Optimization of Inulinase Production from Garlic by Streptomyces sp. in Solid State Fermentation Using Statistical Designs

Plackett-Burman design was employed for screening 18 nutrient components for the production of inulinase using Garlic as substrate by Streptomyces sp. in solid-state fermentation (SSF). From the experiments, 4 nutrients, namely, NH(4)NO(3), MnSO(4)·7H(2)O, Soya bean cake, and K(2)HPO(4) were found t...

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Autores principales: Dilipkumar, M., Rajasimman, M., Rajamohan, N.
Formato: Texto
Lenguaje:English
Publicado: SAGE-Hindawi Access to Research 2011
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3085327/
https://www.ncbi.nlm.nih.gov/pubmed/21541216
http://dx.doi.org/10.4061/2011/708043
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author Dilipkumar, M.
Rajasimman, M.
Rajamohan, N.
author_facet Dilipkumar, M.
Rajasimman, M.
Rajamohan, N.
author_sort Dilipkumar, M.
collection PubMed
description Plackett-Burman design was employed for screening 18 nutrient components for the production of inulinase using Garlic as substrate by Streptomyces sp. in solid-state fermentation (SSF). From the experiments, 4 nutrients, namely, NH(4)NO(3), MnSO(4)·7H(2)O, Soya bean cake, and K(2)HPO(4) were found to be most significant nutrient components. Hence, these 4 components are selected. The selected components were optimized using response surface methodology (RSM). The optimum conditions are NH(4)NO(3)—6.63 mg/gds, MnSO(4)·7H(2)O—26.16 mg/gds, Soya bean cake—60.6 mg/gds, and K(2)HPO(4)—5.24 mg/gds. Under these conditions, the production of inulinase was found to be 76 U/gds.
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spelling pubmed-30853272011-05-03 Optimization of Inulinase Production from Garlic by Streptomyces sp. in Solid State Fermentation Using Statistical Designs Dilipkumar, M. Rajasimman, M. Rajamohan, N. Biotechnol Res Int Research Article Plackett-Burman design was employed for screening 18 nutrient components for the production of inulinase using Garlic as substrate by Streptomyces sp. in solid-state fermentation (SSF). From the experiments, 4 nutrients, namely, NH(4)NO(3), MnSO(4)·7H(2)O, Soya bean cake, and K(2)HPO(4) were found to be most significant nutrient components. Hence, these 4 components are selected. The selected components were optimized using response surface methodology (RSM). The optimum conditions are NH(4)NO(3)—6.63 mg/gds, MnSO(4)·7H(2)O—26.16 mg/gds, Soya bean cake—60.6 mg/gds, and K(2)HPO(4)—5.24 mg/gds. Under these conditions, the production of inulinase was found to be 76 U/gds. SAGE-Hindawi Access to Research 2011-03-30 /pmc/articles/PMC3085327/ /pubmed/21541216 http://dx.doi.org/10.4061/2011/708043 Text en Copyright © 2011 M. Dilipkumar et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Dilipkumar, M.
Rajasimman, M.
Rajamohan, N.
Optimization of Inulinase Production from Garlic by Streptomyces sp. in Solid State Fermentation Using Statistical Designs
title Optimization of Inulinase Production from Garlic by Streptomyces sp. in Solid State Fermentation Using Statistical Designs
title_full Optimization of Inulinase Production from Garlic by Streptomyces sp. in Solid State Fermentation Using Statistical Designs
title_fullStr Optimization of Inulinase Production from Garlic by Streptomyces sp. in Solid State Fermentation Using Statistical Designs
title_full_unstemmed Optimization of Inulinase Production from Garlic by Streptomyces sp. in Solid State Fermentation Using Statistical Designs
title_short Optimization of Inulinase Production from Garlic by Streptomyces sp. in Solid State Fermentation Using Statistical Designs
title_sort optimization of inulinase production from garlic by streptomyces sp. in solid state fermentation using statistical designs
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3085327/
https://www.ncbi.nlm.nih.gov/pubmed/21541216
http://dx.doi.org/10.4061/2011/708043
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