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Arid5a cooperates with Sox9 to stimulate chondrocyte-specific transcription
SRY-box–containing gene 9 (Sox9) is an essential transcription factor in chondrocyte lineage determination and differentiation. Recent studies demonstrated that Sox9 controls the transcription of chondrocyte-specific genes in association with several other transcriptional regulators. To further unde...
Autores principales: | , , , , , , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
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The American Society for Cell Biology
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3078073/ https://www.ncbi.nlm.nih.gov/pubmed/21346191 http://dx.doi.org/10.1091/mbc.E10-07-0566 |
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author | Amano, Katsuhiko Hata, Kenji Muramatsu, Shuji Wakabayashi, Makoto Takigawa, Yoko Ono, Koichiro Nakanishi, Masako Takashima, Rikako Kogo, Mikihiko Matsuda, Akio Nishimura, Riko Yoneda, Toshiyuki |
author_facet | Amano, Katsuhiko Hata, Kenji Muramatsu, Shuji Wakabayashi, Makoto Takigawa, Yoko Ono, Koichiro Nakanishi, Masako Takashima, Rikako Kogo, Mikihiko Matsuda, Akio Nishimura, Riko Yoneda, Toshiyuki |
author_sort | Amano, Katsuhiko |
collection | PubMed |
description | SRY-box–containing gene 9 (Sox9) is an essential transcription factor in chondrocyte lineage determination and differentiation. Recent studies demonstrated that Sox9 controls the transcription of chondrocyte-specific genes in association with several other transcriptional regulators. To further understand the molecular mechanisms by which Sox9 influences transcriptional events during chondrocyte differentiation, we attempted to identify transcriptional partners of Sox9 and to examine their roles in chondrocyte differentiation. We isolated AT-rich interactive domain–containing protein 5a (Arid5a; also known as Mrf1) as an activator of the Col2a1 gene promoter from an ATDC5 cDNA library. Arid5a was highly expressed in cartilage and induced during chondrocyte differentiation. Furthermore, Arid5a physically interacted with Sox9 in nuclei and up-regulated the chondrocyte-specific action of Sox9. Overexpression of Arid5a stimulated chondrocyte differentiation in vitro and in an organ culture system. In contrast, Arid5a knockdown inhibited Col2a1 expression in chondrocytes. In addition, Arid5a binds directly to the promoter region of the Col2a1 gene and stimulates acetylation of histone 3 in the region. Our results suggest that Arid5a may directly interact with Sox9 and thereby enhance its chondrocyte-specific action. |
format | Text |
id | pubmed-3078073 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | The American Society for Cell Biology |
record_format | MEDLINE/PubMed |
spelling | pubmed-30780732011-06-30 Arid5a cooperates with Sox9 to stimulate chondrocyte-specific transcription Amano, Katsuhiko Hata, Kenji Muramatsu, Shuji Wakabayashi, Makoto Takigawa, Yoko Ono, Koichiro Nakanishi, Masako Takashima, Rikako Kogo, Mikihiko Matsuda, Akio Nishimura, Riko Yoneda, Toshiyuki Mol Biol Cell Articles SRY-box–containing gene 9 (Sox9) is an essential transcription factor in chondrocyte lineage determination and differentiation. Recent studies demonstrated that Sox9 controls the transcription of chondrocyte-specific genes in association with several other transcriptional regulators. To further understand the molecular mechanisms by which Sox9 influences transcriptional events during chondrocyte differentiation, we attempted to identify transcriptional partners of Sox9 and to examine their roles in chondrocyte differentiation. We isolated AT-rich interactive domain–containing protein 5a (Arid5a; also known as Mrf1) as an activator of the Col2a1 gene promoter from an ATDC5 cDNA library. Arid5a was highly expressed in cartilage and induced during chondrocyte differentiation. Furthermore, Arid5a physically interacted with Sox9 in nuclei and up-regulated the chondrocyte-specific action of Sox9. Overexpression of Arid5a stimulated chondrocyte differentiation in vitro and in an organ culture system. In contrast, Arid5a knockdown inhibited Col2a1 expression in chondrocytes. In addition, Arid5a binds directly to the promoter region of the Col2a1 gene and stimulates acetylation of histone 3 in the region. Our results suggest that Arid5a may directly interact with Sox9 and thereby enhance its chondrocyte-specific action. The American Society for Cell Biology 2011-04-15 /pmc/articles/PMC3078073/ /pubmed/21346191 http://dx.doi.org/10.1091/mbc.E10-07-0566 Text en © 2011 Amano et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0). “ASCB®,“ “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society of Cell Biology. |
spellingShingle | Articles Amano, Katsuhiko Hata, Kenji Muramatsu, Shuji Wakabayashi, Makoto Takigawa, Yoko Ono, Koichiro Nakanishi, Masako Takashima, Rikako Kogo, Mikihiko Matsuda, Akio Nishimura, Riko Yoneda, Toshiyuki Arid5a cooperates with Sox9 to stimulate chondrocyte-specific transcription |
title | Arid5a cooperates with Sox9 to stimulate chondrocyte-specific transcription |
title_full | Arid5a cooperates with Sox9 to stimulate chondrocyte-specific transcription |
title_fullStr | Arid5a cooperates with Sox9 to stimulate chondrocyte-specific transcription |
title_full_unstemmed | Arid5a cooperates with Sox9 to stimulate chondrocyte-specific transcription |
title_short | Arid5a cooperates with Sox9 to stimulate chondrocyte-specific transcription |
title_sort | arid5a cooperates with sox9 to stimulate chondrocyte-specific transcription |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3078073/ https://www.ncbi.nlm.nih.gov/pubmed/21346191 http://dx.doi.org/10.1091/mbc.E10-07-0566 |
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