Measuring thermodynamic details of DNA hybridization using fluorescence

Modern real-time PCR systems make it easy to monitor fluorescence while temperature is varied for hundreds of samples in parallel, permitting high-throughput studies. We employed such system to investigate melting transitions of ordered nucleic acid structures into disordered random coils. Fluoresce...

Descripción completa

Detalles Bibliográficos
Autores principales: You, Yong, Tataurov, Andrey V, Owczarzy, Richard
Formato: Texto
Lenguaje:English
Publicado: Wiley Subscription Services, Inc., A Wiley Company 2011
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3082624/
https://www.ncbi.nlm.nih.gov/pubmed/21384337
http://dx.doi.org/10.1002/bip.21615

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3082624/
https://www.ncbi.nlm.nih.gov/pubmed/21384337
http://dx.doi.org/10.1002/bip.21615

Ejemplares similares