Optimization of Inulinase Production from Garlic by Streptomyces sp. in Solid State Fermentation Using Statistical Designs

Plackett-Burman design was employed for screening 18 nutrient components for the production of inulinase using Garlic as substrate by Streptomyces sp. in solid-state fermentation (SSF). From the experiments, 4 nutrients, namely, NH(4)NO(3), MnSO(4)·7H(2)O, Soya bean cake, and K(2)HPO(4) were found t...

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Detalles Bibliográficos
Autores principales: Dilipkumar, M., Rajasimman, M., Rajamohan, N.
Formato: Texto
Lenguaje:English
Publicado: SAGE-Hindawi Access to Research 2011
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3085327/
https://www.ncbi.nlm.nih.gov/pubmed/21541216
http://dx.doi.org/10.4061/2011/708043
Descripción
Sumario:Plackett-Burman design was employed for screening 18 nutrient components for the production of inulinase using Garlic as substrate by Streptomyces sp. in solid-state fermentation (SSF). From the experiments, 4 nutrients, namely, NH(4)NO(3), MnSO(4)·7H(2)O, Soya bean cake, and K(2)HPO(4) were found to be most significant nutrient components. Hence, these 4 components are selected. The selected components were optimized using response surface methodology (RSM). The optimum conditions are NH(4)NO(3)—6.63 mg/gds, MnSO(4)·7H(2)O—26.16 mg/gds, Soya bean cake—60.6 mg/gds, and K(2)HPO(4)—5.24 mg/gds. Under these conditions, the production of inulinase was found to be 76 U/gds.

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