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N-Acetyl Glucosamine Obtained from Chitin by Chitin Degrading Factors in Chitinbacter tainanesis
A novel chitin-degrading aerobe, Chitinibacter tainanensis, was isolated from a soil sample from southern Taiwan, and was proved to produce N-acetyl glucosamine (NAG). Chitin degrading factors (CDFs) were proposed to be the critical factors to degrade chitin in this work. When C. tainanensis was inc...
Autores principales: | , , , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
Molecular Diversity Preservation International (MDPI)
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3083699/ https://www.ncbi.nlm.nih.gov/pubmed/21541052 http://dx.doi.org/10.3390/ijms12021187 |
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author | Chen, Jeen-Kuan Shen, Chia-Rui Yeh, Chao-Hsien Fang, Bing-Shiun Huang, Tung-Li Liu, Chao-Lin |
author_facet | Chen, Jeen-Kuan Shen, Chia-Rui Yeh, Chao-Hsien Fang, Bing-Shiun Huang, Tung-Li Liu, Chao-Lin |
author_sort | Chen, Jeen-Kuan |
collection | PubMed |
description | A novel chitin-degrading aerobe, Chitinibacter tainanensis, was isolated from a soil sample from southern Taiwan, and was proved to produce N-acetyl glucosamine (NAG). Chitin degrading factors (CDFs) were proposed to be the critical factors to degrade chitin in this work. When C. tainanensis was incubated with chitin, CDFs were induced and chitin was converted to NAG. CDFs were found to be located on the surface of C. tainanensis. N-Acetylglucosaminidase (NAGase) and endochitinase activities were found in the debris, and the activity of NAGase was much higher than that of endochitinase. The optimum pH of the enzymatic activity was about 7.0, while that of NAG production by the debris was 5.3. These results suggested that some factors in the debris, in addition to NAGase and endochitinase, were crucial for chitin degradation. |
format | Text |
id | pubmed-3083699 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | Molecular Diversity Preservation International (MDPI) |
record_format | MEDLINE/PubMed |
spelling | pubmed-30836992011-05-03 N-Acetyl Glucosamine Obtained from Chitin by Chitin Degrading Factors in Chitinbacter tainanesis Chen, Jeen-Kuan Shen, Chia-Rui Yeh, Chao-Hsien Fang, Bing-Shiun Huang, Tung-Li Liu, Chao-Lin Int J Mol Sci Article A novel chitin-degrading aerobe, Chitinibacter tainanensis, was isolated from a soil sample from southern Taiwan, and was proved to produce N-acetyl glucosamine (NAG). Chitin degrading factors (CDFs) were proposed to be the critical factors to degrade chitin in this work. When C. tainanensis was incubated with chitin, CDFs were induced and chitin was converted to NAG. CDFs were found to be located on the surface of C. tainanensis. N-Acetylglucosaminidase (NAGase) and endochitinase activities were found in the debris, and the activity of NAGase was much higher than that of endochitinase. The optimum pH of the enzymatic activity was about 7.0, while that of NAG production by the debris was 5.3. These results suggested that some factors in the debris, in addition to NAGase and endochitinase, were crucial for chitin degradation. Molecular Diversity Preservation International (MDPI) 2011-02-17 /pmc/articles/PMC3083699/ /pubmed/21541052 http://dx.doi.org/10.3390/ijms12021187 Text en © 2011 by the authors; licensee MDPI, Basel, Switzerland. http://creativecommons.org/licenses/by/3.0 This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/). |
spellingShingle | Article Chen, Jeen-Kuan Shen, Chia-Rui Yeh, Chao-Hsien Fang, Bing-Shiun Huang, Tung-Li Liu, Chao-Lin N-Acetyl Glucosamine Obtained from Chitin by Chitin Degrading Factors in Chitinbacter tainanesis |
title | N-Acetyl Glucosamine Obtained from Chitin by Chitin Degrading Factors in Chitinbacter tainanesis |
title_full | N-Acetyl Glucosamine Obtained from Chitin by Chitin Degrading Factors in Chitinbacter tainanesis |
title_fullStr | N-Acetyl Glucosamine Obtained from Chitin by Chitin Degrading Factors in Chitinbacter tainanesis |
title_full_unstemmed | N-Acetyl Glucosamine Obtained from Chitin by Chitin Degrading Factors in Chitinbacter tainanesis |
title_short | N-Acetyl Glucosamine Obtained from Chitin by Chitin Degrading Factors in Chitinbacter tainanesis |
title_sort | n-acetyl glucosamine obtained from chitin by chitin degrading factors in chitinbacter tainanesis |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3083699/ https://www.ncbi.nlm.nih.gov/pubmed/21541052 http://dx.doi.org/10.3390/ijms12021187 |
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