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Arid5a cooperates with Sox9 to stimulate chondrocyte-specific transcription

SRY-box–containing gene 9 (Sox9) is an essential transcription factor in chondrocyte lineage determination and differentiation. Recent studies demonstrated that Sox9 controls the transcription of chondrocyte-specific genes in association with several other transcriptional regulators. To further unde...

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Autores principales: Amano, Katsuhiko, Hata, Kenji, Muramatsu, Shuji, Wakabayashi, Makoto, Takigawa, Yoko, Ono, Koichiro, Nakanishi, Masako, Takashima, Rikako, Kogo, Mikihiko, Matsuda, Akio, Nishimura, Riko, Yoneda, Toshiyuki
Formato: Texto
Lenguaje:English
Publicado: The American Society for Cell Biology 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3078073/
https://www.ncbi.nlm.nih.gov/pubmed/21346191
http://dx.doi.org/10.1091/mbc.E10-07-0566
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author Amano, Katsuhiko
Hata, Kenji
Muramatsu, Shuji
Wakabayashi, Makoto
Takigawa, Yoko
Ono, Koichiro
Nakanishi, Masako
Takashima, Rikako
Kogo, Mikihiko
Matsuda, Akio
Nishimura, Riko
Yoneda, Toshiyuki
author_facet Amano, Katsuhiko
Hata, Kenji
Muramatsu, Shuji
Wakabayashi, Makoto
Takigawa, Yoko
Ono, Koichiro
Nakanishi, Masako
Takashima, Rikako
Kogo, Mikihiko
Matsuda, Akio
Nishimura, Riko
Yoneda, Toshiyuki
author_sort Amano, Katsuhiko
collection PubMed
description SRY-box–containing gene 9 (Sox9) is an essential transcription factor in chondrocyte lineage determination and differentiation. Recent studies demonstrated that Sox9 controls the transcription of chondrocyte-specific genes in association with several other transcriptional regulators. To further understand the molecular mechanisms by which Sox9 influences transcriptional events during chondrocyte differentiation, we attempted to identify transcriptional partners of Sox9 and to examine their roles in chondrocyte differentiation. We isolated AT-rich interactive domain–containing protein 5a (Arid5a; also known as Mrf1) as an activator of the Col2a1 gene promoter from an ATDC5 cDNA library. Arid5a was highly expressed in cartilage and induced during chondrocyte differentiation. Furthermore, Arid5a physically interacted with Sox9 in nuclei and up-regulated the chondrocyte-specific action of Sox9. Overexpression of Arid5a stimulated chondrocyte differentiation in vitro and in an organ culture system. In contrast, Arid5a knockdown inhibited Col2a1 expression in chondrocytes. In addition, Arid5a binds directly to the promoter region of the Col2a1 gene and stimulates acetylation of histone 3 in the region. Our results suggest that Arid5a may directly interact with Sox9 and thereby enhance its chondrocyte-specific action.
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spelling pubmed-30780732011-06-30 Arid5a cooperates with Sox9 to stimulate chondrocyte-specific transcription Amano, Katsuhiko Hata, Kenji Muramatsu, Shuji Wakabayashi, Makoto Takigawa, Yoko Ono, Koichiro Nakanishi, Masako Takashima, Rikako Kogo, Mikihiko Matsuda, Akio Nishimura, Riko Yoneda, Toshiyuki Mol Biol Cell Articles SRY-box–containing gene 9 (Sox9) is an essential transcription factor in chondrocyte lineage determination and differentiation. Recent studies demonstrated that Sox9 controls the transcription of chondrocyte-specific genes in association with several other transcriptional regulators. To further understand the molecular mechanisms by which Sox9 influences transcriptional events during chondrocyte differentiation, we attempted to identify transcriptional partners of Sox9 and to examine their roles in chondrocyte differentiation. We isolated AT-rich interactive domain–containing protein 5a (Arid5a; also known as Mrf1) as an activator of the Col2a1 gene promoter from an ATDC5 cDNA library. Arid5a was highly expressed in cartilage and induced during chondrocyte differentiation. Furthermore, Arid5a physically interacted with Sox9 in nuclei and up-regulated the chondrocyte-specific action of Sox9. Overexpression of Arid5a stimulated chondrocyte differentiation in vitro and in an organ culture system. In contrast, Arid5a knockdown inhibited Col2a1 expression in chondrocytes. In addition, Arid5a binds directly to the promoter region of the Col2a1 gene and stimulates acetylation of histone 3 in the region. Our results suggest that Arid5a may directly interact with Sox9 and thereby enhance its chondrocyte-specific action. The American Society for Cell Biology 2011-04-15 /pmc/articles/PMC3078073/ /pubmed/21346191 http://dx.doi.org/10.1091/mbc.E10-07-0566 Text en © 2011 Amano et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0). “ASCB®,“ “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society of Cell Biology.
spellingShingle Articles
Amano, Katsuhiko
Hata, Kenji
Muramatsu, Shuji
Wakabayashi, Makoto
Takigawa, Yoko
Ono, Koichiro
Nakanishi, Masako
Takashima, Rikako
Kogo, Mikihiko
Matsuda, Akio
Nishimura, Riko
Yoneda, Toshiyuki
Arid5a cooperates with Sox9 to stimulate chondrocyte-specific transcription
title Arid5a cooperates with Sox9 to stimulate chondrocyte-specific transcription
title_full Arid5a cooperates with Sox9 to stimulate chondrocyte-specific transcription
title_fullStr Arid5a cooperates with Sox9 to stimulate chondrocyte-specific transcription
title_full_unstemmed Arid5a cooperates with Sox9 to stimulate chondrocyte-specific transcription
title_short Arid5a cooperates with Sox9 to stimulate chondrocyte-specific transcription
title_sort arid5a cooperates with sox9 to stimulate chondrocyte-specific transcription
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3078073/
https://www.ncbi.nlm.nih.gov/pubmed/21346191
http://dx.doi.org/10.1091/mbc.E10-07-0566
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