Allosteric Control of Ligand Binding Affinity Using Engineered Conformation-Specific Effector Proteins

We describe a phage display methodology to engineer synthetic antigen binders (sABs) that recognize either the apo- or the ligand-bound conformation of maltose binding protein (MBP). sABs that preferentially recognize the maltose-bound form of MBP act as positive allosteric effectors by significantl...

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Detalles Bibliográficos
Autores principales: Rizk, Shahir S., Paduch, Marcin, Heithaus, John H., Duguid, Erica M., Sandstrom, Andrew, Kossiakoff, Anthony A.
Formato: Texto
Lenguaje:English
Publicado: 2011
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3077571/
https://www.ncbi.nlm.nih.gov/pubmed/21378967
http://dx.doi.org/10.1038/nsmb.2002

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