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The Complete Genome Sequence of ‘Candidatus Liberibacter solanacearum’, the Bacterium Associated with Potato Zebra Chip Disease
Zebra Chip (ZC) is an emerging plant disease that causes aboveground decline of potato shoots and generally results in unusable tubers. This disease has led to multi-million dollar losses for growers in the central and western United States over the past decade and impacts the livelihood of potato f...
Autores principales: | , , , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
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Public Library of Science
2011
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3084294/ https://www.ncbi.nlm.nih.gov/pubmed/21552483 http://dx.doi.org/10.1371/journal.pone.0019135 |
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author | Lin, Hong Lou, Binghai Glynn, Jonathan M. Doddapaneni, Harshavardhan Civerolo, Edwin L. Chen, Chuanwu Duan, Yongping Zhou, Lijuan Vahling, Cheryl M. |
author_facet | Lin, Hong Lou, Binghai Glynn, Jonathan M. Doddapaneni, Harshavardhan Civerolo, Edwin L. Chen, Chuanwu Duan, Yongping Zhou, Lijuan Vahling, Cheryl M. |
author_sort | Lin, Hong |
collection | PubMed |
description | Zebra Chip (ZC) is an emerging plant disease that causes aboveground decline of potato shoots and generally results in unusable tubers. This disease has led to multi-million dollar losses for growers in the central and western United States over the past decade and impacts the livelihood of potato farmers in Mexico and New Zealand. ZC is associated with ‘Candidatus Liberibacter solanacearum’, a fastidious alpha-proteobacterium that is transmitted by a phloem-feeding psyllid vector, Bactericera cockerelli Sulc. Research on this disease has been hampered by a lack of robust culture methods and paucity of genome sequence information for ‘Ca. L. solanacearum’. Here we present the sequence of the 1.26 Mbp metagenome of ‘Ca. L. solanacearum’, based on DNA isolated from potato psyllids. The coding inventory of the ‘Ca. L. solanacearum’ genome was analyzed and compared to related Rhizobiaceae to better understand ‘Ca. L. solanacearum’ physiology and identify potential targets to develop improved treatment strategies. This analysis revealed a number of unique transporters and pathways, all potentially contributing to ZC pathogenesis. Some of these factors may have been acquired through horizontal gene transfer. Taxonomically, ‘Ca. L. solanacearum’ is related to ‘Ca. L. asiaticus’, a suspected causative agent of citrus huanglongbing, yet many genome rearrangements and several gene gains/losses are evident when comparing these two Liberibacter. species. Relative to ‘Ca. L. asiaticus’, ‘Ca. L. solanacearum’ probably has reduced capacity for nucleic acid modification, increased amino acid and vitamin biosynthesis functionalities, and gained a high-affinity iron transport system characteristic of several pathogenic microbes. |
format | Text |
id | pubmed-3084294 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-30842942011-05-06 The Complete Genome Sequence of ‘Candidatus Liberibacter solanacearum’, the Bacterium Associated with Potato Zebra Chip Disease Lin, Hong Lou, Binghai Glynn, Jonathan M. Doddapaneni, Harshavardhan Civerolo, Edwin L. Chen, Chuanwu Duan, Yongping Zhou, Lijuan Vahling, Cheryl M. PLoS One Research Article Zebra Chip (ZC) is an emerging plant disease that causes aboveground decline of potato shoots and generally results in unusable tubers. This disease has led to multi-million dollar losses for growers in the central and western United States over the past decade and impacts the livelihood of potato farmers in Mexico and New Zealand. ZC is associated with ‘Candidatus Liberibacter solanacearum’, a fastidious alpha-proteobacterium that is transmitted by a phloem-feeding psyllid vector, Bactericera cockerelli Sulc. Research on this disease has been hampered by a lack of robust culture methods and paucity of genome sequence information for ‘Ca. L. solanacearum’. Here we present the sequence of the 1.26 Mbp metagenome of ‘Ca. L. solanacearum’, based on DNA isolated from potato psyllids. The coding inventory of the ‘Ca. L. solanacearum’ genome was analyzed and compared to related Rhizobiaceae to better understand ‘Ca. L. solanacearum’ physiology and identify potential targets to develop improved treatment strategies. This analysis revealed a number of unique transporters and pathways, all potentially contributing to ZC pathogenesis. Some of these factors may have been acquired through horizontal gene transfer. Taxonomically, ‘Ca. L. solanacearum’ is related to ‘Ca. L. asiaticus’, a suspected causative agent of citrus huanglongbing, yet many genome rearrangements and several gene gains/losses are evident when comparing these two Liberibacter. species. Relative to ‘Ca. L. asiaticus’, ‘Ca. L. solanacearum’ probably has reduced capacity for nucleic acid modification, increased amino acid and vitamin biosynthesis functionalities, and gained a high-affinity iron transport system characteristic of several pathogenic microbes. Public Library of Science 2011-04-28 /pmc/articles/PMC3084294/ /pubmed/21552483 http://dx.doi.org/10.1371/journal.pone.0019135 Text en This is an open-access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 public domain dedication. https://creativecommons.org/publicdomain/zero/1.0/ This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. |
spellingShingle | Research Article Lin, Hong Lou, Binghai Glynn, Jonathan M. Doddapaneni, Harshavardhan Civerolo, Edwin L. Chen, Chuanwu Duan, Yongping Zhou, Lijuan Vahling, Cheryl M. The Complete Genome Sequence of ‘Candidatus Liberibacter solanacearum’, the Bacterium Associated with Potato Zebra Chip Disease |
title | The Complete Genome Sequence of ‘Candidatus
Liberibacter solanacearum’, the Bacterium Associated with Potato Zebra
Chip Disease |
title_full | The Complete Genome Sequence of ‘Candidatus
Liberibacter solanacearum’, the Bacterium Associated with Potato Zebra
Chip Disease |
title_fullStr | The Complete Genome Sequence of ‘Candidatus
Liberibacter solanacearum’, the Bacterium Associated with Potato Zebra
Chip Disease |
title_full_unstemmed | The Complete Genome Sequence of ‘Candidatus
Liberibacter solanacearum’, the Bacterium Associated with Potato Zebra
Chip Disease |
title_short | The Complete Genome Sequence of ‘Candidatus
Liberibacter solanacearum’, the Bacterium Associated with Potato Zebra
Chip Disease |
title_sort | complete genome sequence of ‘candidatus
liberibacter solanacearum’, the bacterium associated with potato zebra
chip disease |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3084294/ https://www.ncbi.nlm.nih.gov/pubmed/21552483 http://dx.doi.org/10.1371/journal.pone.0019135 |
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