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In Vitro Binding Capacity of Bile Acids by Defatted Corn Protein Hydrolysate
Defatted corn protein was digested using five different proteases, Alcalase, Trypsin, Neutrase, Protamex and Flavourzyme, in order to produce bile acid binding peptides. Bile acid binding capacity was analyzed in vitro using peptides from different proteases of defatted corn hydrolysate. Some crysta...
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Formato: | Texto |
Lenguaje: | English |
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Molecular Diversity Preservation International (MDPI)
2011
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3083690/ https://www.ncbi.nlm.nih.gov/pubmed/21541043 |
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author | Kongo-Dia-Moukala, Jauricque Ursulla Zhang, Hui Irakoze, Pierre Claver |
author_facet | Kongo-Dia-Moukala, Jauricque Ursulla Zhang, Hui Irakoze, Pierre Claver |
author_sort | Kongo-Dia-Moukala, Jauricque Ursulla |
collection | PubMed |
description | Defatted corn protein was digested using five different proteases, Alcalase, Trypsin, Neutrase, Protamex and Flavourzyme, in order to produce bile acid binding peptides. Bile acid binding capacity was analyzed in vitro using peptides from different proteases of defatted corn hydrolysate. Some crystalline bile acids like sodium glycocholate, sodium cholate and sodium deoxycholate were individually tested using HPLC to see which enzymes can release more peptides with high bile acid binding capacity. Peptides from Flavourzyme defatted corn hydrolysate exhibited significantly (p < 0.05) stronger bile acid binding capacity than all others hydrolysates tested and all crystalline bile acids tested were highly bound by cholestyramine, a positive control well known as a cholesterol-reducing agent. The bile acid binding capacity of Flavourzyme hydrolysate was almost preserved after gastrointestinal proteases digestion. The molecular weight of Flavourzyme hydrolysate was determined and most of the peptides were found between 500–180 Da. The results showed that Flavourzyme hydrolysate may be used as a potential cholesterol-reducing agent. |
format | Text |
id | pubmed-3083690 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | Molecular Diversity Preservation International (MDPI) |
record_format | MEDLINE/PubMed |
spelling | pubmed-30836902011-05-03 In Vitro Binding Capacity of Bile Acids by Defatted Corn Protein Hydrolysate Kongo-Dia-Moukala, Jauricque Ursulla Zhang, Hui Irakoze, Pierre Claver Int J Mol Sci Article Defatted corn protein was digested using five different proteases, Alcalase, Trypsin, Neutrase, Protamex and Flavourzyme, in order to produce bile acid binding peptides. Bile acid binding capacity was analyzed in vitro using peptides from different proteases of defatted corn hydrolysate. Some crystalline bile acids like sodium glycocholate, sodium cholate and sodium deoxycholate were individually tested using HPLC to see which enzymes can release more peptides with high bile acid binding capacity. Peptides from Flavourzyme defatted corn hydrolysate exhibited significantly (p < 0.05) stronger bile acid binding capacity than all others hydrolysates tested and all crystalline bile acids tested were highly bound by cholestyramine, a positive control well known as a cholesterol-reducing agent. The bile acid binding capacity of Flavourzyme hydrolysate was almost preserved after gastrointestinal proteases digestion. The molecular weight of Flavourzyme hydrolysate was determined and most of the peptides were found between 500–180 Da. The results showed that Flavourzyme hydrolysate may be used as a potential cholesterol-reducing agent. Molecular Diversity Preservation International (MDPI) 2011-02-08 /pmc/articles/PMC3083690/ /pubmed/21541043 Text en © 2011 by the authors; licensee MDPI, Basel, Switzerland. http://creativecommons.org/licenses/by/3.0 This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/). |
spellingShingle | Article Kongo-Dia-Moukala, Jauricque Ursulla Zhang, Hui Irakoze, Pierre Claver In Vitro Binding Capacity of Bile Acids by Defatted Corn Protein Hydrolysate |
title | In Vitro Binding Capacity of Bile Acids by Defatted Corn Protein Hydrolysate |
title_full | In Vitro Binding Capacity of Bile Acids by Defatted Corn Protein Hydrolysate |
title_fullStr | In Vitro Binding Capacity of Bile Acids by Defatted Corn Protein Hydrolysate |
title_full_unstemmed | In Vitro Binding Capacity of Bile Acids by Defatted Corn Protein Hydrolysate |
title_short | In Vitro Binding Capacity of Bile Acids by Defatted Corn Protein Hydrolysate |
title_sort | in vitro binding capacity of bile acids by defatted corn protein hydrolysate |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3083690/ https://www.ncbi.nlm.nih.gov/pubmed/21541043 |
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