Simultaneous 2-photon calcium imaging at different cortical depths in vivo with spatiotemporal multiplexing

In vivo 2-photon calcium imaging would benefit from the use of multiple excitation beams to increase scanning speed, signal-to-noise ratio, field of view, or to image different axial planes simultaneously. We adapted a spatiotemporal multiplexing approach to circumvent the problem of light scatterin...

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Autores principales: Cheng, Adrian, Gonçalves, J. Tiago, Golshani, Peyman, Arisaka, Katsushi, Portera-Cailliau, Carlos
Formato: Texto
Lenguaje:English
Publicado: 2011
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3076599/
https://www.ncbi.nlm.nih.gov/pubmed/21217749
http://dx.doi.org/10.1038/nmeth.1552
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author Cheng, Adrian
Gonçalves, J. Tiago
Golshani, Peyman
Arisaka, Katsushi
Portera-Cailliau, Carlos
author_facet Cheng, Adrian
Gonçalves, J. Tiago
Golshani, Peyman
Arisaka, Katsushi
Portera-Cailliau, Carlos
author_sort Cheng, Adrian
collection PubMed
description In vivo 2-photon calcium imaging would benefit from the use of multiple excitation beams to increase scanning speed, signal-to-noise ratio, field of view, or to image different axial planes simultaneously. We adapted a spatiotemporal multiplexing approach to circumvent the problem of light scattering ambiguity in deep tissue inherent to multifocal 2-photon microscopy. We demonstrate 2-photon calcium imaging at multiple axial planes in the in vivo mouse brain to monitor network activity of large ensembles of cortical neurons in three spatial dimensions.
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spelling pubmed-30765992011-08-01 Simultaneous 2-photon calcium imaging at different cortical depths in vivo with spatiotemporal multiplexing Cheng, Adrian Gonçalves, J. Tiago Golshani, Peyman Arisaka, Katsushi Portera-Cailliau, Carlos Nat Methods Article In vivo 2-photon calcium imaging would benefit from the use of multiple excitation beams to increase scanning speed, signal-to-noise ratio, field of view, or to image different axial planes simultaneously. We adapted a spatiotemporal multiplexing approach to circumvent the problem of light scattering ambiguity in deep tissue inherent to multifocal 2-photon microscopy. We demonstrate 2-photon calcium imaging at multiple axial planes in the in vivo mouse brain to monitor network activity of large ensembles of cortical neurons in three spatial dimensions. 2011-01-09 2011-02 /pmc/articles/PMC3076599/ /pubmed/21217749 http://dx.doi.org/10.1038/nmeth.1552 Text en Users may view, print, copy, download and text and data- mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use: http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Cheng, Adrian
Gonçalves, J. Tiago
Golshani, Peyman
Arisaka, Katsushi
Portera-Cailliau, Carlos
Simultaneous 2-photon calcium imaging at different cortical depths in vivo with spatiotemporal multiplexing
title Simultaneous 2-photon calcium imaging at different cortical depths in vivo with spatiotemporal multiplexing
title_full Simultaneous 2-photon calcium imaging at different cortical depths in vivo with spatiotemporal multiplexing
title_fullStr Simultaneous 2-photon calcium imaging at different cortical depths in vivo with spatiotemporal multiplexing
title_full_unstemmed Simultaneous 2-photon calcium imaging at different cortical depths in vivo with spatiotemporal multiplexing
title_short Simultaneous 2-photon calcium imaging at different cortical depths in vivo with spatiotemporal multiplexing
title_sort simultaneous 2-photon calcium imaging at different cortical depths in vivo with spatiotemporal multiplexing
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3076599/
https://www.ncbi.nlm.nih.gov/pubmed/21217749
http://dx.doi.org/10.1038/nmeth.1552
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