A Parallel Microfluidic Flow Cytometer for High Content Screening

A parallel microfluidic cytometer (PMC) uses a high-speed scanning photomultiplier-based detector to combine low-pixel-count 1-D imaging with flow cytometry. The 384 parallel flow channels of the PMC decouple count rate from signal-to-noise ratio. Using 6-pixel 1-D images, we investigated protein-lo...

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Detalles Bibliográficos
Autores principales: McKenna, Brian K., Evans, James G., Cheung, Man Ching, Ehrlich, Daniel J.
Formato: Texto
Lenguaje:English
Publicado: 2011
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3084896/
https://www.ncbi.nlm.nih.gov/pubmed/21478861
http://dx.doi.org/10.1038/nmeth.1595
Descripción
Sumario:A parallel microfluidic cytometer (PMC) uses a high-speed scanning photomultiplier-based detector to combine low-pixel-count 1-D imaging with flow cytometry. The 384 parallel flow channels of the PMC decouple count rate from signal-to-noise ratio. Using 6-pixel 1-D images, we investigated protein-localization in a yeast model for a human protein-misfolding disease and demonstrated the feasibility of a nuclear-translocation assay in Chinese-hamster-ovary (CHO) cells expressing a NFκB-GFP reporter.

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