Cargando…

Morphological and Structural Aspects of the Extremely Halophilic Archaeon Haloquadratum walsbyi

Ultrathin square cell Haloquadratum walsbyi from the Archaea domain are the most abundant microorganisms in the hypersaline water of coastal salterns and continental salt lakes. In this work, we explore the cell surface of these microorganisms using amplitude-modulation atomic-force microscopy in ne...

Descripción completa

Detalles Bibliográficos
Autores principales: Sublimi Saponetti, Matilde, Bobba, Fabrizio, Salerno, Grazia, Scarfato, Alessandro, Corcelli, Angela, Cucolo, Annamaria
Formato: Texto
Lenguaje:English
Publicado: Public Library of Science 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3084702/
https://www.ncbi.nlm.nih.gov/pubmed/21559517
http://dx.doi.org/10.1371/journal.pone.0018653
_version_ 1782202534021038080
author Sublimi Saponetti, Matilde
Bobba, Fabrizio
Salerno, Grazia
Scarfato, Alessandro
Corcelli, Angela
Cucolo, Annamaria
author_facet Sublimi Saponetti, Matilde
Bobba, Fabrizio
Salerno, Grazia
Scarfato, Alessandro
Corcelli, Angela
Cucolo, Annamaria
author_sort Sublimi Saponetti, Matilde
collection PubMed
description Ultrathin square cell Haloquadratum walsbyi from the Archaea domain are the most abundant microorganisms in the hypersaline water of coastal salterns and continental salt lakes. In this work, we explore the cell surface of these microorganisms using amplitude-modulation atomic-force microscopy in nearly physiological conditions. We demonstrate the presence of a regular corrugation with a periodicity of 16–20 nm attributed to the surface layer (S-layer) protein lattice, striped domains asymmetrically distributed on the cell faces and peculiar bulges correlated with the presence of intracellular granules. Besides, subsequent images of cell evolution during the drying process indicate the presence of an external capsule that might correspond to the giant protein halomucin, predicted by the genome but never before observed by other microscopy studies.
format Text
id pubmed-3084702
institution National Center for Biotechnology Information
language English
publishDate 2011
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-30847022011-05-10 Morphological and Structural Aspects of the Extremely Halophilic Archaeon Haloquadratum walsbyi Sublimi Saponetti, Matilde Bobba, Fabrizio Salerno, Grazia Scarfato, Alessandro Corcelli, Angela Cucolo, Annamaria PLoS One Research Article Ultrathin square cell Haloquadratum walsbyi from the Archaea domain are the most abundant microorganisms in the hypersaline water of coastal salterns and continental salt lakes. In this work, we explore the cell surface of these microorganisms using amplitude-modulation atomic-force microscopy in nearly physiological conditions. We demonstrate the presence of a regular corrugation with a periodicity of 16–20 nm attributed to the surface layer (S-layer) protein lattice, striped domains asymmetrically distributed on the cell faces and peculiar bulges correlated with the presence of intracellular granules. Besides, subsequent images of cell evolution during the drying process indicate the presence of an external capsule that might correspond to the giant protein halomucin, predicted by the genome but never before observed by other microscopy studies. Public Library of Science 2011-04-29 /pmc/articles/PMC3084702/ /pubmed/21559517 http://dx.doi.org/10.1371/journal.pone.0018653 Text en Sublimi Saponetti et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Sublimi Saponetti, Matilde
Bobba, Fabrizio
Salerno, Grazia
Scarfato, Alessandro
Corcelli, Angela
Cucolo, Annamaria
Morphological and Structural Aspects of the Extremely Halophilic Archaeon Haloquadratum walsbyi
title Morphological and Structural Aspects of the Extremely Halophilic Archaeon Haloquadratum walsbyi
title_full Morphological and Structural Aspects of the Extremely Halophilic Archaeon Haloquadratum walsbyi
title_fullStr Morphological and Structural Aspects of the Extremely Halophilic Archaeon Haloquadratum walsbyi
title_full_unstemmed Morphological and Structural Aspects of the Extremely Halophilic Archaeon Haloquadratum walsbyi
title_short Morphological and Structural Aspects of the Extremely Halophilic Archaeon Haloquadratum walsbyi
title_sort morphological and structural aspects of the extremely halophilic archaeon haloquadratum walsbyi
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3084702/
https://www.ncbi.nlm.nih.gov/pubmed/21559517
http://dx.doi.org/10.1371/journal.pone.0018653
work_keys_str_mv AT sublimisaponettimatilde morphologicalandstructuralaspectsoftheextremelyhalophilicarchaeonhaloquadratumwalsbyi
AT bobbafabrizio morphologicalandstructuralaspectsoftheextremelyhalophilicarchaeonhaloquadratumwalsbyi
AT salernograzia morphologicalandstructuralaspectsoftheextremelyhalophilicarchaeonhaloquadratumwalsbyi
AT scarfatoalessandro morphologicalandstructuralaspectsoftheextremelyhalophilicarchaeonhaloquadratumwalsbyi
AT corcelliangela morphologicalandstructuralaspectsoftheextremelyhalophilicarchaeonhaloquadratumwalsbyi
AT cucoloannamaria morphologicalandstructuralaspectsoftheextremelyhalophilicarchaeonhaloquadratumwalsbyi