Cargando…
Mitotic centromeric targeting of HP1 and its binding to Sgo1 are dispensable for sister-chromatid cohesion in human cells
Human Shugoshin 1 (Sgo1) protects centromeric sister-chromatid cohesion during prophase and prevents premature sister-chromatid separation. Heterochromatin protein 1 (HP1) has been proposed to protect centromeric sister-chromatid cohesion by directly targeting Sgo1 to centromeres in mitosis. Here we...
Autores principales: | , , , , , |
---|---|
Formato: | Texto |
Lenguaje: | English |
Publicado: |
The American Society for Cell Biology
2011
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3078076/ https://www.ncbi.nlm.nih.gov/pubmed/21346195 http://dx.doi.org/10.1091/mbc.E11-01-0009 |
_version_ | 1782201908641923072 |
---|---|
author | Kang, Jungseog Chaudhary, Jaideep Dong, Hui Kim, Soonjoung Brautigam, Chad A. Yu, Hongtao |
author_facet | Kang, Jungseog Chaudhary, Jaideep Dong, Hui Kim, Soonjoung Brautigam, Chad A. Yu, Hongtao |
author_sort | Kang, Jungseog |
collection | PubMed |
description | Human Shugoshin 1 (Sgo1) protects centromeric sister-chromatid cohesion during prophase and prevents premature sister-chromatid separation. Heterochromatin protein 1 (HP1) has been proposed to protect centromeric sister-chromatid cohesion by directly targeting Sgo1 to centromeres in mitosis. Here we show that HP1α is targeted to mitotic centromeres by INCENP, a subunit of the chromosome passenger complex (CPC). Biochemical and structural studies show that both HP1–INCENP and HP1–Sgo1 interactions require the binding of the HP1 chromo shadow domain to PXVXL/I motifs in INCENP or Sgo1, suggesting that the INCENP-bound, centromeric HP1α is incapable of recruiting Sgo1. Consistently, a Sgo1 mutant deficient in HP1 binding is functional in centromeric cohesion protection and localizes normally to centromeres in mitosis. By contrast, INCENP or Sgo1 mutants deficient in HP1 binding fail to localize to centromeres in interphase. Therefore, our results suggest that HP1 binding by INCENP or Sgo1 is dispensable for centromeric cohesion protection during mitosis of human cells, but might regulate yet uncharacterized interphase functions of CPC or Sgo1 at the centromeres. |
format | Text |
id | pubmed-3078076 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | The American Society for Cell Biology |
record_format | MEDLINE/PubMed |
spelling | pubmed-30780762011-06-30 Mitotic centromeric targeting of HP1 and its binding to Sgo1 are dispensable for sister-chromatid cohesion in human cells Kang, Jungseog Chaudhary, Jaideep Dong, Hui Kim, Soonjoung Brautigam, Chad A. Yu, Hongtao Mol Biol Cell Articles Human Shugoshin 1 (Sgo1) protects centromeric sister-chromatid cohesion during prophase and prevents premature sister-chromatid separation. Heterochromatin protein 1 (HP1) has been proposed to protect centromeric sister-chromatid cohesion by directly targeting Sgo1 to centromeres in mitosis. Here we show that HP1α is targeted to mitotic centromeres by INCENP, a subunit of the chromosome passenger complex (CPC). Biochemical and structural studies show that both HP1–INCENP and HP1–Sgo1 interactions require the binding of the HP1 chromo shadow domain to PXVXL/I motifs in INCENP or Sgo1, suggesting that the INCENP-bound, centromeric HP1α is incapable of recruiting Sgo1. Consistently, a Sgo1 mutant deficient in HP1 binding is functional in centromeric cohesion protection and localizes normally to centromeres in mitosis. By contrast, INCENP or Sgo1 mutants deficient in HP1 binding fail to localize to centromeres in interphase. Therefore, our results suggest that HP1 binding by INCENP or Sgo1 is dispensable for centromeric cohesion protection during mitosis of human cells, but might regulate yet uncharacterized interphase functions of CPC or Sgo1 at the centromeres. The American Society for Cell Biology 2011-04-15 /pmc/articles/PMC3078076/ /pubmed/21346195 http://dx.doi.org/10.1091/mbc.E11-01-0009 Text en © 2011 Hongtao et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0). “ASCB®,“ “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society of Cell Biology. |
spellingShingle | Articles Kang, Jungseog Chaudhary, Jaideep Dong, Hui Kim, Soonjoung Brautigam, Chad A. Yu, Hongtao Mitotic centromeric targeting of HP1 and its binding to Sgo1 are dispensable for sister-chromatid cohesion in human cells |
title | Mitotic centromeric targeting of HP1 and its binding to Sgo1 are dispensable for sister-chromatid cohesion in human cells |
title_full | Mitotic centromeric targeting of HP1 and its binding to Sgo1 are dispensable for sister-chromatid cohesion in human cells |
title_fullStr | Mitotic centromeric targeting of HP1 and its binding to Sgo1 are dispensable for sister-chromatid cohesion in human cells |
title_full_unstemmed | Mitotic centromeric targeting of HP1 and its binding to Sgo1 are dispensable for sister-chromatid cohesion in human cells |
title_short | Mitotic centromeric targeting of HP1 and its binding to Sgo1 are dispensable for sister-chromatid cohesion in human cells |
title_sort | mitotic centromeric targeting of hp1 and its binding to sgo1 are dispensable for sister-chromatid cohesion in human cells |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3078076/ https://www.ncbi.nlm.nih.gov/pubmed/21346195 http://dx.doi.org/10.1091/mbc.E11-01-0009 |
work_keys_str_mv | AT kangjungseog mitoticcentromerictargetingofhp1anditsbindingtosgo1aredispensableforsisterchromatidcohesioninhumancells AT chaudharyjaideep mitoticcentromerictargetingofhp1anditsbindingtosgo1aredispensableforsisterchromatidcohesioninhumancells AT donghui mitoticcentromerictargetingofhp1anditsbindingtosgo1aredispensableforsisterchromatidcohesioninhumancells AT kimsoonjoung mitoticcentromerictargetingofhp1anditsbindingtosgo1aredispensableforsisterchromatidcohesioninhumancells AT brautigamchada mitoticcentromerictargetingofhp1anditsbindingtosgo1aredispensableforsisterchromatidcohesioninhumancells AT yuhongtao mitoticcentromerictargetingofhp1anditsbindingtosgo1aredispensableforsisterchromatidcohesioninhumancells |