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A presynaptic endosomal trafficking pathway controls synaptic growth signaling

Structural remodeling of synapses in response to growth signals leads to long-lasting alterations in neuronal function in many systems. Synaptic growth factor receptors alter their signaling properties during transit through the endocytic pathway, but the mechanisms controlling cargo traffic between...

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Autores principales: Rodal, Avital A., Blunk, Aline D., Akbergenova, Yulia, Jorquera, Ramon A., Buhl, Lauren K., Littleton, J. Troy
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3082179/
https://www.ncbi.nlm.nih.gov/pubmed/21464232
http://dx.doi.org/10.1083/jcb.201009052
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author Rodal, Avital A.
Blunk, Aline D.
Akbergenova, Yulia
Jorquera, Ramon A.
Buhl, Lauren K.
Littleton, J. Troy
author_facet Rodal, Avital A.
Blunk, Aline D.
Akbergenova, Yulia
Jorquera, Ramon A.
Buhl, Lauren K.
Littleton, J. Troy
author_sort Rodal, Avital A.
collection PubMed
description Structural remodeling of synapses in response to growth signals leads to long-lasting alterations in neuronal function in many systems. Synaptic growth factor receptors alter their signaling properties during transit through the endocytic pathway, but the mechanisms controlling cargo traffic between endocytic compartments remain unclear. Nwk (Nervous Wreck) is a presynaptic F-BAR/SH3 protein that regulates synaptic growth signaling in Drosophila melanogaster. In this paper, we show that Nwk acts through a physical interaction with sorting nexin 16 (SNX16). SNX16 promotes synaptic growth signaling by activated bone morphogenic protein receptors, and live imaging in neurons reveals that SNX16-positive early endosomes undergo transient interactions with Nwk-containing recycling endosomes. We identify an alternative signal termination pathway in the absence of Snx16 that is controlled by endosomal sorting complex required for transport (ESCRT)–mediated internalization of receptors into the endosomal lumen. Our results define a presynaptic trafficking pathway mediated by SNX16, NWK, and the ESCRT complex that functions to control synaptic growth signaling at the interface between endosomal compartments.
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spelling pubmed-30821792011-10-04 A presynaptic endosomal trafficking pathway controls synaptic growth signaling Rodal, Avital A. Blunk, Aline D. Akbergenova, Yulia Jorquera, Ramon A. Buhl, Lauren K. Littleton, J. Troy J Cell Biol Research Articles Structural remodeling of synapses in response to growth signals leads to long-lasting alterations in neuronal function in many systems. Synaptic growth factor receptors alter their signaling properties during transit through the endocytic pathway, but the mechanisms controlling cargo traffic between endocytic compartments remain unclear. Nwk (Nervous Wreck) is a presynaptic F-BAR/SH3 protein that regulates synaptic growth signaling in Drosophila melanogaster. In this paper, we show that Nwk acts through a physical interaction with sorting nexin 16 (SNX16). SNX16 promotes synaptic growth signaling by activated bone morphogenic protein receptors, and live imaging in neurons reveals that SNX16-positive early endosomes undergo transient interactions with Nwk-containing recycling endosomes. We identify an alternative signal termination pathway in the absence of Snx16 that is controlled by endosomal sorting complex required for transport (ESCRT)–mediated internalization of receptors into the endosomal lumen. Our results define a presynaptic trafficking pathway mediated by SNX16, NWK, and the ESCRT complex that functions to control synaptic growth signaling at the interface between endosomal compartments. The Rockefeller University Press 2011-04-04 /pmc/articles/PMC3082179/ /pubmed/21464232 http://dx.doi.org/10.1083/jcb.201009052 Text en © 2011 Rodal et al. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).
spellingShingle Research Articles
Rodal, Avital A.
Blunk, Aline D.
Akbergenova, Yulia
Jorquera, Ramon A.
Buhl, Lauren K.
Littleton, J. Troy
A presynaptic endosomal trafficking pathway controls synaptic growth signaling
title A presynaptic endosomal trafficking pathway controls synaptic growth signaling
title_full A presynaptic endosomal trafficking pathway controls synaptic growth signaling
title_fullStr A presynaptic endosomal trafficking pathway controls synaptic growth signaling
title_full_unstemmed A presynaptic endosomal trafficking pathway controls synaptic growth signaling
title_short A presynaptic endosomal trafficking pathway controls synaptic growth signaling
title_sort presynaptic endosomal trafficking pathway controls synaptic growth signaling
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3082179/
https://www.ncbi.nlm.nih.gov/pubmed/21464232
http://dx.doi.org/10.1083/jcb.201009052
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