Genome-wide identification of Ago2 binding sites from mouse embryonic stem cells with and without mature microRNAs

MicroRNAs (miRNAs) are 19-22nt non-coding RNAs that post-transcriptionally regulate mRNA targets. To identify endogenous miRNA binding sites, we performed photo-crosslinking immunoprecipitation using Ago2 antibodies, followed by deep-sequencing of RNAs (CLIP-seq) in mouse embryonic stem cells (mESCs...

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Detalles Bibliográficos
Autores principales: Leung, Anthony K L, Young, Amanda G, Bhutkar, Arjun, Zheng, Grace X, Bosson, Andrew D, Nielsen, Cydney B, Sharp, Phillip A
Formato: Texto
Lenguaje:English
Publicado: 2011
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3078052/
https://www.ncbi.nlm.nih.gov/pubmed/21258322
http://dx.doi.org/10.1038/nsmb.1991

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