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Expression of MICA, MICB and NKG2D in human leukemic myelomonocytic and cervical cancer cells

BACKGROUND: Cancer cells are known to secrete the stress molecules MICA and MICB that activate cytotoxicity by lymphocytes and NK cells through their NKG2D receptor as a mechanism of immunological defense. This work was undertaken to evaluate if cancer cells can also express this receptor as a possi...

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Autores principales: Weiss-Steider, Benny, Soto-Cruz, Isabel, Martinez-Campos, Christian A, Mendoza-Rincon, Jorge Flavio
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3082231/
https://www.ncbi.nlm.nih.gov/pubmed/21477352
http://dx.doi.org/10.1186/1756-9966-30-37
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author Weiss-Steider, Benny
Soto-Cruz, Isabel
Martinez-Campos, Christian A
Mendoza-Rincon, Jorge Flavio
author_facet Weiss-Steider, Benny
Soto-Cruz, Isabel
Martinez-Campos, Christian A
Mendoza-Rincon, Jorge Flavio
author_sort Weiss-Steider, Benny
collection PubMed
description BACKGROUND: Cancer cells are known to secrete the stress molecules MICA and MICB that activate cytotoxicity by lymphocytes and NK cells through their NKG2D receptor as a mechanism of immunological defense. This work was undertaken to evaluate if cancer cells can also express this receptor as a possible mechanisms of depletion of MIC molecules and thus interfere with their immune recognition. METHODS: Myelomonocytic leukemic (TPH-1 and U-937) and cervical cancer (CALO and INBL) cell lines were evaluated by Western Blot, ELISA, flow cytometry and immunocytochemistry to evaluate their capacity to express and secrete MICA and MICB and to be induced to proliferate by these molecules as well as to express their receptor NKG2D. Statistical analysis was performed by two-way ANOVA for time course analysis and Student's t-test for comparison between groups. Values were considered significantly different if p < 0.05. RESULTS: THP-1 and U-937 produce and secrete the stress MICA and MICB as shown by Western Blot of lysed cells and by ELISA of their conditioned media. By Western Blot and flow cytometry we found that these cells also express the receptor NKG2D. When THP-1 and U-937 were cultured with recombinant MICA and MICB they exhibited a dose dependent induction for their proliferation. CALO and INBL also produce MICA and MICB and were induced to proliferate by these stress molecules. By Western Blot, flow cytometry and immunocytochemistry we also found that these cells express NKG2D. CONCLUSIONS: Our novel results that tumor cells can simultaneously secrete MIC molecules and express their receptor, and to be induced for proliferation by these stress molecules, and that tumor epithelial cells can also express the NKG2D receptor that was thought to be exclusive of NK and cytotoxic lymphocytes is discussed as a possible mechanism of immunological escape and of tumor growth induction.
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spelling pubmed-30822312011-04-27 Expression of MICA, MICB and NKG2D in human leukemic myelomonocytic and cervical cancer cells Weiss-Steider, Benny Soto-Cruz, Isabel Martinez-Campos, Christian A Mendoza-Rincon, Jorge Flavio J Exp Clin Cancer Res Research BACKGROUND: Cancer cells are known to secrete the stress molecules MICA and MICB that activate cytotoxicity by lymphocytes and NK cells through their NKG2D receptor as a mechanism of immunological defense. This work was undertaken to evaluate if cancer cells can also express this receptor as a possible mechanisms of depletion of MIC molecules and thus interfere with their immune recognition. METHODS: Myelomonocytic leukemic (TPH-1 and U-937) and cervical cancer (CALO and INBL) cell lines were evaluated by Western Blot, ELISA, flow cytometry and immunocytochemistry to evaluate their capacity to express and secrete MICA and MICB and to be induced to proliferate by these molecules as well as to express their receptor NKG2D. Statistical analysis was performed by two-way ANOVA for time course analysis and Student's t-test for comparison between groups. Values were considered significantly different if p < 0.05. RESULTS: THP-1 and U-937 produce and secrete the stress MICA and MICB as shown by Western Blot of lysed cells and by ELISA of their conditioned media. By Western Blot and flow cytometry we found that these cells also express the receptor NKG2D. When THP-1 and U-937 were cultured with recombinant MICA and MICB they exhibited a dose dependent induction for their proliferation. CALO and INBL also produce MICA and MICB and were induced to proliferate by these stress molecules. By Western Blot, flow cytometry and immunocytochemistry we also found that these cells express NKG2D. CONCLUSIONS: Our novel results that tumor cells can simultaneously secrete MIC molecules and express their receptor, and to be induced for proliferation by these stress molecules, and that tumor epithelial cells can also express the NKG2D receptor that was thought to be exclusive of NK and cytotoxic lymphocytes is discussed as a possible mechanism of immunological escape and of tumor growth induction. BioMed Central 2011-04-10 /pmc/articles/PMC3082231/ /pubmed/21477352 http://dx.doi.org/10.1186/1756-9966-30-37 Text en Copyright ©2011 Weiss-Steider et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
Weiss-Steider, Benny
Soto-Cruz, Isabel
Martinez-Campos, Christian A
Mendoza-Rincon, Jorge Flavio
Expression of MICA, MICB and NKG2D in human leukemic myelomonocytic and cervical cancer cells
title Expression of MICA, MICB and NKG2D in human leukemic myelomonocytic and cervical cancer cells
title_full Expression of MICA, MICB and NKG2D in human leukemic myelomonocytic and cervical cancer cells
title_fullStr Expression of MICA, MICB and NKG2D in human leukemic myelomonocytic and cervical cancer cells
title_full_unstemmed Expression of MICA, MICB and NKG2D in human leukemic myelomonocytic and cervical cancer cells
title_short Expression of MICA, MICB and NKG2D in human leukemic myelomonocytic and cervical cancer cells
title_sort expression of mica, micb and nkg2d in human leukemic myelomonocytic and cervical cancer cells
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3082231/
https://www.ncbi.nlm.nih.gov/pubmed/21477352
http://dx.doi.org/10.1186/1756-9966-30-37
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