed | Synthesis of fluorapatite–hydroxyapatite nanoparticles and toxicity investigations |
title_short | Synthesis of fluorapatite–hydroxyapatite nanoparticles and toxicity investigations |
title_sort | synthesis of fluorapatite–hydroxyapatite nanoparticles and toxicity investigations |
topic | Original Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3075893/ https://www.ncbi.nlm.nih.gov/pubmed/21499417 http://dx.doi.org/10.2147/IJN.S15461 |
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