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N-cadherin–mediated cell adhesion restricts cell proliferation in the dorsal neural tube
Neural progenitors are organized as a pseudostratified epithelium held together by adherens junctions (AJs), multiprotein complexes composed of cadherins and α- and β-catenin. Catenins are known to control neural progenitor division; however, it is not known whether they function in this capacity as...
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Formato: | Texto |
Lenguaje: | English |
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The American Society for Cell Biology
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3084673/ https://www.ncbi.nlm.nih.gov/pubmed/21389116 http://dx.doi.org/10.1091/mbc.E10-08-0675 |
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author | Chalasani, Kavita Brewster, Rachel M. |
author_facet | Chalasani, Kavita Brewster, Rachel M. |
author_sort | Chalasani, Kavita |
collection | PubMed |
description | Neural progenitors are organized as a pseudostratified epithelium held together by adherens junctions (AJs), multiprotein complexes composed of cadherins and α- and β-catenin. Catenins are known to control neural progenitor division; however, it is not known whether they function in this capacity as cadherin binding partners, as there is little evidence that cadherins themselves regulate neural proliferation. We show here that zebrafish N-cadherin (N-cad) restricts cell proliferation in the dorsal region of the neural tube by regulating cell-cycle length. We further reveal that N-cad couples cell-cycle exit and differentiation, as a fraction of neurons are mitotic in N-cad mutants. Enhanced proliferation in N-cad mutants is mediated by ligand-independent activation of Hedgehog (Hh) signaling, possibly caused by defective ciliogenesis. Furthermore, depletion of Hh signaling results in the loss of junctional markers. We therefore propose that N-cad restricts the response of dorsal neural progenitors to Hh and that Hh signaling limits the range of its own activity by promoting AJ assembly. Taken together, these observations emphasize a key role for N-cad–mediated adhesion in controlling neural progenitor proliferation. In addition, these findings are the first to demonstrate a requirement for cadherins in synchronizing cell-cycle exit and differentiation and a reciprocal interaction between AJs and Hh signaling. |
format | Text |
id | pubmed-3084673 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | The American Society for Cell Biology |
record_format | MEDLINE/PubMed |
spelling | pubmed-30846732011-07-16 N-cadherin–mediated cell adhesion restricts cell proliferation in the dorsal neural tube Chalasani, Kavita Brewster, Rachel M. Mol Biol Cell Articles Neural progenitors are organized as a pseudostratified epithelium held together by adherens junctions (AJs), multiprotein complexes composed of cadherins and α- and β-catenin. Catenins are known to control neural progenitor division; however, it is not known whether they function in this capacity as cadherin binding partners, as there is little evidence that cadherins themselves regulate neural proliferation. We show here that zebrafish N-cadherin (N-cad) restricts cell proliferation in the dorsal region of the neural tube by regulating cell-cycle length. We further reveal that N-cad couples cell-cycle exit and differentiation, as a fraction of neurons are mitotic in N-cad mutants. Enhanced proliferation in N-cad mutants is mediated by ligand-independent activation of Hedgehog (Hh) signaling, possibly caused by defective ciliogenesis. Furthermore, depletion of Hh signaling results in the loss of junctional markers. We therefore propose that N-cad restricts the response of dorsal neural progenitors to Hh and that Hh signaling limits the range of its own activity by promoting AJ assembly. Taken together, these observations emphasize a key role for N-cad–mediated adhesion in controlling neural progenitor proliferation. In addition, these findings are the first to demonstrate a requirement for cadherins in synchronizing cell-cycle exit and differentiation and a reciprocal interaction between AJs and Hh signaling. The American Society for Cell Biology 2011-05-01 /pmc/articles/PMC3084673/ /pubmed/21389116 http://dx.doi.org/10.1091/mbc.E10-08-0675 Text en © 2011 Chalasani and Brewster. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0). “ASCB®,“ “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society of Cell Biology. |
spellingShingle | Articles Chalasani, Kavita Brewster, Rachel M. N-cadherin–mediated cell adhesion restricts cell proliferation in the dorsal neural tube |
title | N-cadherin–mediated cell adhesion restricts cell proliferation in the dorsal neural tube |
title_full | N-cadherin–mediated cell adhesion restricts cell proliferation in the dorsal neural tube |
title_fullStr | N-cadherin–mediated cell adhesion restricts cell proliferation in the dorsal neural tube |
title_full_unstemmed | N-cadherin–mediated cell adhesion restricts cell proliferation in the dorsal neural tube |
title_short | N-cadherin–mediated cell adhesion restricts cell proliferation in the dorsal neural tube |
title_sort | n-cadherin–mediated cell adhesion restricts cell proliferation in the dorsal neural tube |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3084673/ https://www.ncbi.nlm.nih.gov/pubmed/21389116 http://dx.doi.org/10.1091/mbc.E10-08-0675 |
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