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Development of a new marker system for identifying the complex members of the low-molecular-weight glutenin subunit gene family in bread wheat (Triticum aestivum L.)
Low-molecular-weight glutenin subunits (LMW-GSs) play an important role in determining the bread-making quality of bread wheat. However, LMW-GSs display high polymorphic protein complexes encoded by multiple genes, and elucidating the complex LMW-GS gene family in bread wheat remains challenging. In...
Autores principales: | , , , , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
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Springer-Verlag
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3082022/ https://www.ncbi.nlm.nih.gov/pubmed/21344181 http://dx.doi.org/10.1007/s00122-011-1550-7 |
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author | Zhang, Xiaofei Liu, Dongcheng Yang, Wenlong Liu, Kunfan Sun, Jiazhu Guo, Xiaoli Li, Yiwen Wang, Daowen Ling, Hongqing Zhang, Aimin |
author_facet | Zhang, Xiaofei Liu, Dongcheng Yang, Wenlong Liu, Kunfan Sun, Jiazhu Guo, Xiaoli Li, Yiwen Wang, Daowen Ling, Hongqing Zhang, Aimin |
author_sort | Zhang, Xiaofei |
collection | PubMed |
description | Low-molecular-weight glutenin subunits (LMW-GSs) play an important role in determining the bread-making quality of bread wheat. However, LMW-GSs display high polymorphic protein complexes encoded by multiple genes, and elucidating the complex LMW-GS gene family in bread wheat remains challenging. In the present study, using conventional polymerase chain reaction (PCR) with conserved primers and high-resolution capillary electrophoresis, we developed a new molecular marker system for identifying LMW-GS gene family members. Based on sequence alignment of 13 LMW-GS genes previously identified in the Chinese bread wheat variety Xiaoyan 54 and other genes available in GenBank, PCR primers were developed and assigned to conserved sequences spanning the length polymorphism regions of LMW-GS genes. After PCR amplification, 17 DNA fragments in Xiaoyan 54 were detected using capillary electrophoresis. In total, 13 fragments were identical to previously identified LMW-GS genes, and the other 4 were derived from unique LMW-GS genes by sequencing. This marker system was also used to identify LMW-GS genes in Chinese Spring and its group 1 nulli–tetrasomic lines. Among the 17 detected DNA fragments, 4 were located on chromosome 1A, 5 on 1B, and 8 on 1D. The results suggest that this marker system is useful for large-scale identification of LMW-GS genes in bread wheat varieties, and for the selection of desirable LMW-GS genes to improve the bread-making quality in wheat molecular breeding programmes. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s00122-011-1550-7) contains supplementary material, which is available to authorized users. |
format | Text |
id | pubmed-3082022 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | Springer-Verlag |
record_format | MEDLINE/PubMed |
spelling | pubmed-30820222011-06-06 Development of a new marker system for identifying the complex members of the low-molecular-weight glutenin subunit gene family in bread wheat (Triticum aestivum L.) Zhang, Xiaofei Liu, Dongcheng Yang, Wenlong Liu, Kunfan Sun, Jiazhu Guo, Xiaoli Li, Yiwen Wang, Daowen Ling, Hongqing Zhang, Aimin Theor Appl Genet Original Paper Low-molecular-weight glutenin subunits (LMW-GSs) play an important role in determining the bread-making quality of bread wheat. However, LMW-GSs display high polymorphic protein complexes encoded by multiple genes, and elucidating the complex LMW-GS gene family in bread wheat remains challenging. In the present study, using conventional polymerase chain reaction (PCR) with conserved primers and high-resolution capillary electrophoresis, we developed a new molecular marker system for identifying LMW-GS gene family members. Based on sequence alignment of 13 LMW-GS genes previously identified in the Chinese bread wheat variety Xiaoyan 54 and other genes available in GenBank, PCR primers were developed and assigned to conserved sequences spanning the length polymorphism regions of LMW-GS genes. After PCR amplification, 17 DNA fragments in Xiaoyan 54 were detected using capillary electrophoresis. In total, 13 fragments were identical to previously identified LMW-GS genes, and the other 4 were derived from unique LMW-GS genes by sequencing. This marker system was also used to identify LMW-GS genes in Chinese Spring and its group 1 nulli–tetrasomic lines. Among the 17 detected DNA fragments, 4 were located on chromosome 1A, 5 on 1B, and 8 on 1D. The results suggest that this marker system is useful for large-scale identification of LMW-GS genes in bread wheat varieties, and for the selection of desirable LMW-GS genes to improve the bread-making quality in wheat molecular breeding programmes. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s00122-011-1550-7) contains supplementary material, which is available to authorized users. Springer-Verlag 2011-02-23 2011 /pmc/articles/PMC3082022/ /pubmed/21344181 http://dx.doi.org/10.1007/s00122-011-1550-7 Text en © The Author(s) 2011 https://creativecommons.org/licenses/by-nc/4.0/This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and source are credited. |
spellingShingle | Original Paper Zhang, Xiaofei Liu, Dongcheng Yang, Wenlong Liu, Kunfan Sun, Jiazhu Guo, Xiaoli Li, Yiwen Wang, Daowen Ling, Hongqing Zhang, Aimin Development of a new marker system for identifying the complex members of the low-molecular-weight glutenin subunit gene family in bread wheat (Triticum aestivum L.) |
title | Development of a new marker system for identifying the complex members of the low-molecular-weight glutenin subunit gene family in bread wheat (Triticum aestivum L.) |
title_full | Development of a new marker system for identifying the complex members of the low-molecular-weight glutenin subunit gene family in bread wheat (Triticum aestivum L.) |
title_fullStr | Development of a new marker system for identifying the complex members of the low-molecular-weight glutenin subunit gene family in bread wheat (Triticum aestivum L.) |
title_full_unstemmed | Development of a new marker system for identifying the complex members of the low-molecular-weight glutenin subunit gene family in bread wheat (Triticum aestivum L.) |
title_short | Development of a new marker system for identifying the complex members of the low-molecular-weight glutenin subunit gene family in bread wheat (Triticum aestivum L.) |
title_sort | development of a new marker system for identifying the complex members of the low-molecular-weight glutenin subunit gene family in bread wheat (triticum aestivum l.) |
topic | Original Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3082022/ https://www.ncbi.nlm.nih.gov/pubmed/21344181 http://dx.doi.org/10.1007/s00122-011-1550-7 |
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