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Defective Interfering Viral Particles in Acute Dengue Infections
While much of the genetic variation in RNA viruses arises because of the error-prone nature of their RNA-dependent RNA polymerases, much larger changes may occur as a result of recombination. An extreme example of genetic change is found in defective interfering (DI) viral particles, where large sec...
Autores principales: | , , , , , |
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Formato: | Texto |
Lenguaje: | English |
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Public Library of Science
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3084866/ https://www.ncbi.nlm.nih.gov/pubmed/21559384 http://dx.doi.org/10.1371/journal.pone.0019447 |
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author | Li, Dongsheng Lott, William B. Lowry, Kym Jones, Anita Thu, Hlaing Myat Aaskov, John |
author_facet | Li, Dongsheng Lott, William B. Lowry, Kym Jones, Anita Thu, Hlaing Myat Aaskov, John |
author_sort | Li, Dongsheng |
collection | PubMed |
description | While much of the genetic variation in RNA viruses arises because of the error-prone nature of their RNA-dependent RNA polymerases, much larger changes may occur as a result of recombination. An extreme example of genetic change is found in defective interfering (DI) viral particles, where large sections of the genome of a parental virus have been deleted and the residual sub-genome fragment is replicated by complementation by co-infecting functional viruses. While most reports of DI particles have referred to studies in vitro, there is some evidence for the presence of DI particles in chronic viral infections in vivo. In this study, short fragments of dengue virus (DENV) RNA containing only key regulatory elements at the 3′ and 5′ ends of the genome were recovered from the sera of patients infected with any of the four DENV serotypes. Identical RNA fragments were detected in the supernatant from cultures of Aedes mosquito cells that were infected by the addition of sera from dengue patients, suggesting that the sub-genomic RNA might be transmitted between human and mosquito hosts in defective interfering (DI) viral particles. In vitro transcribed sub-genomic RNA corresponding to that detected in vivo could be packaged in virus like particles in the presence of wild type virus and transmitted for at least three passages in cell culture. DENV preparations enriched for these putative DI particles reduced the yield of wild type dengue virus following co-infections of C6–36 cells. This is the first report of DI particles in an acute arboviral infection in nature. The internal genomic deletions described here are the most extensive defects observed in DENV and may be part of a much broader disease attenuating process that is mediated by defective viruses. |
format | Text |
id | pubmed-3084866 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-30848662011-05-10 Defective Interfering Viral Particles in Acute Dengue Infections Li, Dongsheng Lott, William B. Lowry, Kym Jones, Anita Thu, Hlaing Myat Aaskov, John PLoS One Research Article While much of the genetic variation in RNA viruses arises because of the error-prone nature of their RNA-dependent RNA polymerases, much larger changes may occur as a result of recombination. An extreme example of genetic change is found in defective interfering (DI) viral particles, where large sections of the genome of a parental virus have been deleted and the residual sub-genome fragment is replicated by complementation by co-infecting functional viruses. While most reports of DI particles have referred to studies in vitro, there is some evidence for the presence of DI particles in chronic viral infections in vivo. In this study, short fragments of dengue virus (DENV) RNA containing only key regulatory elements at the 3′ and 5′ ends of the genome were recovered from the sera of patients infected with any of the four DENV serotypes. Identical RNA fragments were detected in the supernatant from cultures of Aedes mosquito cells that were infected by the addition of sera from dengue patients, suggesting that the sub-genomic RNA might be transmitted between human and mosquito hosts in defective interfering (DI) viral particles. In vitro transcribed sub-genomic RNA corresponding to that detected in vivo could be packaged in virus like particles in the presence of wild type virus and transmitted for at least three passages in cell culture. DENV preparations enriched for these putative DI particles reduced the yield of wild type dengue virus following co-infections of C6–36 cells. This is the first report of DI particles in an acute arboviral infection in nature. The internal genomic deletions described here are the most extensive defects observed in DENV and may be part of a much broader disease attenuating process that is mediated by defective viruses. Public Library of Science 2011-04-29 /pmc/articles/PMC3084866/ /pubmed/21559384 http://dx.doi.org/10.1371/journal.pone.0019447 Text en Li et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Li, Dongsheng Lott, William B. Lowry, Kym Jones, Anita Thu, Hlaing Myat Aaskov, John Defective Interfering Viral Particles in Acute Dengue Infections |
title | Defective Interfering Viral Particles in Acute Dengue Infections |
title_full | Defective Interfering Viral Particles in Acute Dengue Infections |
title_fullStr | Defective Interfering Viral Particles in Acute Dengue Infections |
title_full_unstemmed | Defective Interfering Viral Particles in Acute Dengue Infections |
title_short | Defective Interfering Viral Particles in Acute Dengue Infections |
title_sort | defective interfering viral particles in acute dengue infections |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3084866/ https://www.ncbi.nlm.nih.gov/pubmed/21559384 http://dx.doi.org/10.1371/journal.pone.0019447 |
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