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A transgenic mouse for in vivo detection of endogenous labeled mRNA

Live-cell single mRNA imaging is a powerful tool, but has been restricted in higher eukaryotes to artificial cell lines and reporter genes. We describe an approach that enables live-cell imaging of single endogenous labeled mRNA molecules transcribed in primary mammalian cells and tissue. We generat...

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Detalles Bibliográficos
Autores principales:	Lionnet, Timothée, Czaplinski, Kevin, Darzacq, Xavier, Shav-Tal, Yaron, Wells, Amber L., Chao, Jeffrey A., Park, Hye Yoon, de Turris, Valeria, Lopez-Jones, Melissa, Singer, Robert H.
Formato:	Texto
Lenguaje:	English
Publicado:	2011
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3076588/ https://www.ncbi.nlm.nih.gov/pubmed/21240280 http://dx.doi.org/10.1038/nmeth.1551

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