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HP1α recruitment to DNA damage by p150CAF-1 promotes homologous recombination repair

Heterochromatin protein 1 (HP1), a major component of constitutive heterochromatin, is recruited to DNA damage sites. However, the mechanism involved in this recruitment and its functional importance during DNA repair remain major unresolved issues. Here, by characterizing HP1α dynamics at laser-ind...

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Detalles Bibliográficos
Autores principales: Baldeyron, Céline, Soria, Gaston, Roche, Danièle, Cook, Adam J. L., Almouzni, Geneviève
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3082177/
https://www.ncbi.nlm.nih.gov/pubmed/21464229
http://dx.doi.org/10.1083/jcb.201101030
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author Baldeyron, Céline
Soria, Gaston
Roche, Danièle
Cook, Adam J. L.
Almouzni, Geneviève
author_facet Baldeyron, Céline
Soria, Gaston
Roche, Danièle
Cook, Adam J. L.
Almouzni, Geneviève
author_sort Baldeyron, Céline
collection PubMed
description Heterochromatin protein 1 (HP1), a major component of constitutive heterochromatin, is recruited to DNA damage sites. However, the mechanism involved in this recruitment and its functional importance during DNA repair remain major unresolved issues. Here, by characterizing HP1α dynamics at laser-induced damage sites in mammalian cells, we show that the de novo accumulation of HP1α occurs within both euchromatin and heterochromatin as a rapid and transient event after DNA damage. This recruitment is strictly dependent on p150CAF-1, the largest subunit of chromatin assembly factor 1 (CAF-1), and its ability to interact with HP1α. We find that HP1α depletion severely compromises the recruitment of the DNA damage response (DDR) proteins 53BP1 and RAD51. Moreover, HP1α depletion leads to defects in homologous recombination–mediated repair and reduces cell survival after DNA damage. Collectively, our data reveal that HP1α recruitment at early stages of the DDR involves p150CAF-1 and is critical for proper DNA damage signaling and repair.
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spelling pubmed-30821772011-10-04 HP1α recruitment to DNA damage by p150CAF-1 promotes homologous recombination repair Baldeyron, Céline Soria, Gaston Roche, Danièle Cook, Adam J. L. Almouzni, Geneviève J Cell Biol Research Articles Heterochromatin protein 1 (HP1), a major component of constitutive heterochromatin, is recruited to DNA damage sites. However, the mechanism involved in this recruitment and its functional importance during DNA repair remain major unresolved issues. Here, by characterizing HP1α dynamics at laser-induced damage sites in mammalian cells, we show that the de novo accumulation of HP1α occurs within both euchromatin and heterochromatin as a rapid and transient event after DNA damage. This recruitment is strictly dependent on p150CAF-1, the largest subunit of chromatin assembly factor 1 (CAF-1), and its ability to interact with HP1α. We find that HP1α depletion severely compromises the recruitment of the DNA damage response (DDR) proteins 53BP1 and RAD51. Moreover, HP1α depletion leads to defects in homologous recombination–mediated repair and reduces cell survival after DNA damage. Collectively, our data reveal that HP1α recruitment at early stages of the DDR involves p150CAF-1 and is critical for proper DNA damage signaling and repair. The Rockefeller University Press 2011-04-04 /pmc/articles/PMC3082177/ /pubmed/21464229 http://dx.doi.org/10.1083/jcb.201101030 Text en © 2011 Baldeyron et al. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).
spellingShingle Research Articles
Baldeyron, Céline
Soria, Gaston
Roche, Danièle
Cook, Adam J. L.
Almouzni, Geneviève
HP1α recruitment to DNA damage by p150CAF-1 promotes homologous recombination repair
title HP1α recruitment to DNA damage by p150CAF-1 promotes homologous recombination repair
title_full HP1α recruitment to DNA damage by p150CAF-1 promotes homologous recombination repair
title_fullStr HP1α recruitment to DNA damage by p150CAF-1 promotes homologous recombination repair
title_full_unstemmed HP1α recruitment to DNA damage by p150CAF-1 promotes homologous recombination repair
title_short HP1α recruitment to DNA damage by p150CAF-1 promotes homologous recombination repair
title_sort hp1α recruitment to dna damage by p150caf-1 promotes homologous recombination repair
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3082177/
https://www.ncbi.nlm.nih.gov/pubmed/21464229
http://dx.doi.org/10.1083/jcb.201101030
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