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Kinesin-5, a mitotic microtubule-associated motor protein, modulates neuronal migration
Kinesin-5 (also called Eg5 or kif11) is a homotetrameric motor protein that functions by modulating microtubule (MT)–MT interactions. In the case of mitosis, kinesin-5 slows the rate of separation of the half-spindles. In the case of the axon, kinesin-5 limits the frequency of transport of short MTs...
Autores principales: | , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
The American Society for Cell Biology
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3084678/ https://www.ncbi.nlm.nih.gov/pubmed/21411631 http://dx.doi.org/10.1091/mbc.E10-11-0905 |
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author | Falnikar, Aditi Tole, Shubha Baas, Peter W. |
author_facet | Falnikar, Aditi Tole, Shubha Baas, Peter W. |
author_sort | Falnikar, Aditi |
collection | PubMed |
description | Kinesin-5 (also called Eg5 or kif11) is a homotetrameric motor protein that functions by modulating microtubule (MT)–MT interactions. In the case of mitosis, kinesin-5 slows the rate of separation of the half-spindles. In the case of the axon, kinesin-5 limits the frequency of transport of short MTs, and also limits the rate of axonal growth. Here we show that experimental inhibition of kinesin-5 in cultured migratory neurons results in a faster but more randomly moving neuron with a shorter leading process. As is the case with axons of stationary neurons, short MT transport frequency is notably enhanced in the leading process of the migratory neuron when kinesin-5 is inhibited. Conversely, overexpression of kinesin-5, both in culture and in developing cerebral cortex, causes migration to slow and even cease. Regions of anti-parallel MT organization behind the centrosome were shown to be especially rich in kinesin-5, implicating these regions as potential sites where kinesin-5 forces may be especially relevant. We posit that kinesin-5 acts as a “brake” on MT–MT interactions that modulates the advance of the entire MT apparatus. In so doing, kinesin-5 regulates the rate and directionality of neuronal migration and possibly the cessation of migration when the neuron reaches its destination. |
format | Text |
id | pubmed-3084678 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | The American Society for Cell Biology |
record_format | MEDLINE/PubMed |
spelling | pubmed-30846782011-07-16 Kinesin-5, a mitotic microtubule-associated motor protein, modulates neuronal migration Falnikar, Aditi Tole, Shubha Baas, Peter W. Mol Biol Cell Articles Kinesin-5 (also called Eg5 or kif11) is a homotetrameric motor protein that functions by modulating microtubule (MT)–MT interactions. In the case of mitosis, kinesin-5 slows the rate of separation of the half-spindles. In the case of the axon, kinesin-5 limits the frequency of transport of short MTs, and also limits the rate of axonal growth. Here we show that experimental inhibition of kinesin-5 in cultured migratory neurons results in a faster but more randomly moving neuron with a shorter leading process. As is the case with axons of stationary neurons, short MT transport frequency is notably enhanced in the leading process of the migratory neuron when kinesin-5 is inhibited. Conversely, overexpression of kinesin-5, both in culture and in developing cerebral cortex, causes migration to slow and even cease. Regions of anti-parallel MT organization behind the centrosome were shown to be especially rich in kinesin-5, implicating these regions as potential sites where kinesin-5 forces may be especially relevant. We posit that kinesin-5 acts as a “brake” on MT–MT interactions that modulates the advance of the entire MT apparatus. In so doing, kinesin-5 regulates the rate and directionality of neuronal migration and possibly the cessation of migration when the neuron reaches its destination. The American Society for Cell Biology 2011-05-01 /pmc/articles/PMC3084678/ /pubmed/21411631 http://dx.doi.org/10.1091/mbc.E10-11-0905 Text en © 2011 Falnikar et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0). “ASCB®,“ “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society of Cell Biology. |
spellingShingle | Articles Falnikar, Aditi Tole, Shubha Baas, Peter W. Kinesin-5, a mitotic microtubule-associated motor protein, modulates neuronal migration |
title | Kinesin-5, a mitotic microtubule-associated motor protein, modulates neuronal migration |
title_full | Kinesin-5, a mitotic microtubule-associated motor protein, modulates neuronal migration |
title_fullStr | Kinesin-5, a mitotic microtubule-associated motor protein, modulates neuronal migration |
title_full_unstemmed | Kinesin-5, a mitotic microtubule-associated motor protein, modulates neuronal migration |
title_short | Kinesin-5, a mitotic microtubule-associated motor protein, modulates neuronal migration |
title_sort | kinesin-5, a mitotic microtubule-associated motor protein, modulates neuronal migration |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3084678/ https://www.ncbi.nlm.nih.gov/pubmed/21411631 http://dx.doi.org/10.1091/mbc.E10-11-0905 |
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