Cargando…

Genetic variation of Mycobacterium tuberculosis circulating in Kharkiv Oblast, Ukraine

BACKGROUND: A persistent increase of tuberculosis cases has recently been noted in the Ukraine. The reported incidence of drug-resistant isolates of M. tuberculosis is growing steadily; however, data on the genetic variation of isolates of M. tuberculosis circulating in northern Ukraine and on the s...

Descripción completa

Detalles Bibliográficos
Autores principales: Dymova, Maya A, Liashenko, Oleksander O, Poteiko, Petro I, Krutko, Valeriy S, Khrapov, Eugeny A, Filipenko, Maxim L
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3079650/
https://www.ncbi.nlm.nih.gov/pubmed/21439097
http://dx.doi.org/10.1186/1471-2334-11-77
_version_ 1782202037003354112
author Dymova, Maya A
Liashenko, Oleksander O
Poteiko, Petro I
Krutko, Valeriy S
Khrapov, Eugeny A
Filipenko, Maxim L
author_facet Dymova, Maya A
Liashenko, Oleksander O
Poteiko, Petro I
Krutko, Valeriy S
Khrapov, Eugeny A
Filipenko, Maxim L
author_sort Dymova, Maya A
collection PubMed
description BACKGROUND: A persistent increase of tuberculosis cases has recently been noted in the Ukraine. The reported incidence of drug-resistant isolates of M. tuberculosis is growing steadily; however, data on the genetic variation of isolates of M. tuberculosis circulating in northern Ukraine and on the spectrum and frequency of occurrence of mutations determining resistance to the principal anti-tuberculosis drugs isoniazid and rifampicin have not yet been reported. METHODS: Isolates of M. tuberculosis from 98 tuberculosis patients living in Kharkiv Oblast (Ukraine) were analyzed using VNTR- and RFLP-IS6110-typing methods. Mutations associated with resistance to rifampicin and isoniazid were detected by RFLP-PCR methods, and also confirmed by sequencing. RESULTS: We identified 75 different genetic profiles. Thirty four (34%) isolates belonged to the Beijing genotype and 23 (23%) isolates belonged to the LAM family. A cluster of isolates belonging to the LAM family had significant genetic heterogeneity, indicating that this family had an ancient distribution and circulation in this geographical region. Moreover, we found a significant percentage of the isolates (36%) belonged to as yet unidentified families of M. tuberculosis or had individual non-clustering genotypes. Mutations conferring rifampicin and isoniazid resistance were detected in 49% and 54% isolates, respectively. Mutations in codon 531 of the rpoB gene and codon 315 of the katG gene were predominant among drug-resistant isolates. An association was found for belonging to the LAM strain family and having multiple drug resistance (R = 0.27, p = 0.0059) and also for the presence of a mutation in codon 531 of the rpoB gene and belonging to the Beijing strain family (R = 0.2, p = 0.04). CONCLUSIONS: Transmission of drug-resistant isolates seems to contribute to the spread of resistant TB in this oblast. The Beijing genotype and LAM genotype should be seen as a major cause of drug resistant TB in this region.
format Text
id pubmed-3079650
institution National Center for Biotechnology Information
language English
publishDate 2011
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-30796502011-04-20 Genetic variation of Mycobacterium tuberculosis circulating in Kharkiv Oblast, Ukraine Dymova, Maya A Liashenko, Oleksander O Poteiko, Petro I Krutko, Valeriy S Khrapov, Eugeny A Filipenko, Maxim L BMC Infect Dis Research Article BACKGROUND: A persistent increase of tuberculosis cases has recently been noted in the Ukraine. The reported incidence of drug-resistant isolates of M. tuberculosis is growing steadily; however, data on the genetic variation of isolates of M. tuberculosis circulating in northern Ukraine and on the spectrum and frequency of occurrence of mutations determining resistance to the principal anti-tuberculosis drugs isoniazid and rifampicin have not yet been reported. METHODS: Isolates of M. tuberculosis from 98 tuberculosis patients living in Kharkiv Oblast (Ukraine) were analyzed using VNTR- and RFLP-IS6110-typing methods. Mutations associated with resistance to rifampicin and isoniazid were detected by RFLP-PCR methods, and also confirmed by sequencing. RESULTS: We identified 75 different genetic profiles. Thirty four (34%) isolates belonged to the Beijing genotype and 23 (23%) isolates belonged to the LAM family. A cluster of isolates belonging to the LAM family had significant genetic heterogeneity, indicating that this family had an ancient distribution and circulation in this geographical region. Moreover, we found a significant percentage of the isolates (36%) belonged to as yet unidentified families of M. tuberculosis or had individual non-clustering genotypes. Mutations conferring rifampicin and isoniazid resistance were detected in 49% and 54% isolates, respectively. Mutations in codon 531 of the rpoB gene and codon 315 of the katG gene were predominant among drug-resistant isolates. An association was found for belonging to the LAM strain family and having multiple drug resistance (R = 0.27, p = 0.0059) and also for the presence of a mutation in codon 531 of the rpoB gene and belonging to the Beijing strain family (R = 0.2, p = 0.04). CONCLUSIONS: Transmission of drug-resistant isolates seems to contribute to the spread of resistant TB in this oblast. The Beijing genotype and LAM genotype should be seen as a major cause of drug resistant TB in this region. BioMed Central 2011-03-28 /pmc/articles/PMC3079650/ /pubmed/21439097 http://dx.doi.org/10.1186/1471-2334-11-77 Text en Copyright ©2011 Dymova et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Dymova, Maya A
Liashenko, Oleksander O
Poteiko, Petro I
Krutko, Valeriy S
Khrapov, Eugeny A
Filipenko, Maxim L
Genetic variation of Mycobacterium tuberculosis circulating in Kharkiv Oblast, Ukraine
title Genetic variation of Mycobacterium tuberculosis circulating in Kharkiv Oblast, Ukraine
title_full Genetic variation of Mycobacterium tuberculosis circulating in Kharkiv Oblast, Ukraine
title_fullStr Genetic variation of Mycobacterium tuberculosis circulating in Kharkiv Oblast, Ukraine
title_full_unstemmed Genetic variation of Mycobacterium tuberculosis circulating in Kharkiv Oblast, Ukraine
title_short Genetic variation of Mycobacterium tuberculosis circulating in Kharkiv Oblast, Ukraine
title_sort genetic variation of mycobacterium tuberculosis circulating in kharkiv oblast, ukraine
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3079650/
https://www.ncbi.nlm.nih.gov/pubmed/21439097
http://dx.doi.org/10.1186/1471-2334-11-77
work_keys_str_mv AT dymovamayaa geneticvariationofmycobacteriumtuberculosiscirculatinginkharkivoblastukraine
AT liashenkooleksandero geneticvariationofmycobacteriumtuberculosiscirculatinginkharkivoblastukraine
AT poteikopetroi geneticvariationofmycobacteriumtuberculosiscirculatinginkharkivoblastukraine
AT krutkovaleriys geneticvariationofmycobacteriumtuberculosiscirculatinginkharkivoblastukraine
AT khrapoveugenya geneticvariationofmycobacteriumtuberculosiscirculatinginkharkivoblastukraine
AT filipenkomaximl geneticvariationofmycobacteriumtuberculosiscirculatinginkharkivoblastukraine