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Microbiota Modulate Host Gene Expression via MicroRNAs
Microbiota are known to modulate host gene expression, yet the underlying molecular mechanisms remain elusive. MicroRNAs (miRNAs) are importantly implicated in many cellular functions by post-transcriptionally regulating gene expression via binding to the 3′-untranslated regions (3′-UTRs) of the tar...
Autores principales: | , , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3084815/ https://www.ncbi.nlm.nih.gov/pubmed/21559394 http://dx.doi.org/10.1371/journal.pone.0019293 |
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author | Dalmasso, Guillaume Nguyen, Hang Thi Thu Yan, Yutao Laroui, Hamed Charania, Moiz A. Ayyadurai, Saravanan Sitaraman, Shanthi V. Merlin, Didier |
author_facet | Dalmasso, Guillaume Nguyen, Hang Thi Thu Yan, Yutao Laroui, Hamed Charania, Moiz A. Ayyadurai, Saravanan Sitaraman, Shanthi V. Merlin, Didier |
author_sort | Dalmasso, Guillaume |
collection | PubMed |
description | Microbiota are known to modulate host gene expression, yet the underlying molecular mechanisms remain elusive. MicroRNAs (miRNAs) are importantly implicated in many cellular functions by post-transcriptionally regulating gene expression via binding to the 3′-untranslated regions (3′-UTRs) of the target mRNAs. However, a role for miRNAs in microbiota-host interactions remains unknown. Here we investigated if miRNAs are involved in microbiota-mediated regulation of host gene expression. Germ-free mice were colonized with the microbiota from pathogen-free mice. Comparative profiling of miRNA expression using miRNA arrays revealed one and eight miRNAs that were differently expressed in the ileum and the colon, respectively, of colonized mice relative to germ-free mice. A computational approach was then employed to predict genes that were potentially targeted by the dysregulated miRNAs during colonization. Overlapping the miRNA potential targets with the microbiota-induced dysregulated genes detected by a DNA microarray performed in parallel revealed several host genes that were regulated by miRNAs in response to colonization. Among them, Abcc3 was identified as a highly potential miRNA target during colonization. Using the murine macrophage RAW 264.7 cell line, we demonstrated that mmu-miR-665, which was dysregulated during colonization, down-regulated Abcc3 expression by directly targeting the Abcc3 3′-UTR. In conclusion, our study demonstrates that microbiota modulate host microRNA expression, which could in turn regulate host gene expression. |
format | Text |
id | pubmed-3084815 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-30848152011-05-10 Microbiota Modulate Host Gene Expression via MicroRNAs Dalmasso, Guillaume Nguyen, Hang Thi Thu Yan, Yutao Laroui, Hamed Charania, Moiz A. Ayyadurai, Saravanan Sitaraman, Shanthi V. Merlin, Didier PLoS One Research Article Microbiota are known to modulate host gene expression, yet the underlying molecular mechanisms remain elusive. MicroRNAs (miRNAs) are importantly implicated in many cellular functions by post-transcriptionally regulating gene expression via binding to the 3′-untranslated regions (3′-UTRs) of the target mRNAs. However, a role for miRNAs in microbiota-host interactions remains unknown. Here we investigated if miRNAs are involved in microbiota-mediated regulation of host gene expression. Germ-free mice were colonized with the microbiota from pathogen-free mice. Comparative profiling of miRNA expression using miRNA arrays revealed one and eight miRNAs that were differently expressed in the ileum and the colon, respectively, of colonized mice relative to germ-free mice. A computational approach was then employed to predict genes that were potentially targeted by the dysregulated miRNAs during colonization. Overlapping the miRNA potential targets with the microbiota-induced dysregulated genes detected by a DNA microarray performed in parallel revealed several host genes that were regulated by miRNAs in response to colonization. Among them, Abcc3 was identified as a highly potential miRNA target during colonization. Using the murine macrophage RAW 264.7 cell line, we demonstrated that mmu-miR-665, which was dysregulated during colonization, down-regulated Abcc3 expression by directly targeting the Abcc3 3′-UTR. In conclusion, our study demonstrates that microbiota modulate host microRNA expression, which could in turn regulate host gene expression. Public Library of Science 2011-04-29 /pmc/articles/PMC3084815/ /pubmed/21559394 http://dx.doi.org/10.1371/journal.pone.0019293 Text en Dalmasso et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Dalmasso, Guillaume Nguyen, Hang Thi Thu Yan, Yutao Laroui, Hamed Charania, Moiz A. Ayyadurai, Saravanan Sitaraman, Shanthi V. Merlin, Didier Microbiota Modulate Host Gene Expression via MicroRNAs |
title | Microbiota Modulate Host Gene Expression via MicroRNAs |
title_full | Microbiota Modulate Host Gene Expression via MicroRNAs |
title_fullStr | Microbiota Modulate Host Gene Expression via MicroRNAs |
title_full_unstemmed | Microbiota Modulate Host Gene Expression via MicroRNAs |
title_short | Microbiota Modulate Host Gene Expression via MicroRNAs |
title_sort | microbiota modulate host gene expression via micrornas |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3084815/ https://www.ncbi.nlm.nih.gov/pubmed/21559394 http://dx.doi.org/10.1371/journal.pone.0019293 |
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