Cargando…
Preparation, characterization and targeting of micronized 10-hydroxycamptothecin-loaded folate-conjugated human serum albumin nanoparticles to cancer cells
BACKGROUND: The purpose of this study was to develop a method for targeted delivery of 10-hydroxycamptothecin (HCPT)-loaded nanoparticles (NPs) to cancer cells. METHODS: We first used a supercritical antisolvent process to prepare micronized HCPT (nHCPT), and then folate-conjugated human serum album...
Autores principales: | , , , , , , , , , , |
---|---|
Formato: | Texto |
Lenguaje: | English |
Publicado: |
Dove Medical Press
2011
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3075905/ https://www.ncbi.nlm.nih.gov/pubmed/21499429 http://dx.doi.org/10.2147/IJN.S16144 |
_version_ | 1782201796730552320 |
---|---|
author | Li, Qingyong Liu, Chen Zhao, Xiuhua Zu, Yuangang Wang, Ying Zhang, Baoyou Zhao, Dongmei Zhao, Qi Su, Lin Gao, Yang Sun, Baihe |
author_facet | Li, Qingyong Liu, Chen Zhao, Xiuhua Zu, Yuangang Wang, Ying Zhang, Baoyou Zhao, Dongmei Zhao, Qi Su, Lin Gao, Yang Sun, Baihe |
author_sort | Li, Qingyong |
collection | PubMed |
description | BACKGROUND: The purpose of this study was to develop a method for targeted delivery of 10-hydroxycamptothecin (HCPT)-loaded nanoparticles (NPs) to cancer cells. METHODS: We first used a supercritical antisolvent process to prepare micronized HCPT (nHCPT), and then folate-conjugated human serum albumin (HSA) nHCPT-loaded NPs (FA-HSA-nHCPT-NPs) were prepared using a NP-coated method combined with a desolvation technique. The amount of folate conjugation was 16 μg · mg(−1) HSA. RESULTS: The particle size of the spherical nHCPT microparticles obtained was 118.5 ± 6.6 nm. The particle size and zeta potential of the FA-HSA-nHCPT-NPs were 233.9 ± 1.2 nm and −25.23 ± 2.98 mV, respectively. The FA-HSA-nHCPT-NPs exhibited a smooth surface and a distinct spherical shape, and the results of differential scanning calorimetry and X-ray diffraction indicated that the FA-HSA-nHCPT-NPs presented in a nanostructured amorphous state. The FA-HSA-nHCPT-NPs showed sustained-release characteristics for 120 hours in vitro, with a drug-loading content of 7.3% and an encapsulating efficiency of 79.1%. CONCLUSION: The FA-NPs were effective delivery systems for uptake by SGC7901 cells compared with folate-free NPs. These results suggest that a NP-coated method combined with a desolvation technique is effective for preparing NPs with drugs having poor solubility in water and most organic solvents, using albumin as the wall material. FA-HSA-NPs are a stable delivery system and have the potential for targeted delivery of anticancer drugs. |
format | Text |
id | pubmed-3075905 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | Dove Medical Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-30759052011-04-15 Preparation, characterization and targeting of micronized 10-hydroxycamptothecin-loaded folate-conjugated human serum albumin nanoparticles to cancer cells Li, Qingyong Liu, Chen Zhao, Xiuhua Zu, Yuangang Wang, Ying Zhang, Baoyou Zhao, Dongmei Zhao, Qi Su, Lin Gao, Yang Sun, Baihe Int J Nanomedicine Original Research BACKGROUND: The purpose of this study was to develop a method for targeted delivery of 10-hydroxycamptothecin (HCPT)-loaded nanoparticles (NPs) to cancer cells. METHODS: We first used a supercritical antisolvent process to prepare micronized HCPT (nHCPT), and then folate-conjugated human serum albumin (HSA) nHCPT-loaded NPs (FA-HSA-nHCPT-NPs) were prepared using a NP-coated method combined with a desolvation technique. The amount of folate conjugation was 16 μg · mg(−1) HSA. RESULTS: The particle size of the spherical nHCPT microparticles obtained was 118.5 ± 6.6 nm. The particle size and zeta potential of the FA-HSA-nHCPT-NPs were 233.9 ± 1.2 nm and −25.23 ± 2.98 mV, respectively. The FA-HSA-nHCPT-NPs exhibited a smooth surface and a distinct spherical shape, and the results of differential scanning calorimetry and X-ray diffraction indicated that the FA-HSA-nHCPT-NPs presented in a nanostructured amorphous state. The FA-HSA-nHCPT-NPs showed sustained-release characteristics for 120 hours in vitro, with a drug-loading content of 7.3% and an encapsulating efficiency of 79.1%. CONCLUSION: The FA-NPs were effective delivery systems for uptake by SGC7901 cells compared with folate-free NPs. These results suggest that a NP-coated method combined with a desolvation technique is effective for preparing NPs with drugs having poor solubility in water and most organic solvents, using albumin as the wall material. FA-HSA-NPs are a stable delivery system and have the potential for targeted delivery of anticancer drugs. Dove Medical Press 2011 2011-02-20 /pmc/articles/PMC3075905/ /pubmed/21499429 http://dx.doi.org/10.2147/IJN.S16144 Text en © 2011 Li et al, publisher and licensee Dove Medical Press Ltd. This is an Open Access article which permits unrestricted noncommercial use, provided the original work is properly cited. |
spellingShingle | Original Research Li, Qingyong Liu, Chen Zhao, Xiuhua Zu, Yuangang Wang, Ying Zhang, Baoyou Zhao, Dongmei Zhao, Qi Su, Lin Gao, Yang Sun, Baihe Preparation, characterization and targeting of micronized 10-hydroxycamptothecin-loaded folate-conjugated human serum albumin nanoparticles to cancer cells |
title | Preparation, characterization and targeting of micronized 10-hydroxycamptothecin-loaded folate-conjugated human serum albumin nanoparticles to cancer cells |
title_full | Preparation, characterization and targeting of micronized 10-hydroxycamptothecin-loaded folate-conjugated human serum albumin nanoparticles to cancer cells |
title_fullStr | Preparation, characterization and targeting of micronized 10-hydroxycamptothecin-loaded folate-conjugated human serum albumin nanoparticles to cancer cells |
title_full_unstemmed | Preparation, characterization and targeting of micronized 10-hydroxycamptothecin-loaded folate-conjugated human serum albumin nanoparticles to cancer cells |
title_short | Preparation, characterization and targeting of micronized 10-hydroxycamptothecin-loaded folate-conjugated human serum albumin nanoparticles to cancer cells |
title_sort | preparation, characterization and targeting of micronized 10-hydroxycamptothecin-loaded folate-conjugated human serum albumin nanoparticles to cancer cells |
topic | Original Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3075905/ https://www.ncbi.nlm.nih.gov/pubmed/21499429 http://dx.doi.org/10.2147/IJN.S16144 |
work_keys_str_mv | AT liqingyong preparationcharacterizationandtargetingofmicronized10hydroxycamptothecinloadedfolateconjugatedhumanserumalbuminnanoparticlestocancercells AT liuchen preparationcharacterizationandtargetingofmicronized10hydroxycamptothecinloadedfolateconjugatedhumanserumalbuminnanoparticlestocancercells AT zhaoxiuhua preparationcharacterizationandtargetingofmicronized10hydroxycamptothecinloadedfolateconjugatedhumanserumalbuminnanoparticlestocancercells AT zuyuangang preparationcharacterizationandtargetingofmicronized10hydroxycamptothecinloadedfolateconjugatedhumanserumalbuminnanoparticlestocancercells AT wangying preparationcharacterizationandtargetingofmicronized10hydroxycamptothecinloadedfolateconjugatedhumanserumalbuminnanoparticlestocancercells AT zhangbaoyou preparationcharacterizationandtargetingofmicronized10hydroxycamptothecinloadedfolateconjugatedhumanserumalbuminnanoparticlestocancercells AT zhaodongmei preparationcharacterizationandtargetingofmicronized10hydroxycamptothecinloadedfolateconjugatedhumanserumalbuminnanoparticlestocancercells AT zhaoqi preparationcharacterizationandtargetingofmicronized10hydroxycamptothecinloadedfolateconjugatedhumanserumalbuminnanoparticlestocancercells AT sulin preparationcharacterizationandtargetingofmicronized10hydroxycamptothecinloadedfolateconjugatedhumanserumalbuminnanoparticlestocancercells AT gaoyang preparationcharacterizationandtargetingofmicronized10hydroxycamptothecinloadedfolateconjugatedhumanserumalbuminnanoparticlestocancercells AT sunbaihe preparationcharacterizationandtargetingofmicronized10hydroxycamptothecinloadedfolateconjugatedhumanserumalbuminnanoparticlestocancercells |