Molecular breeding of polymerases for resistance to environmental inhibitors

Potent inhibitors limit the use of PCR assays in a wide spectrum of specimens. Here, we describe the engineering of polymerases with a broad resistance to complex environmental inhibitors using molecular breeding of eight different polymerase orthologues from the genus Thermus and directed evolution...

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Detalles Bibliográficos
Autores principales: Baar, Claudia, d’Abbadie, Marc, Vaisman, Alexandra, Arana, Mercedes E., Hofreiter, Michael, Woodgate, Roger, Kunkel, Thomas A., Holliger, Philipp
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2011
Materias:
Methods Online
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3082909/
https://www.ncbi.nlm.nih.gov/pubmed/21297114
http://dx.doi.org/10.1093/nar/gkq1360

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3082909/
https://www.ncbi.nlm.nih.gov/pubmed/21297114
http://dx.doi.org/10.1093/nar/gkq1360

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