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Characterization of n-Hexane sub-fraction of Bridelia micrantha (Berth) and its antimycobacterium activity
BACKGROUND: Tuberculosis, caused by Mycobacterium tuberculosis (MTB), is the most notified disease in the world. Development of resistance to first line drugs by MTB is a public health concern. As a result, there is the search for new and novel sources of antimycobacterial drugs for example from med...
Autores principales: | , , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3080840/ https://www.ncbi.nlm.nih.gov/pubmed/21481267 http://dx.doi.org/10.1186/1472-6882-11-28 |
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author | Green, Ezekiel Obi, Lawrence C Samie, Amidou Bessong, Pascal O Ndip, Roland N |
author_facet | Green, Ezekiel Obi, Lawrence C Samie, Amidou Bessong, Pascal O Ndip, Roland N |
author_sort | Green, Ezekiel |
collection | PubMed |
description | BACKGROUND: Tuberculosis, caused by Mycobacterium tuberculosis (MTB), is the most notified disease in the world. Development of resistance to first line drugs by MTB is a public health concern. As a result, there is the search for new and novel sources of antimycobacterial drugs for example from medicinal plants. In this study we determined the in vitro antimycobacterial activity of n-Hexane sub-fraction from Bridelia micrantha (Berth) against MTB H(37)Ra and a clinical isolate resistant to all five first-line antituberculosis drugs. METHODS: The antimycobacterial activity of the n-Hexane sub-fraction of ethyl acetate fractions from acetone extracts of B. micrantha barks was evaluated using the resazurin microplate assay against two MTB isolates. Bioassay-guided fractionation of the ethyl acetate fraction was performed using 100% n-Hexane and Chloroform/Methanol (99:1) as solvents in order of increasing polarity by column chromatography and Resazurin microtiter plate assay for susceptibility tests. RESULTS: The n-Hexane fraction showed 20% inhibition of MTB H(37)Ra and almost 35% inhibition of an MTB isolate resistant to all first-line drugs at 10 μg/mL. GC/MS analysis of the fraction resulted in the identification of twenty-four constituents representing 60.5% of the fraction. Some of the 24 compounds detected included Benzene, 1.3-bis (3-phenoxyphenoxy (13.51%), 2-pinen-4-one (10.03%), N(b)-benzyl-14-(carboxymethyl) (6.35%) and the least detected compound was linalool (0.2%). CONCLUSIONS: The results show that the n-Hexane fraction of B. micrantha has antimycobacterial activity. |
format | Text |
id | pubmed-3080840 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-30808402011-04-22 Characterization of n-Hexane sub-fraction of Bridelia micrantha (Berth) and its antimycobacterium activity Green, Ezekiel Obi, Lawrence C Samie, Amidou Bessong, Pascal O Ndip, Roland N BMC Complement Altern Med Research Article BACKGROUND: Tuberculosis, caused by Mycobacterium tuberculosis (MTB), is the most notified disease in the world. Development of resistance to first line drugs by MTB is a public health concern. As a result, there is the search for new and novel sources of antimycobacterial drugs for example from medicinal plants. In this study we determined the in vitro antimycobacterial activity of n-Hexane sub-fraction from Bridelia micrantha (Berth) against MTB H(37)Ra and a clinical isolate resistant to all five first-line antituberculosis drugs. METHODS: The antimycobacterial activity of the n-Hexane sub-fraction of ethyl acetate fractions from acetone extracts of B. micrantha barks was evaluated using the resazurin microplate assay against two MTB isolates. Bioassay-guided fractionation of the ethyl acetate fraction was performed using 100% n-Hexane and Chloroform/Methanol (99:1) as solvents in order of increasing polarity by column chromatography and Resazurin microtiter plate assay for susceptibility tests. RESULTS: The n-Hexane fraction showed 20% inhibition of MTB H(37)Ra and almost 35% inhibition of an MTB isolate resistant to all first-line drugs at 10 μg/mL. GC/MS analysis of the fraction resulted in the identification of twenty-four constituents representing 60.5% of the fraction. Some of the 24 compounds detected included Benzene, 1.3-bis (3-phenoxyphenoxy (13.51%), 2-pinen-4-one (10.03%), N(b)-benzyl-14-(carboxymethyl) (6.35%) and the least detected compound was linalool (0.2%). CONCLUSIONS: The results show that the n-Hexane fraction of B. micrantha has antimycobacterial activity. BioMed Central 2011-04-11 /pmc/articles/PMC3080840/ /pubmed/21481267 http://dx.doi.org/10.1186/1472-6882-11-28 Text en Copyright ©2011 Green et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Green, Ezekiel Obi, Lawrence C Samie, Amidou Bessong, Pascal O Ndip, Roland N Characterization of n-Hexane sub-fraction of Bridelia micrantha (Berth) and its antimycobacterium activity |
title | Characterization of n-Hexane sub-fraction of Bridelia micrantha (Berth) and its antimycobacterium activity |
title_full | Characterization of n-Hexane sub-fraction of Bridelia micrantha (Berth) and its antimycobacterium activity |
title_fullStr | Characterization of n-Hexane sub-fraction of Bridelia micrantha (Berth) and its antimycobacterium activity |
title_full_unstemmed | Characterization of n-Hexane sub-fraction of Bridelia micrantha (Berth) and its antimycobacterium activity |
title_short | Characterization of n-Hexane sub-fraction of Bridelia micrantha (Berth) and its antimycobacterium activity |
title_sort | characterization of n-hexane sub-fraction of bridelia micrantha (berth) and its antimycobacterium activity |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3080840/ https://www.ncbi.nlm.nih.gov/pubmed/21481267 http://dx.doi.org/10.1186/1472-6882-11-28 |
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