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Multiple Changes in Peptide and Lipid Expression Associated with Regeneration in the Nervous System of the Medicinal Leech
BACKGROUND: The adult medicinal leech central nervous system (CNS) is capable of regenerating specific synaptic circuitry after a mechanical lesion, displaying evidence of anatomical repair within a few days and functional recovery within a few weeks. In the present work, spatiotemporal changes in m...
Autores principales: | , , , , , , , , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3081291/ https://www.ncbi.nlm.nih.gov/pubmed/21526169 http://dx.doi.org/10.1371/journal.pone.0018359 |
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author | Meriaux, Céline Arafah, Karim Tasiemski, Aurélie Wisztorski, Maxence Bruand, Jocelyne Boidin-Wichlacz, Céline Desmons, Annie Debois, Delphine Laprévote, Olivier Brunelle, Alain Gaasterland, Terry Macagno, Eduardo Fournier, Isabelle Salzet, Michel |
author_facet | Meriaux, Céline Arafah, Karim Tasiemski, Aurélie Wisztorski, Maxence Bruand, Jocelyne Boidin-Wichlacz, Céline Desmons, Annie Debois, Delphine Laprévote, Olivier Brunelle, Alain Gaasterland, Terry Macagno, Eduardo Fournier, Isabelle Salzet, Michel |
author_sort | Meriaux, Céline |
collection | PubMed |
description | BACKGROUND: The adult medicinal leech central nervous system (CNS) is capable of regenerating specific synaptic circuitry after a mechanical lesion, displaying evidence of anatomical repair within a few days and functional recovery within a few weeks. In the present work, spatiotemporal changes in molecular distributions during this phenomenon are explored. Moreover, the hypothesis that neural regeneration involves some molecular factors initially employed during embryonic neural development is tested. RESULTS: Imaging mass spectrometry coupled to peptidomic and lipidomic methodologies allowed the selection of molecules whose spatiotemporal pattern of expression was of potential interest. The identification of peptides was aided by comparing MS/MS spectra obtained for the peptidome extracted from embryonic and adult tissues to leech transcriptome and genome databases. Through the parallel use of a classical lipidomic approach and secondary ion mass spectrometry, specific lipids, including cannabinoids, gangliosides and several other types, were detected in adult ganglia following mechanical damage to connected nerves. These observations motivated a search for possible effects of cannabinoids on neurite outgrowth. Exposing nervous tissues to Transient Receptor Potential Vanilloid (TRPV) receptor agonists resulted in enhanced neurite outgrowth from a cut nerve, while exposure to antagonists blocked such outgrowth. CONCLUSION: The experiments on the regenerating adult leech CNS reported here provide direct evidence of increased titers of proteins that are thought to play important roles in early stages of neural development. Our data further suggest that endocannabinoids also play key roles in CNS regeneration, mediated through the activation of leech TRPVs, as a thorough search of leech genome databases failed to reveal any leech orthologs of the mammalian cannabinoid receptors but revealed putative TRPVs. In sum, our observations identify a number of lipids and proteins that may contribute to different aspects of the complex phenomenon of leech nerve regeneration, establishing an important base for future functional assays. |
format | Text |
id | pubmed-3081291 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-30812912011-04-27 Multiple Changes in Peptide and Lipid Expression Associated with Regeneration in the Nervous System of the Medicinal Leech Meriaux, Céline Arafah, Karim Tasiemski, Aurélie Wisztorski, Maxence Bruand, Jocelyne Boidin-Wichlacz, Céline Desmons, Annie Debois, Delphine Laprévote, Olivier Brunelle, Alain Gaasterland, Terry Macagno, Eduardo Fournier, Isabelle Salzet, Michel PLoS One Research Article BACKGROUND: The adult medicinal leech central nervous system (CNS) is capable of regenerating specific synaptic circuitry after a mechanical lesion, displaying evidence of anatomical repair within a few days and functional recovery within a few weeks. In the present work, spatiotemporal changes in molecular distributions during this phenomenon are explored. Moreover, the hypothesis that neural regeneration involves some molecular factors initially employed during embryonic neural development is tested. RESULTS: Imaging mass spectrometry coupled to peptidomic and lipidomic methodologies allowed the selection of molecules whose spatiotemporal pattern of expression was of potential interest. The identification of peptides was aided by comparing MS/MS spectra obtained for the peptidome extracted from embryonic and adult tissues to leech transcriptome and genome databases. Through the parallel use of a classical lipidomic approach and secondary ion mass spectrometry, specific lipids, including cannabinoids, gangliosides and several other types, were detected in adult ganglia following mechanical damage to connected nerves. These observations motivated a search for possible effects of cannabinoids on neurite outgrowth. Exposing nervous tissues to Transient Receptor Potential Vanilloid (TRPV) receptor agonists resulted in enhanced neurite outgrowth from a cut nerve, while exposure to antagonists blocked such outgrowth. CONCLUSION: The experiments on the regenerating adult leech CNS reported here provide direct evidence of increased titers of proteins that are thought to play important roles in early stages of neural development. Our data further suggest that endocannabinoids also play key roles in CNS regeneration, mediated through the activation of leech TRPVs, as a thorough search of leech genome databases failed to reveal any leech orthologs of the mammalian cannabinoid receptors but revealed putative TRPVs. In sum, our observations identify a number of lipids and proteins that may contribute to different aspects of the complex phenomenon of leech nerve regeneration, establishing an important base for future functional assays. Public Library of Science 2011-04-22 /pmc/articles/PMC3081291/ /pubmed/21526169 http://dx.doi.org/10.1371/journal.pone.0018359 Text en Meriaux et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Meriaux, Céline Arafah, Karim Tasiemski, Aurélie Wisztorski, Maxence Bruand, Jocelyne Boidin-Wichlacz, Céline Desmons, Annie Debois, Delphine Laprévote, Olivier Brunelle, Alain Gaasterland, Terry Macagno, Eduardo Fournier, Isabelle Salzet, Michel Multiple Changes in Peptide and Lipid Expression Associated with Regeneration in the Nervous System of the Medicinal Leech |
title | Multiple Changes in Peptide and Lipid Expression Associated with Regeneration in the Nervous System of the Medicinal Leech |
title_full | Multiple Changes in Peptide and Lipid Expression Associated with Regeneration in the Nervous System of the Medicinal Leech |
title_fullStr | Multiple Changes in Peptide and Lipid Expression Associated with Regeneration in the Nervous System of the Medicinal Leech |
title_full_unstemmed | Multiple Changes in Peptide and Lipid Expression Associated with Regeneration in the Nervous System of the Medicinal Leech |
title_short | Multiple Changes in Peptide and Lipid Expression Associated with Regeneration in the Nervous System of the Medicinal Leech |
title_sort | multiple changes in peptide and lipid expression associated with regeneration in the nervous system of the medicinal leech |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3081291/ https://www.ncbi.nlm.nih.gov/pubmed/21526169 http://dx.doi.org/10.1371/journal.pone.0018359 |
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