Cargando…
Claudin 4 Is Differentially Expressed between Ovarian Cancer Subtypes and Plays a Role in Spheroid Formation
Claudin 4 is a cellular adhesion molecule that is frequently overexpressed in ovarian cancer and other epithelial cancers. In this study, we sought to determine whether the expression of claudin 4 is associated with outcome in ovarian cancer patients and may be involved in tumor progression. We exam...
Autores principales: | , , , , , , , , |
---|---|
Formato: | Texto |
Lenguaje: | English |
Publicado: |
Molecular Diversity Preservation International (MDPI)
2011
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3083709/ https://www.ncbi.nlm.nih.gov/pubmed/21541062 http://dx.doi.org/10.3390/ijms12021334 |
_version_ | 1782202445164707840 |
---|---|
author | Boylan, Kristin L. M. Misemer, Benjamin DeRycke, Melissa S. Andersen, John D. Harrington, Katherine M. Kalloger, Steve E. Gilks, C. Blake Pambuccian, Stefan E. Skubitz, Amy P. N. |
author_facet | Boylan, Kristin L. M. Misemer, Benjamin DeRycke, Melissa S. Andersen, John D. Harrington, Katherine M. Kalloger, Steve E. Gilks, C. Blake Pambuccian, Stefan E. Skubitz, Amy P. N. |
author_sort | Boylan, Kristin L. M. |
collection | PubMed |
description | Claudin 4 is a cellular adhesion molecule that is frequently overexpressed in ovarian cancer and other epithelial cancers. In this study, we sought to determine whether the expression of claudin 4 is associated with outcome in ovarian cancer patients and may be involved in tumor progression. We examined claudin 4 expression in ovarian cancer tissues and cell lines, as well as by immunohistochemical staining of tissue microarrays (TMAs; n = 500), spheroids present in patients’ ascites, and spheroids formed in vitro. Claudin 4 was expressed in nearly 70% of the ovarian cancer tissues examined and was differentially expressed across ovarian cancer subtypes, with the lowest expression in clear cell subtype. No association was found between claudin 4 expression and disease-specific survival in any subtype. Claudin 4 expression was also observed in multicellular spheroids obtained from patients’ ascites. Using an in vitro spheroid formation assay, we found that NIH:OVCAR5 cells treated with shRNA against claudin 4 required a longer time to form compact spheroids compared to control NIH:OVCAR5 cells that expressed high levels of claudin 4. The inability of the NIH:OVCAR5 cells treated with claudin 4 shRNA to form compact spheroids was verified by FITC-dextran exclusion. These results demonstrate a role for claudin 4 and tight junctions in spheroid formation and integrity. |
format | Text |
id | pubmed-3083709 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | Molecular Diversity Preservation International (MDPI) |
record_format | MEDLINE/PubMed |
spelling | pubmed-30837092011-05-03 Claudin 4 Is Differentially Expressed between Ovarian Cancer Subtypes and Plays a Role in Spheroid Formation Boylan, Kristin L. M. Misemer, Benjamin DeRycke, Melissa S. Andersen, John D. Harrington, Katherine M. Kalloger, Steve E. Gilks, C. Blake Pambuccian, Stefan E. Skubitz, Amy P. N. Int J Mol Sci Article Claudin 4 is a cellular adhesion molecule that is frequently overexpressed in ovarian cancer and other epithelial cancers. In this study, we sought to determine whether the expression of claudin 4 is associated with outcome in ovarian cancer patients and may be involved in tumor progression. We examined claudin 4 expression in ovarian cancer tissues and cell lines, as well as by immunohistochemical staining of tissue microarrays (TMAs; n = 500), spheroids present in patients’ ascites, and spheroids formed in vitro. Claudin 4 was expressed in nearly 70% of the ovarian cancer tissues examined and was differentially expressed across ovarian cancer subtypes, with the lowest expression in clear cell subtype. No association was found between claudin 4 expression and disease-specific survival in any subtype. Claudin 4 expression was also observed in multicellular spheroids obtained from patients’ ascites. Using an in vitro spheroid formation assay, we found that NIH:OVCAR5 cells treated with shRNA against claudin 4 required a longer time to form compact spheroids compared to control NIH:OVCAR5 cells that expressed high levels of claudin 4. The inability of the NIH:OVCAR5 cells treated with claudin 4 shRNA to form compact spheroids was verified by FITC-dextran exclusion. These results demonstrate a role for claudin 4 and tight junctions in spheroid formation and integrity. Molecular Diversity Preservation International (MDPI) 2011-02-22 /pmc/articles/PMC3083709/ /pubmed/21541062 http://dx.doi.org/10.3390/ijms12021334 Text en © 2011 by the authors; licensee MDPI, Basel, Switzerland. http://creativecommons.org/licenses/by/3.0 This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/). |
spellingShingle | Article Boylan, Kristin L. M. Misemer, Benjamin DeRycke, Melissa S. Andersen, John D. Harrington, Katherine M. Kalloger, Steve E. Gilks, C. Blake Pambuccian, Stefan E. Skubitz, Amy P. N. Claudin 4 Is Differentially Expressed between Ovarian Cancer Subtypes and Plays a Role in Spheroid Formation |
title | Claudin 4 Is Differentially Expressed between Ovarian Cancer Subtypes and Plays a Role in Spheroid Formation |
title_full | Claudin 4 Is Differentially Expressed between Ovarian Cancer Subtypes and Plays a Role in Spheroid Formation |
title_fullStr | Claudin 4 Is Differentially Expressed between Ovarian Cancer Subtypes and Plays a Role in Spheroid Formation |
title_full_unstemmed | Claudin 4 Is Differentially Expressed between Ovarian Cancer Subtypes and Plays a Role in Spheroid Formation |
title_short | Claudin 4 Is Differentially Expressed between Ovarian Cancer Subtypes and Plays a Role in Spheroid Formation |
title_sort | claudin 4 is differentially expressed between ovarian cancer subtypes and plays a role in spheroid formation |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3083709/ https://www.ncbi.nlm.nih.gov/pubmed/21541062 http://dx.doi.org/10.3390/ijms12021334 |
work_keys_str_mv | AT boylankristinlm claudin4isdifferentiallyexpressedbetweenovariancancersubtypesandplaysaroleinspheroidformation AT misemerbenjamin claudin4isdifferentiallyexpressedbetweenovariancancersubtypesandplaysaroleinspheroidformation AT deryckemelissas claudin4isdifferentiallyexpressedbetweenovariancancersubtypesandplaysaroleinspheroidformation AT andersenjohnd claudin4isdifferentiallyexpressedbetweenovariancancersubtypesandplaysaroleinspheroidformation AT harringtonkatherinem claudin4isdifferentiallyexpressedbetweenovariancancersubtypesandplaysaroleinspheroidformation AT kallogerstevee claudin4isdifferentiallyexpressedbetweenovariancancersubtypesandplaysaroleinspheroidformation AT gilkscblake claudin4isdifferentiallyexpressedbetweenovariancancersubtypesandplaysaroleinspheroidformation AT pambuccianstefane claudin4isdifferentiallyexpressedbetweenovariancancersubtypesandplaysaroleinspheroidformation AT skubitzamypn claudin4isdifferentiallyexpressedbetweenovariancancersubtypesandplaysaroleinspheroidformation |