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Oligomerization of the E. coli Core RNA Polymerase: Formation of (α(2)ββ'ω)(2)–DNA Complexes and Regulation of the Oligomerization by Auxiliary Subunits

In this work, using multiple, dissimilar physico-chemical techniques, we demonstrate that the Escherichia coli RNA polymerase core enzyme obtained through a classic purification procedure forms stable (α(2)ββ'ω)(2) complexes in the presence or absence of short DNA probes. Multiple control exper...

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Detalles Bibliográficos
Autores principales: Kansara, Seema G., Sukhodolets, Maxim V.
Formato: Texto
Lenguaje:English
Publicado: Public Library of Science 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3080401/
https://www.ncbi.nlm.nih.gov/pubmed/21533049
http://dx.doi.org/10.1371/journal.pone.0018990