A Parallel Microfluidic Flow Cytometer for High Content Screening

A parallel microfluidic cytometer (PMC) uses a high-speed scanning photomultiplier-based detector to combine low-pixel-count 1-D imaging with flow cytometry. The 384 parallel flow channels of the PMC decouple count rate from signal-to-noise ratio. Using 6-pixel 1-D images, we investigated protein-lo...

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Autores principales: McKenna, Brian K., Evans, James G., Cheung, Man Ching, Ehrlich, Daniel J.
Formato: Texto
Lenguaje:English
Publicado: 2011
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3084896/
https://www.ncbi.nlm.nih.gov/pubmed/21478861
http://dx.doi.org/10.1038/nmeth.1595
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author McKenna, Brian K.
Evans, James G.
Cheung, Man Ching
Ehrlich, Daniel J.
author_facet McKenna, Brian K.
Evans, James G.
Cheung, Man Ching
Ehrlich, Daniel J.
author_sort McKenna, Brian K.
collection PubMed
description A parallel microfluidic cytometer (PMC) uses a high-speed scanning photomultiplier-based detector to combine low-pixel-count 1-D imaging with flow cytometry. The 384 parallel flow channels of the PMC decouple count rate from signal-to-noise ratio. Using 6-pixel 1-D images, we investigated protein-localization in a yeast model for a human protein-misfolding disease and demonstrated the feasibility of a nuclear-translocation assay in Chinese-hamster-ovary (CHO) cells expressing a NFκB-GFP reporter.
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spelling pubmed-30848962011-11-01 A Parallel Microfluidic Flow Cytometer for High Content Screening McKenna, Brian K. Evans, James G. Cheung, Man Ching Ehrlich, Daniel J. Nat Methods Article A parallel microfluidic cytometer (PMC) uses a high-speed scanning photomultiplier-based detector to combine low-pixel-count 1-D imaging with flow cytometry. The 384 parallel flow channels of the PMC decouple count rate from signal-to-noise ratio. Using 6-pixel 1-D images, we investigated protein-localization in a yeast model for a human protein-misfolding disease and demonstrated the feasibility of a nuclear-translocation assay in Chinese-hamster-ovary (CHO) cells expressing a NFκB-GFP reporter. 2011-04-10 2011-05 /pmc/articles/PMC3084896/ /pubmed/21478861 http://dx.doi.org/10.1038/nmeth.1595 Text en Users may view, print, copy, download and text and data- mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use: http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
McKenna, Brian K.
Evans, James G.
Cheung, Man Ching
Ehrlich, Daniel J.
A Parallel Microfluidic Flow Cytometer for High Content Screening
title A Parallel Microfluidic Flow Cytometer for High Content Screening
title_full A Parallel Microfluidic Flow Cytometer for High Content Screening
title_fullStr A Parallel Microfluidic Flow Cytometer for High Content Screening
title_full_unstemmed A Parallel Microfluidic Flow Cytometer for High Content Screening
title_short A Parallel Microfluidic Flow Cytometer for High Content Screening
title_sort parallel microfluidic flow cytometer for high content screening
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3084896/
https://www.ncbi.nlm.nih.gov/pubmed/21478861
http://dx.doi.org/10.1038/nmeth.1595
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