The solution structure of coronaviral stem‐loop 2 (SL2) reveals a canonical CUYG tetraloop fold

The transcription and replication of the severe acute respiratory syndrome (SARS) coronavirus (SARS‐CoV) is regulated by specific viral genome sequences within 5′‐ and 3′‐untranslated regions (5′‐UTR and 3′‐UTR). Here we report the solution structure of 5′‐UTR derived stem‐loop 2 (SL2) of SARS‐CoV d...

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Detalles Bibliográficos
Autores principales: Lee, Chul Won, Li, Lichun, Giedroc, David P.
Formato: Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2011
Materias:
Short Communications
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3086565/
https://www.ncbi.nlm.nih.gov/pubmed/21382373
http://dx.doi.org/10.1016/j.febslet.2011.03.002
Descripción
Sumario:The transcription and replication of the severe acute respiratory syndrome (SARS) coronavirus (SARS‐CoV) is regulated by specific viral genome sequences within 5′‐ and 3′‐untranslated regions (5′‐UTR and 3′‐UTR). Here we report the solution structure of 5′‐UTR derived stem‐loop 2 (SL2) of SARS‐CoV determined by NMR spectroscopy. The highly conserved pentaloop of SL2 is stacked on 5‐bp stem and adopts a canonical CUYG tetraloop fold with the 3′ nucleotide (U51) flipped out of the stack. The significance of this structure in the context of a previous mutagenesis analysis of SL2 function in replication of the related group 2 coronavirus, mouse hepatitis virus, is discussed.

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