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Chromosome passenger complexes control anaphase duration and spindle elongation via a kinesin-5 brake

During mitosis, chromosome passenger complexes (CPCs) exhibit a well-conserved association with the anaphase spindle and have been implicated in spindle stability. However, their precise effect on the spindle is not clear. In this paper, we show, in budding yeast, that a CPC consisting of CBF3, Bir1...

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Detalles Bibliográficos
Autores principales: Rozelle, Daniel K., Hansen, Scott D., Kaplan, Kenneth B.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3080259/
https://www.ncbi.nlm.nih.gov/pubmed/21482719
http://dx.doi.org/10.1083/jcb.201011002
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author Rozelle, Daniel K.
Hansen, Scott D.
Kaplan, Kenneth B.
author_facet Rozelle, Daniel K.
Hansen, Scott D.
Kaplan, Kenneth B.
author_sort Rozelle, Daniel K.
collection PubMed
description During mitosis, chromosome passenger complexes (CPCs) exhibit a well-conserved association with the anaphase spindle and have been implicated in spindle stability. However, their precise effect on the spindle is not clear. In this paper, we show, in budding yeast, that a CPC consisting of CBF3, Bir1, and Sli15, but not Ipl1, is required for normal spindle elongation. CPC mutants slow spindle elongation through the action of the bipolar kinesins Cin8 and Kip1. The same CPC mutants that slow spindle elongation also result in the enrichment of Cin8 and Kip1 at the spindle midzone. Together, these findings argue that CPCs function to organize the spindle midzone and potentially switch motors between force generators and molecular brakes. We also find that slowing spindle elongation delays the mitotic exit network (MEN)–dependent release of Cdc14, thus delaying spindle breakdown until a minimal spindle size is reached. We propose that these CPC- and MEN-dependent mechanisms are important for coordinating chromosome segregation with spindle breakdown and mitotic exit.
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spelling pubmed-30802592011-10-18 Chromosome passenger complexes control anaphase duration and spindle elongation via a kinesin-5 brake Rozelle, Daniel K. Hansen, Scott D. Kaplan, Kenneth B. J Cell Biol Research Articles During mitosis, chromosome passenger complexes (CPCs) exhibit a well-conserved association with the anaphase spindle and have been implicated in spindle stability. However, their precise effect on the spindle is not clear. In this paper, we show, in budding yeast, that a CPC consisting of CBF3, Bir1, and Sli15, but not Ipl1, is required for normal spindle elongation. CPC mutants slow spindle elongation through the action of the bipolar kinesins Cin8 and Kip1. The same CPC mutants that slow spindle elongation also result in the enrichment of Cin8 and Kip1 at the spindle midzone. Together, these findings argue that CPCs function to organize the spindle midzone and potentially switch motors between force generators and molecular brakes. We also find that slowing spindle elongation delays the mitotic exit network (MEN)–dependent release of Cdc14, thus delaying spindle breakdown until a minimal spindle size is reached. We propose that these CPC- and MEN-dependent mechanisms are important for coordinating chromosome segregation with spindle breakdown and mitotic exit. The Rockefeller University Press 2011-04-18 /pmc/articles/PMC3080259/ /pubmed/21482719 http://dx.doi.org/10.1083/jcb.201011002 Text en © 2011 Rozelle et al. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).
spellingShingle Research Articles
Rozelle, Daniel K.
Hansen, Scott D.
Kaplan, Kenneth B.
Chromosome passenger complexes control anaphase duration and spindle elongation via a kinesin-5 brake
title Chromosome passenger complexes control anaphase duration and spindle elongation via a kinesin-5 brake
title_full Chromosome passenger complexes control anaphase duration and spindle elongation via a kinesin-5 brake
title_fullStr Chromosome passenger complexes control anaphase duration and spindle elongation via a kinesin-5 brake
title_full_unstemmed Chromosome passenger complexes control anaphase duration and spindle elongation via a kinesin-5 brake
title_short Chromosome passenger complexes control anaphase duration and spindle elongation via a kinesin-5 brake
title_sort chromosome passenger complexes control anaphase duration and spindle elongation via a kinesin-5 brake
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3080259/
https://www.ncbi.nlm.nih.gov/pubmed/21482719
http://dx.doi.org/10.1083/jcb.201011002
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