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Intraperoxisomal redox balance in mammalian cells: oxidative stress and interorganellar cross-talk

Reactive oxygen species (ROS) are at once unsought by-products of metabolism and critical regulators of multiple intracellular signaling cascades. In nonphotosynthetic eukaryotic cells, mitochondria are well-investigated major sites of ROS generation and related signal initiation. Peroxisomes are al...

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Autores principales: Ivashchenko, Oksana, Van Veldhoven, Paul P., Brees, Chantal, Ho, Ye-Shih, Terlecky, Stanley R., Fransen, Marc
Formato: Texto
Lenguaje:English
Publicado: The American Society for Cell Biology 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3084667/
https://www.ncbi.nlm.nih.gov/pubmed/21372177
http://dx.doi.org/10.1091/mbc.E10-11-0919
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author Ivashchenko, Oksana
Van Veldhoven, Paul P.
Brees, Chantal
Ho, Ye-Shih
Terlecky, Stanley R.
Fransen, Marc
author_facet Ivashchenko, Oksana
Van Veldhoven, Paul P.
Brees, Chantal
Ho, Ye-Shih
Terlecky, Stanley R.
Fransen, Marc
author_sort Ivashchenko, Oksana
collection PubMed
description Reactive oxygen species (ROS) are at once unsought by-products of metabolism and critical regulators of multiple intracellular signaling cascades. In nonphotosynthetic eukaryotic cells, mitochondria are well-investigated major sites of ROS generation and related signal initiation. Peroxisomes are also capable of ROS generation, but their contribution to cellular oxidation–reduction (redox) balance and signaling events are far less well understood. In this study, we use a redox-sensitive variant of enhanced green fluorescent protein (roGFP2-PTS1) to monitor the state of the peroxisomal matrix in mammalian cells. We show that intraperoxisomal redox status is strongly influenced by environmental growth conditions. Furthermore, disturbances in peroxisomal redox balance, although not necessarily correlated with the age of the organelle, may trigger its degradation. We also demonstrate that the mitochondrial redox balance is perturbed in catalase-deficient cells and upon generation of excess ROS inside peroxisomes. Peroxisomes are found to resist oxidative stress generated elsewhere in the cell but are affected when the burden originates within the organelle. These results suggest a potential broader role for the peroxisome in cellular aging and the initiation of age-related degenerative disease.
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spelling pubmed-30846672011-07-16 Intraperoxisomal redox balance in mammalian cells: oxidative stress and interorganellar cross-talk Ivashchenko, Oksana Van Veldhoven, Paul P. Brees, Chantal Ho, Ye-Shih Terlecky, Stanley R. Fransen, Marc Mol Biol Cell Articles Reactive oxygen species (ROS) are at once unsought by-products of metabolism and critical regulators of multiple intracellular signaling cascades. In nonphotosynthetic eukaryotic cells, mitochondria are well-investigated major sites of ROS generation and related signal initiation. Peroxisomes are also capable of ROS generation, but their contribution to cellular oxidation–reduction (redox) balance and signaling events are far less well understood. In this study, we use a redox-sensitive variant of enhanced green fluorescent protein (roGFP2-PTS1) to monitor the state of the peroxisomal matrix in mammalian cells. We show that intraperoxisomal redox status is strongly influenced by environmental growth conditions. Furthermore, disturbances in peroxisomal redox balance, although not necessarily correlated with the age of the organelle, may trigger its degradation. We also demonstrate that the mitochondrial redox balance is perturbed in catalase-deficient cells and upon generation of excess ROS inside peroxisomes. Peroxisomes are found to resist oxidative stress generated elsewhere in the cell but are affected when the burden originates within the organelle. These results suggest a potential broader role for the peroxisome in cellular aging and the initiation of age-related degenerative disease. The American Society for Cell Biology 2011-05-01 /pmc/articles/PMC3084667/ /pubmed/21372177 http://dx.doi.org/10.1091/mbc.E10-11-0919 Text en © 2011 Ivashchenko et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0). “ASCB®,“ “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society of Cell Biology.
spellingShingle Articles
Ivashchenko, Oksana
Van Veldhoven, Paul P.
Brees, Chantal
Ho, Ye-Shih
Terlecky, Stanley R.
Fransen, Marc
Intraperoxisomal redox balance in mammalian cells: oxidative stress and interorganellar cross-talk
title Intraperoxisomal redox balance in mammalian cells: oxidative stress and interorganellar cross-talk
title_full Intraperoxisomal redox balance in mammalian cells: oxidative stress and interorganellar cross-talk
title_fullStr Intraperoxisomal redox balance in mammalian cells: oxidative stress and interorganellar cross-talk
title_full_unstemmed Intraperoxisomal redox balance in mammalian cells: oxidative stress and interorganellar cross-talk
title_short Intraperoxisomal redox balance in mammalian cells: oxidative stress and interorganellar cross-talk
title_sort intraperoxisomal redox balance in mammalian cells: oxidative stress and interorganellar cross-talk
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3084667/
https://www.ncbi.nlm.nih.gov/pubmed/21372177
http://dx.doi.org/10.1091/mbc.E10-11-0919
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