VCre/VloxP and SCre/SloxP: new site-specific recombination systems for genome engineering

We developed two new site-specific recombination systems named VCre/VloxP and SCre/SloxP for genome engineering. Their recognition sites are different from Cre recognition sites because VCre and SCre recombinases share less protein similarity with Cre, even though the basic 13-8-13 structures of the...

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Detalles Bibliográficos
Autores principales: Suzuki, Emiko, Nakayama, Manabu
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2011
Materias:
Methods Online
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3082901/
https://www.ncbi.nlm.nih.gov/pubmed/21288882
http://dx.doi.org/10.1093/nar/gkq1280
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author Suzuki, Emiko
Nakayama, Manabu
author_facet Suzuki, Emiko
Nakayama, Manabu
author_sort Suzuki, Emiko
collection PubMed
description We developed two new site-specific recombination systems named VCre/VloxP and SCre/SloxP for genome engineering. Their recognition sites are different from Cre recognition sites because VCre and SCre recombinases share less protein similarity with Cre, even though the basic 13-8-13 structures of their recognition sites are identical. Mutant VloxP and SloxP, which have the same uses as mutant loxP, were also developed. VCre/VloxP and SCre/SloxP in combination with Cre/loxP and Flp/FRT systems can serve as powerful tools for genome engineering, especially when used to genetically modify both alleles of a single gene in mouse and human cells.
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spelling pubmed-30829012011-04-27 VCre/VloxP and SCre/SloxP: new site-specific recombination systems for genome engineering Suzuki, Emiko Nakayama, Manabu Nucleic Acids Res Methods Online We developed two new site-specific recombination systems named VCre/VloxP and SCre/SloxP for genome engineering. Their recognition sites are different from Cre recognition sites because VCre and SCre recombinases share less protein similarity with Cre, even though the basic 13-8-13 structures of their recognition sites are identical. Mutant VloxP and SloxP, which have the same uses as mutant loxP, were also developed. VCre/VloxP and SCre/SloxP in combination with Cre/loxP and Flp/FRT systems can serve as powerful tools for genome engineering, especially when used to genetically modify both alleles of a single gene in mouse and human cells. Oxford University Press 2011-04 2011-02-01 /pmc/articles/PMC3082901/ /pubmed/21288882 http://dx.doi.org/10.1093/nar/gkq1280 Text en © The Author(s) 2011. Published by Oxford University Press. http://creativecommons.org/licenses/by-nc/2.5 This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Methods Online
Suzuki, Emiko
Nakayama, Manabu
VCre/VloxP and SCre/SloxP: new site-specific recombination systems for genome engineering
title VCre/VloxP and SCre/SloxP: new site-specific recombination systems for genome engineering
title_full VCre/VloxP and SCre/SloxP: new site-specific recombination systems for genome engineering
title_fullStr VCre/VloxP and SCre/SloxP: new site-specific recombination systems for genome engineering
title_full_unstemmed VCre/VloxP and SCre/SloxP: new site-specific recombination systems for genome engineering
title_short VCre/VloxP and SCre/SloxP: new site-specific recombination systems for genome engineering
title_sort vcre/vloxp and scre/sloxp: new site-specific recombination systems for genome engineering
topic Methods Online
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3082901/
https://www.ncbi.nlm.nih.gov/pubmed/21288882
http://dx.doi.org/10.1093/nar/gkq1280
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AT nakayamamanabu vcrevloxpandscresloxpnewsitespecificrecombinationsystemsforgenomeengineering

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