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Effects of the NO donor sodium nitroprusside on oxygen consumption and energetics in rabbit myocardium
Nitric oxide (NO) has influence on various cellular functions. Little is known of the influence of NO on myocardial energetics. In the present study oxygen consumption and mechanical parameters of isometrically contracting rabbit papillary muscles (1 Hz stimulation frequency) were investigated at va...
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Formato: | Texto |
Lenguaje: | English |
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Steinkopff-Verlag
2009
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3085761/ https://www.ncbi.nlm.nih.gov/pubmed/19190952 http://dx.doi.org/10.1007/s00395-009-0777-9 |
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author | Hünlich, Mark Hasenfuss, Gerd |
author_facet | Hünlich, Mark Hasenfuss, Gerd |
author_sort | Hünlich, Mark |
collection | PubMed |
description | Nitric oxide (NO) has influence on various cellular functions. Little is known of the influence of NO on myocardial energetics. In the present study oxygen consumption and mechanical parameters of isometrically contracting rabbit papillary muscles (1 Hz stimulation frequency) were investigated at varying interventions while maintaining physiological conditions (37°C; 2.5 mM Ca(2+)) to study the effects of NO on energetics. The NO donor sodium nitroprusside (SNP) showed a negative inotropic effect. SNP decreased the maximal force in normal rabbit muscle strips by 30%, the force time integral (FTI) by 40% and the relaxation time by 20%. In addition the oxygen consumption decreased by 60%, a notably disproportional decrease compared to the mechanical parameters. Consequently, the economy as a ratio of FTI and oxygen consumption is significantly increased by SNP. In contrast the negative inotropic effect due to a reduction in extracellular Calcium (Ca(2+)) from 2.5 to 1.25 mM reduced FTI and oxygen consumption proportionally by 40% and did not change economy. The effect of NO on force and oxygen consumption could be reproduced by the application of the cyclic guanosine monophosphate (cGMP) analogue 8-bromo-cGMP. In summary, NO increased the economy of isometrically contracting papillary muscles. The improvement in contraction economy under NO seems to be mediated by cGMP as the secondary messenger and maybe due to alterations of the crossbridge cycle. |
format | Text |
id | pubmed-3085761 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2009 |
publisher | Steinkopff-Verlag |
record_format | MEDLINE/PubMed |
spelling | pubmed-30857612011-06-06 Effects of the NO donor sodium nitroprusside on oxygen consumption and energetics in rabbit myocardium Hünlich, Mark Hasenfuss, Gerd Basic Res Cardiol Original Contribution Nitric oxide (NO) has influence on various cellular functions. Little is known of the influence of NO on myocardial energetics. In the present study oxygen consumption and mechanical parameters of isometrically contracting rabbit papillary muscles (1 Hz stimulation frequency) were investigated at varying interventions while maintaining physiological conditions (37°C; 2.5 mM Ca(2+)) to study the effects of NO on energetics. The NO donor sodium nitroprusside (SNP) showed a negative inotropic effect. SNP decreased the maximal force in normal rabbit muscle strips by 30%, the force time integral (FTI) by 40% and the relaxation time by 20%. In addition the oxygen consumption decreased by 60%, a notably disproportional decrease compared to the mechanical parameters. Consequently, the economy as a ratio of FTI and oxygen consumption is significantly increased by SNP. In contrast the negative inotropic effect due to a reduction in extracellular Calcium (Ca(2+)) from 2.5 to 1.25 mM reduced FTI and oxygen consumption proportionally by 40% and did not change economy. The effect of NO on force and oxygen consumption could be reproduced by the application of the cyclic guanosine monophosphate (cGMP) analogue 8-bromo-cGMP. In summary, NO increased the economy of isometrically contracting papillary muscles. The improvement in contraction economy under NO seems to be mediated by cGMP as the secondary messenger and maybe due to alterations of the crossbridge cycle. Steinkopff-Verlag 2009-02-03 2009-07 /pmc/articles/PMC3085761/ /pubmed/19190952 http://dx.doi.org/10.1007/s00395-009-0777-9 Text en © Steinkopff Verlag Darmstadt 2009 |
spellingShingle | Original Contribution Hünlich, Mark Hasenfuss, Gerd Effects of the NO donor sodium nitroprusside on oxygen consumption and energetics in rabbit myocardium |
title | Effects of the NO donor sodium nitroprusside on oxygen consumption and energetics in rabbit myocardium |
title_full | Effects of the NO donor sodium nitroprusside on oxygen consumption and energetics in rabbit myocardium |
title_fullStr | Effects of the NO donor sodium nitroprusside on oxygen consumption and energetics in rabbit myocardium |
title_full_unstemmed | Effects of the NO donor sodium nitroprusside on oxygen consumption and energetics in rabbit myocardium |
title_short | Effects of the NO donor sodium nitroprusside on oxygen consumption and energetics in rabbit myocardium |
title_sort | effects of the no donor sodium nitroprusside on oxygen consumption and energetics in rabbit myocardium |
topic | Original Contribution |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3085761/ https://www.ncbi.nlm.nih.gov/pubmed/19190952 http://dx.doi.org/10.1007/s00395-009-0777-9 |
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