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Microscopic Optical Projection Tomography In Vivo
We describe a versatile optical projection tomography system for rapid three-dimensional imaging of microscopic specimens in vivo. Our tomographic setup eliminates the in xy and z strongly asymmetric resolution, resulting from optical sectioning in conventional confocal microscopy. It allows for rob...
Autores principales: | , , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3084718/ https://www.ncbi.nlm.nih.gov/pubmed/21559481 http://dx.doi.org/10.1371/journal.pone.0018963 |
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author | Rieckher, Matthias Birk, Udo Jochen Meyer, Heiko Ripoll, Jorge Tavernarakis, Nektarios |
author_facet | Rieckher, Matthias Birk, Udo Jochen Meyer, Heiko Ripoll, Jorge Tavernarakis, Nektarios |
author_sort | Rieckher, Matthias |
collection | PubMed |
description | We describe a versatile optical projection tomography system for rapid three-dimensional imaging of microscopic specimens in vivo. Our tomographic setup eliminates the in xy and z strongly asymmetric resolution, resulting from optical sectioning in conventional confocal microscopy. It allows for robust, high resolution fluorescence as well as absorption imaging of live transparent invertebrate animals such as C. elegans. This system offers considerable advantages over currently available methods when imaging dynamic developmental processes and animal ageing; it permits monitoring of spatio-temporal gene expression and anatomical alterations with single-cell resolution, it utilizes both fluorescence and absorption as a source of contrast, and is easily adaptable for a range of small model organisms. |
format | Text |
id | pubmed-3084718 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-30847182011-05-10 Microscopic Optical Projection Tomography In Vivo Rieckher, Matthias Birk, Udo Jochen Meyer, Heiko Ripoll, Jorge Tavernarakis, Nektarios PLoS One Research Article We describe a versatile optical projection tomography system for rapid three-dimensional imaging of microscopic specimens in vivo. Our tomographic setup eliminates the in xy and z strongly asymmetric resolution, resulting from optical sectioning in conventional confocal microscopy. It allows for robust, high resolution fluorescence as well as absorption imaging of live transparent invertebrate animals such as C. elegans. This system offers considerable advantages over currently available methods when imaging dynamic developmental processes and animal ageing; it permits monitoring of spatio-temporal gene expression and anatomical alterations with single-cell resolution, it utilizes both fluorescence and absorption as a source of contrast, and is easily adaptable for a range of small model organisms. Public Library of Science 2011-04-29 /pmc/articles/PMC3084718/ /pubmed/21559481 http://dx.doi.org/10.1371/journal.pone.0018963 Text en Rieckher et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Rieckher, Matthias Birk, Udo Jochen Meyer, Heiko Ripoll, Jorge Tavernarakis, Nektarios Microscopic Optical Projection Tomography In Vivo |
title | Microscopic Optical Projection Tomography In Vivo
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title_full | Microscopic Optical Projection Tomography In Vivo
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title_fullStr | Microscopic Optical Projection Tomography In Vivo
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title_full_unstemmed | Microscopic Optical Projection Tomography In Vivo
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title_short | Microscopic Optical Projection Tomography In Vivo
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title_sort | microscopic optical projection tomography in vivo |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3084718/ https://www.ncbi.nlm.nih.gov/pubmed/21559481 http://dx.doi.org/10.1371/journal.pone.0018963 |
work_keys_str_mv | AT rieckhermatthias microscopicopticalprojectiontomographyinvivo AT birkudojochen microscopicopticalprojectiontomographyinvivo AT meyerheiko microscopicopticalprojectiontomographyinvivo AT ripolljorge microscopicopticalprojectiontomographyinvivo AT tavernarakisnektarios microscopicopticalprojectiontomographyinvivo |