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The Effects of G-CSF on Proliferation of Mouse Myocardial Microvascular Endothelial Cells
This paper explores the effect of granulocyte colony-stimulating factor (G-CSF) on mouse myocardial microvascular endothelial cell (CMECs) proliferation. CMECs were harvested from C57/BL6 mice. CMECs were cultured in medium containing G-CSF (0 ng/mL, 20 ng/mL, 40 ng/mL, 60 ng/mL) for five days. Prol...
Autores principales: | , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
Molecular Diversity Preservation International (MDPI)
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3083707/ https://www.ncbi.nlm.nih.gov/pubmed/21541060 http://dx.doi.org/10.3390/ijms12021306 |
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author | Li, Jiming Zou, Yunzeng Ge, Junbo Zhang, Daifu Guan, Aili Wu, Jian Li, Lei |
author_facet | Li, Jiming Zou, Yunzeng Ge, Junbo Zhang, Daifu Guan, Aili Wu, Jian Li, Lei |
author_sort | Li, Jiming |
collection | PubMed |
description | This paper explores the effect of granulocyte colony-stimulating factor (G-CSF) on mouse myocardial microvascular endothelial cell (CMECs) proliferation. CMECs were harvested from C57/BL6 mice. CMECs were cultured in medium containing G-CSF (0 ng/mL, 20 ng/mL, 40 ng/mL, 60 ng/mL) for five days. Proliferative activity of CMECs was examined by CCK-8 method. Hypoxia inducible factor-1 (HIF-1) and p53 expression levels was determined from the mRNA obtained by reverse transcription polymerase chain reaction (RT-PCR). Results showed that the purity quotient of the CMECs, which were cultured by the method of modified myocardial tissue explant culture, was higher than 95%. Compared with control untreated cells, the proliferative activity of CMECs and the expression level of HIF-1 mRNA in these cells were enhanced by G-CSF treatment, whereas the expression level of p53 mRNA was markedly reduced. It may be concluded that G-CSF could promote the proliferative activity of CMECs, which might be mediated by upregulation of HIF-1 and downregulation of p53. |
format | Text |
id | pubmed-3083707 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | Molecular Diversity Preservation International (MDPI) |
record_format | MEDLINE/PubMed |
spelling | pubmed-30837072011-05-03 The Effects of G-CSF on Proliferation of Mouse Myocardial Microvascular Endothelial Cells Li, Jiming Zou, Yunzeng Ge, Junbo Zhang, Daifu Guan, Aili Wu, Jian Li, Lei Int J Mol Sci Article This paper explores the effect of granulocyte colony-stimulating factor (G-CSF) on mouse myocardial microvascular endothelial cell (CMECs) proliferation. CMECs were harvested from C57/BL6 mice. CMECs were cultured in medium containing G-CSF (0 ng/mL, 20 ng/mL, 40 ng/mL, 60 ng/mL) for five days. Proliferative activity of CMECs was examined by CCK-8 method. Hypoxia inducible factor-1 (HIF-1) and p53 expression levels was determined from the mRNA obtained by reverse transcription polymerase chain reaction (RT-PCR). Results showed that the purity quotient of the CMECs, which were cultured by the method of modified myocardial tissue explant culture, was higher than 95%. Compared with control untreated cells, the proliferative activity of CMECs and the expression level of HIF-1 mRNA in these cells were enhanced by G-CSF treatment, whereas the expression level of p53 mRNA was markedly reduced. It may be concluded that G-CSF could promote the proliferative activity of CMECs, which might be mediated by upregulation of HIF-1 and downregulation of p53. Molecular Diversity Preservation International (MDPI) 2011-02-22 /pmc/articles/PMC3083707/ /pubmed/21541060 http://dx.doi.org/10.3390/ijms12021306 Text en © 2011 by the authors; licensee MDPI, Basel, Switzerland. http://creativecommons.org/licenses/by/3.0 This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/). |
spellingShingle | Article Li, Jiming Zou, Yunzeng Ge, Junbo Zhang, Daifu Guan, Aili Wu, Jian Li, Lei The Effects of G-CSF on Proliferation of Mouse Myocardial Microvascular Endothelial Cells |
title | The Effects of G-CSF on Proliferation of Mouse Myocardial Microvascular Endothelial Cells |
title_full | The Effects of G-CSF on Proliferation of Mouse Myocardial Microvascular Endothelial Cells |
title_fullStr | The Effects of G-CSF on Proliferation of Mouse Myocardial Microvascular Endothelial Cells |
title_full_unstemmed | The Effects of G-CSF on Proliferation of Mouse Myocardial Microvascular Endothelial Cells |
title_short | The Effects of G-CSF on Proliferation of Mouse Myocardial Microvascular Endothelial Cells |
title_sort | effects of g-csf on proliferation of mouse myocardial microvascular endothelial cells |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3083707/ https://www.ncbi.nlm.nih.gov/pubmed/21541060 http://dx.doi.org/10.3390/ijms12021306 |
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