Selection of silk-binding peptides by phage display

Peptides that bind to silkworm-derived silk fibroin fiber were selected from a phage-displayed random peptide library. The selected silk-binding peptides contained a consensus sequence QSWS which is important for silk-binding as confirmed by binding assays using phage and synthetic peptides. With fu...

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Detalles Bibliográficos
Autores principales: Nomura, Yoko, Sharma, Vandana, Yamamura, Asami, Yokobayashi, Yohei
Formato: Texto
Lenguaje:English
Publicado: Springer Netherlands 2011
Materias:
Original Research Paper
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3082026/
https://www.ncbi.nlm.nih.gov/pubmed/21259034
http://dx.doi.org/10.1007/s10529-011-0519-6
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author Nomura, Yoko
Sharma, Vandana
Yamamura, Asami
Yokobayashi, Yohei
author_facet Nomura, Yoko
Sharma, Vandana
Yamamura, Asami
Yokobayashi, Yohei
author_sort Nomura, Yoko
collection PubMed
description Peptides that bind to silkworm-derived silk fibroin fiber were selected from a phage-displayed random peptide library. The selected silk-binding peptides contained a consensus sequence QSWS which is important for silk-binding as confirmed by binding assays using phage and synthetic peptides. With further optimization, we anticipate that the silk-binding peptides will be useful for functionalization of silk for biomaterial applications.
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spelling pubmed-30820262011-06-06 Selection of silk-binding peptides by phage display Nomura, Yoko Sharma, Vandana Yamamura, Asami Yokobayashi, Yohei Biotechnol Lett Original Research Paper Peptides that bind to silkworm-derived silk fibroin fiber were selected from a phage-displayed random peptide library. The selected silk-binding peptides contained a consensus sequence QSWS which is important for silk-binding as confirmed by binding assays using phage and synthetic peptides. With further optimization, we anticipate that the silk-binding peptides will be useful for functionalization of silk for biomaterial applications. Springer Netherlands 2011-01-23 2011 /pmc/articles/PMC3082026/ /pubmed/21259034 http://dx.doi.org/10.1007/s10529-011-0519-6 Text en © The Author(s) 2011 https://creativecommons.org/licenses/by-nc/4.0/This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and source are credited.
spellingShingle Original Research Paper
Nomura, Yoko
Sharma, Vandana
Yamamura, Asami
Yokobayashi, Yohei
Selection of silk-binding peptides by phage display
title Selection of silk-binding peptides by phage display
title_full Selection of silk-binding peptides by phage display
title_fullStr Selection of silk-binding peptides by phage display
title_full_unstemmed Selection of silk-binding peptides by phage display
title_short Selection of silk-binding peptides by phage display
title_sort selection of silk-binding peptides by phage display
topic Original Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3082026/
https://www.ncbi.nlm.nih.gov/pubmed/21259034
http://dx.doi.org/10.1007/s10529-011-0519-6
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