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Porphyromonas gingivalis Participates in Pathogenesis of Human Abdominal Aortic Aneurysm by Neutrophil Activation. Proof of Concept in Rats

BACKGROUND: Abdominal Aortic Aneurysms (AAAs) represent a particular form of atherothrombosis where neutrophil proteolytic activity plays a major role. We postulated that neutrophil recruitment and activation participating in AAA growth may originate in part from repeated episodes of periodontal bac...

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Autores principales: Delbosc, Sandrine, Alsac, Jean-Marc, Journe, Clement, Louedec, Liliane, Castier, Yves, Bonnaure-Mallet, Martine, Ruimy, Raymond, Rossignol, Patrick, Bouchard, Philippe, Michel, Jean-Baptiste, Meilhac, Olivier
Formato: Texto
Lenguaje:English
Publicado: Public Library of Science 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3076426/
https://www.ncbi.nlm.nih.gov/pubmed/21533243
http://dx.doi.org/10.1371/journal.pone.0018679
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author Delbosc, Sandrine
Alsac, Jean-Marc
Journe, Clement
Louedec, Liliane
Castier, Yves
Bonnaure-Mallet, Martine
Ruimy, Raymond
Rossignol, Patrick
Bouchard, Philippe
Michel, Jean-Baptiste
Meilhac, Olivier
author_facet Delbosc, Sandrine
Alsac, Jean-Marc
Journe, Clement
Louedec, Liliane
Castier, Yves
Bonnaure-Mallet, Martine
Ruimy, Raymond
Rossignol, Patrick
Bouchard, Philippe
Michel, Jean-Baptiste
Meilhac, Olivier
author_sort Delbosc, Sandrine
collection PubMed
description BACKGROUND: Abdominal Aortic Aneurysms (AAAs) represent a particular form of atherothrombosis where neutrophil proteolytic activity plays a major role. We postulated that neutrophil recruitment and activation participating in AAA growth may originate in part from repeated episodes of periodontal bacteremia. METHODS AND FINDINGS: Our results show that neutrophil activation in human AAA was associated with Neutrophil Extracellular Trap (NET) formation in the IntraLuminal Thrombus, leading to the release of cell-free DNA. Human AAA samples were shown to contain bacterial DNA with high frequency (11/16), and in particular that of Porphyromonas gingivalis (Pg), the most prevalent pathogen involved in chronic periodontitis, a common form of periodontal disease. Both DNA reflecting the presence of NETs and antibodies to Pg were found to be increased in plasma of patients with AAA. Using a rat model of AAA, we demonstrated that repeated injection of Pg fostered aneurysm development, associated with pathological characteristics similar to those observed in humans, such as the persistence of a neutrophil-rich luminal thrombus, not observed in saline-injected rats in which a healing process was observed. CONCLUSIONS: Thus, the control of periodontal disease may represent a therapeutic target to limit human AAA progression.
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spelling pubmed-30764262011-04-29 Porphyromonas gingivalis Participates in Pathogenesis of Human Abdominal Aortic Aneurysm by Neutrophil Activation. Proof of Concept in Rats Delbosc, Sandrine Alsac, Jean-Marc Journe, Clement Louedec, Liliane Castier, Yves Bonnaure-Mallet, Martine Ruimy, Raymond Rossignol, Patrick Bouchard, Philippe Michel, Jean-Baptiste Meilhac, Olivier PLoS One Research Article BACKGROUND: Abdominal Aortic Aneurysms (AAAs) represent a particular form of atherothrombosis where neutrophil proteolytic activity plays a major role. We postulated that neutrophil recruitment and activation participating in AAA growth may originate in part from repeated episodes of periodontal bacteremia. METHODS AND FINDINGS: Our results show that neutrophil activation in human AAA was associated with Neutrophil Extracellular Trap (NET) formation in the IntraLuminal Thrombus, leading to the release of cell-free DNA. Human AAA samples were shown to contain bacterial DNA with high frequency (11/16), and in particular that of Porphyromonas gingivalis (Pg), the most prevalent pathogen involved in chronic periodontitis, a common form of periodontal disease. Both DNA reflecting the presence of NETs and antibodies to Pg were found to be increased in plasma of patients with AAA. Using a rat model of AAA, we demonstrated that repeated injection of Pg fostered aneurysm development, associated with pathological characteristics similar to those observed in humans, such as the persistence of a neutrophil-rich luminal thrombus, not observed in saline-injected rats in which a healing process was observed. CONCLUSIONS: Thus, the control of periodontal disease may represent a therapeutic target to limit human AAA progression. Public Library of Science 2011-04-13 /pmc/articles/PMC3076426/ /pubmed/21533243 http://dx.doi.org/10.1371/journal.pone.0018679 Text en Delbosc et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Delbosc, Sandrine
Alsac, Jean-Marc
Journe, Clement
Louedec, Liliane
Castier, Yves
Bonnaure-Mallet, Martine
Ruimy, Raymond
Rossignol, Patrick
Bouchard, Philippe
Michel, Jean-Baptiste
Meilhac, Olivier
Porphyromonas gingivalis Participates in Pathogenesis of Human Abdominal Aortic Aneurysm by Neutrophil Activation. Proof of Concept in Rats
title Porphyromonas gingivalis Participates in Pathogenesis of Human Abdominal Aortic Aneurysm by Neutrophil Activation. Proof of Concept in Rats
title_full Porphyromonas gingivalis Participates in Pathogenesis of Human Abdominal Aortic Aneurysm by Neutrophil Activation. Proof of Concept in Rats
title_fullStr Porphyromonas gingivalis Participates in Pathogenesis of Human Abdominal Aortic Aneurysm by Neutrophil Activation. Proof of Concept in Rats
title_full_unstemmed Porphyromonas gingivalis Participates in Pathogenesis of Human Abdominal Aortic Aneurysm by Neutrophil Activation. Proof of Concept in Rats
title_short Porphyromonas gingivalis Participates in Pathogenesis of Human Abdominal Aortic Aneurysm by Neutrophil Activation. Proof of Concept in Rats
title_sort porphyromonas gingivalis participates in pathogenesis of human abdominal aortic aneurysm by neutrophil activation. proof of concept in rats
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3076426/
https://www.ncbi.nlm.nih.gov/pubmed/21533243
http://dx.doi.org/10.1371/journal.pone.0018679
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