Cargando…

Structural studies of the mechanism for biosensing antibiotics in a fluorescein-labeled β-lactamase

BACKGROUND: β-lactamase conjugated with environment-sensitive fluorescein molecule to residue 166 on the Ω-loop near its catalytic site is a highly effective biosensor for β-lactam antibiotics. Yet the molecular mechanism of such fluorescence-based biosensing is not well understood. RESULTS: Here we...

Descripción completa

Detalles Bibliográficos
Autores principales:	Wong, Wai-Ting, Au, Ho-Wah, Yap, Hong-Kin, Leung, Yun-Chung, Wong, Kwok-Yin, Zhao, Yanxiang
Formato:	Texto
Lenguaje:	English
Publicado:	BioMed Central 2011
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3076226/ https://www.ncbi.nlm.nih.gov/pubmed/21443768 http://dx.doi.org/10.1186/1472-6807-11-15

Ejemplares similares

Thermostable β-Lactamase Mutant with Its Active Site Conjugated with Fluorescein for Efficient β-Lactam Antibiotic Detection
por: Au, Ho-Wah, et al.
Publicado: (2019)

BADAN-conjugated β-lactamases as biosensors for β-lactam antibiotic detection
por: Au, Ho-Wah, et al.
Publicado: (2020)

Method Based on the β-Lactamase PenPC Fluorescent Labeled for β-Lactam Antibiotic Quantification in Human Plasma
por: Andresen, Max, et al.
Publicado: (2016)

Efficient production of secretory Streptomyces clavuligerus β-lactamase inhibitory protein (BLIP) in Pichia pastoris
por: Law, Kin-Ho, et al.
Publicado: (2018)

Detection of SHV β-lactamases in Gram-negative bacilli using fluorescein-labeled antibodies
por: Hujer, Andrea M, et al.
Publicado: (2009)

Effect of Lactamase Inhibitors on the Biosensor Penp during the Measurement of Lactam Antibiotics Concentration
por: Soto, Dagoberto, et al.
Publicado: (2019)

Interdomain flexibility and interfacial integrity of β-lactamase inhibitory protein (BLIP) modulate its binding to class A β-lactamases
por: Huang, Liwen, et al.
Publicado: (2021)

Evaluation of Meropenem Pharmacokinetics in an Experimental Acute Respiratory Distress Syndrome (ARDS) Model during Extracorporeal Membrane Oxygenation (ECMO) by Using a PenP β-Lactamase Biosensor
por: Andresen, Max, et al.
Publicado: (2018)

Fluorescent TEM-1 β-lactamase with wild-type activity as a rapid drug sensor for in vitro drug screening
por: Cheong, Wing-Lam, et al.
Publicado: (2014)

Specific Amino Acid Substitutions in OXA-51-Type β-Lactamase Enhance Catalytic Activity to a Level Comparable to Carbapenemase OXA-23 and OXA-24/40
por: Chan, Kwan-Wai, et al.
Publicado: (2022)

Sodium Fluorescein as an Optical Label to Evaluate Subretinal Injection
por: Simunovic, Matthew P., et al.
Publicado: (2020)

Honey-Induced Protein Stabilization as Studied by Fluorescein Isothiocyanate Fluorescence
por: Wong, Yin How, et al.
Publicado: (2013)

Surface-Bound Antibiotic for the Detection of β-Lactamases
por: Miller, Lisa M., et al.
Publicado: (2019)

Facile synthesis of N-rich carbon quantum dots from porphyrins as efficient probes for bioimaging and biosensing in living cells
por: Wu, Fengshou, et al.
Publicado: (2017)

Antibiotic Susceptibility Testing with Raman Biosensing
por: Novikov, Andrei, et al.
Publicado: (2022)

THE USE OF FLUORESCEIN-LABELED HEAVY MEROMYOSIN FOR THE CYTOLOGICAL DEMONSTRATION OF ACTIN
por: Aronson, John F.
Publicado: (1965)

Direct visualization of fluorescein-labeled microtubules in vitro and in microinjected fibroblasts
Publicado: (1981)

Rapid optical determination of β-lactamase and antibiotic activity
por: Khan, Shazia, et al.
Publicado: (2014)

Tackling the Antibiotic Resistance Caused by Class A β-Lactamases through the Use of β-Lactamase Inhibitory Protein
por: Eiamphungporn, Warawan, et al.
Publicado: (2018)

Homogeneous Biosensing Based on Magnetic Particle Labels
por: Schrittwieser, Stefan, et al.
Publicado: (2016)

The structural dynamics of full-length divisome transmembrane proteins FtsQ, FtsB, and FtsL in FtsQBL complex formation
por: Kong, Wai-Po, et al.
Publicado: (2022)

Multiplex Digital Quantification of β-Lactamase Genes in Antibiotic-Resistant Bacteria by Counting Gold Nanoparticle Labels on Silicon Microchips
por: Presnova, Galina V., et al.
Publicado: (2022)

Effect on muscle strength after blood flow restriction resistance exercise in early in-patient rehabilitation of post-chronic obstructive pulmonary disease acute exacerbation, a single blinded, randomized controlled study
por: Lau, Chung Wai, et al.
Publicado: (2023)

THE DISTRIBUTION OF MUSCLE ANTIGENS IN CONTRACTED MYOFIBRILS DETERMINED BY FLUORESCEIN-LABELED ANTIBODIES
por: Tunik, Bernard, et al.
Publicado: (1961)

Antibiotic-functionalized gold nanoparticles for the detection of active β-lactamases
por: Miller, Lisa M., et al.
Publicado: (2021)

Bactericidal Antibiotics Increase Hydroxyphenyl Fluorescein Signal by Altering Cell Morphology
por: Paulander, Wilhelm, et al.
Publicado: (2014)

A self-assembled plasmonic optical fiber nanoprobe for label-free biosensing
por: Liang, Yuzhang, et al.
Publicado: (2019)

Structural Variabilities in β-Lactamase (blaA) of Different Biovars of Yersinia enterocolitica: Implications for β-Lactam Antibiotic and β-Lactamase Inhibitor Susceptibilities
por: Singhal, Neelja, et al.
Publicado: (2015)

Fluorescein cap: Fluorescein angiographic feature of retinal cavernous hemangioma
por: Kumar, Madhu, et al.
Publicado: (2018)

Prenatal sonography of placental abscess and prolonged antibiotic treatment for Serratia marcescens bacteremia
por: Mak, Annisa Shui Lam, et al.
Publicado: (2018)

Assembly properties of fluorescein-labeled tubulin in vitro before and after fluorescence bleaching
Publicado: (1984)

269. De-Labeling of Allergies to Β-Lactam Antibiotics (De-LABeL) Program: Development and Pilot of an Inpatient Pediatric Program
por: Wong, Jacqueline, et al.
Publicado: (2018)

The Importance of Porins and β-Lactamase in Outer Membrane Vesicles on the Hydrolysis of β-Lactam Antibiotics
por: Kim, Si Won, et al.
Publicado: (2020)

Extended spectrum β-lactamase producing Enterobacteriaceae & antibiotic co-resistance
por: Rudresh, S.M., et al.
Publicado: (2011)

AMA overcomes antibiotic resistance by NDM and VIM metallo-β-lactamases
por: King, Andrew M., et al.
Publicado: (2014)

Broad spectrum antibiotic-degrading metallo-β-lactamases are phylogenetically diverse
por: Pedroso, Marcelo Monteiro, et al.
Publicado: (2020)

A metallo-β-lactamase enzyme for internal detoxification of the antibiotic thienamycin
por: Diene, Seydina M., et al.
Publicado: (2021)

Who are less likely to return to work after getting injured on duty? A 12‐month epidemiological evaluation in an orthopedic and traumatology center in Hong Kong
por: Wong, Daniel Wai‐yip, et al.
Publicado: (2021)

Mono-PEGylation of a Thermostable Arginine-Depleting Enzyme for the Treatment of Lung Cancer
por: Chung, Sai-Fung, et al.
Publicado: (2020)

Rapid Detection of Multiple Classes of β-Lactam Antibiotics in Blood Using an NDM-1 Biosensing Assay
por: Meng, Qinglai, et al.
Publicado: (2021)

Cannot write session to /tmp/vufind_sessions/sess_o8mc9pscdp085ctbtfqduajr4m