The solution structure of coronaviral stem‐loop 2 (SL2) reveals a canonical CUYG tetraloop fold

The transcription and replication of the severe acute respiratory syndrome (SARS) coronavirus (SARS‐CoV) is regulated by specific viral genome sequences within 5′‐ and 3′‐untranslated regions (5′‐UTR and 3′‐UTR). Here we report the solution structure of 5′‐UTR derived stem‐loop 2 (SL2) of SARS‐CoV d...

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Detalles Bibliográficos
Autores principales: Lee, Chul Won, Li, Lichun, Giedroc, David P.
Formato: Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2011
Materias:
Short Communications
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3086565/
https://www.ncbi.nlm.nih.gov/pubmed/21382373
http://dx.doi.org/10.1016/j.febslet.2011.03.002
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author Lee, Chul Won
Li, Lichun
Giedroc, David P.
author_facet Lee, Chul Won
Li, Lichun
Giedroc, David P.
author_sort Lee, Chul Won
collection PubMed
description The transcription and replication of the severe acute respiratory syndrome (SARS) coronavirus (SARS‐CoV) is regulated by specific viral genome sequences within 5′‐ and 3′‐untranslated regions (5′‐UTR and 3′‐UTR). Here we report the solution structure of 5′‐UTR derived stem‐loop 2 (SL2) of SARS‐CoV determined by NMR spectroscopy. The highly conserved pentaloop of SL2 is stacked on 5‐bp stem and adopts a canonical CUYG tetraloop fold with the 3′ nucleotide (U51) flipped out of the stack. The significance of this structure in the context of a previous mutagenesis analysis of SL2 function in replication of the related group 2 coronavirus, mouse hepatitis virus, is discussed.
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spelling pubmed-30865652012-04-06 The solution structure of coronaviral stem‐loop 2 (SL2) reveals a canonical CUYG tetraloop fold Lee, Chul Won Li, Lichun Giedroc, David P. FEBS Lett Short Communications The transcription and replication of the severe acute respiratory syndrome (SARS) coronavirus (SARS‐CoV) is regulated by specific viral genome sequences within 5′‐ and 3′‐untranslated regions (5′‐UTR and 3′‐UTR). Here we report the solution structure of 5′‐UTR derived stem‐loop 2 (SL2) of SARS‐CoV determined by NMR spectroscopy. The highly conserved pentaloop of SL2 is stacked on 5‐bp stem and adopts a canonical CUYG tetraloop fold with the 3′ nucleotide (U51) flipped out of the stack. The significance of this structure in the context of a previous mutagenesis analysis of SL2 function in replication of the related group 2 coronavirus, mouse hepatitis virus, is discussed. John Wiley and Sons Inc. 2011-04-06 2011-03-04 /pmc/articles/PMC3086565/ /pubmed/21382373 http://dx.doi.org/10.1016/j.febslet.2011.03.002 Text en FEBS Letters 585 (2011) 1873-3468 © 2015 Federation of European Biochemical Societies This article is being made freely available through PubMed Central as part of the COVID-19 public health emergency response. It can be used for unrestricted research re-use and analysis in any form or by any means with acknowledgement of the original source, for the duration of the public health emergency.
spellingShingle Short Communications
Lee, Chul Won
Li, Lichun
Giedroc, David P.
The solution structure of coronaviral stem‐loop 2 (SL2) reveals a canonical CUYG tetraloop fold
title The solution structure of coronaviral stem‐loop 2 (SL2) reveals a canonical CUYG tetraloop fold
title_full The solution structure of coronaviral stem‐loop 2 (SL2) reveals a canonical CUYG tetraloop fold
title_fullStr The solution structure of coronaviral stem‐loop 2 (SL2) reveals a canonical CUYG tetraloop fold
title_full_unstemmed The solution structure of coronaviral stem‐loop 2 (SL2) reveals a canonical CUYG tetraloop fold
title_short The solution structure of coronaviral stem‐loop 2 (SL2) reveals a canonical CUYG tetraloop fold
title_sort solution structure of coronaviral stem‐loop 2 (sl2) reveals a canonical cuyg tetraloop fold
topic Short Communications
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3086565/
https://www.ncbi.nlm.nih.gov/pubmed/21382373
http://dx.doi.org/10.1016/j.febslet.2011.03.002
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