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A mechanism for extremely weak SpaP-expression in Streptococcus mutans strain Z1
BACKGROUND: Streptococcus mutans surface-protein antigen (SpaP, PAc, or antigen I/II) has been well known to play an important role in initial attachment to tooth surfaces. However, strains with weak SpaP-expression were recently reported to be found in natural populations of S. mutans. The S. mutan...
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Formato: | Texto |
Lenguaje: | English |
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CoAction Publishing
2011
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3086597/ https://www.ncbi.nlm.nih.gov/pubmed/21541094 http://dx.doi.org/10.3402/jom.v3i0.5495 |
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author | Sato, Yutaka Okamoto-Shibayama, Kazuko Azuma, Toshifumi |
author_facet | Sato, Yutaka Okamoto-Shibayama, Kazuko Azuma, Toshifumi |
author_sort | Sato, Yutaka |
collection | PubMed |
description | BACKGROUND: Streptococcus mutans surface-protein antigen (SpaP, PAc, or antigen I/II) has been well known to play an important role in initial attachment to tooth surfaces. However, strains with weak SpaP-expression were recently reported to be found in natural populations of S. mutans. The S. mutans gbpC-negative strain Z1, which we previously isolated from saliva and plaque samples, apparently expresses relatively low levels of SpaP protein compared to S. mutans strains MT8148 or UA159. OBJECTIVE: To elucidate the mechanism for weak SpaP-expression in this strain, the spaP gene region in strain Z1 was amplified by polymerase chain reaction (PCR) and analyzed. METHODS: Allelic exchange mutants between strains Z1 and UA159 involving the spaP gene region were constructed. The SpaP protein expressed in the mutants was detected with Coomasie Brilliant Blue (CBB)-staining and Western blot analysis following SDS-PAGE. RESULTS: The 4689 bp spaP gene coding sequence for Z1 appeared to be intact. In contrast, a 20 bp nucleotide sequence appeared to be deleted from the region immediately upstream from the Z1 spaP gene when compared to the same region in UA159. The 216 bp and 237 bp intergenic fragments upstream from the spaP gene, respectively, from Z1 and UA159 were isolated, modified, and transformed into the other strain by allelic replacement. The resultant UA159-promoter region-mutant exhibited extremely weak SpaP-expression similar to that of strain Z1 and the Z1 complemented mutant expressed Spa protein levels like that of strain UA159. CONCLUSION: These results suggest that weak SpaP-expression in strain Z1 resulted from a 20 bp-deletion in the spaP gene promoter region. |
format | Text |
id | pubmed-3086597 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | CoAction Publishing |
record_format | MEDLINE/PubMed |
spelling | pubmed-30865972011-05-03 A mechanism for extremely weak SpaP-expression in Streptococcus mutans strain Z1 Sato, Yutaka Okamoto-Shibayama, Kazuko Azuma, Toshifumi J Oral Microbiol Short Communications BACKGROUND: Streptococcus mutans surface-protein antigen (SpaP, PAc, or antigen I/II) has been well known to play an important role in initial attachment to tooth surfaces. However, strains with weak SpaP-expression were recently reported to be found in natural populations of S. mutans. The S. mutans gbpC-negative strain Z1, which we previously isolated from saliva and plaque samples, apparently expresses relatively low levels of SpaP protein compared to S. mutans strains MT8148 or UA159. OBJECTIVE: To elucidate the mechanism for weak SpaP-expression in this strain, the spaP gene region in strain Z1 was amplified by polymerase chain reaction (PCR) and analyzed. METHODS: Allelic exchange mutants between strains Z1 and UA159 involving the spaP gene region were constructed. The SpaP protein expressed in the mutants was detected with Coomasie Brilliant Blue (CBB)-staining and Western blot analysis following SDS-PAGE. RESULTS: The 4689 bp spaP gene coding sequence for Z1 appeared to be intact. In contrast, a 20 bp nucleotide sequence appeared to be deleted from the region immediately upstream from the Z1 spaP gene when compared to the same region in UA159. The 216 bp and 237 bp intergenic fragments upstream from the spaP gene, respectively, from Z1 and UA159 were isolated, modified, and transformed into the other strain by allelic replacement. The resultant UA159-promoter region-mutant exhibited extremely weak SpaP-expression similar to that of strain Z1 and the Z1 complemented mutant expressed Spa protein levels like that of strain UA159. CONCLUSION: These results suggest that weak SpaP-expression in strain Z1 resulted from a 20 bp-deletion in the spaP gene promoter region. CoAction Publishing 2011-04-14 /pmc/articles/PMC3086597/ /pubmed/21541094 http://dx.doi.org/10.3402/jom.v3i0.5495 Text en © 2011 Yutaka Sato et al. http://creativecommons.org/licenses/by-nc/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution-Noncommercial 3.0 Unported License, permitting all non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Short Communications Sato, Yutaka Okamoto-Shibayama, Kazuko Azuma, Toshifumi A mechanism for extremely weak SpaP-expression in Streptococcus mutans strain Z1 |
title | A mechanism for extremely weak SpaP-expression in Streptococcus mutans strain Z1 |
title_full | A mechanism for extremely weak SpaP-expression in Streptococcus mutans strain Z1 |
title_fullStr | A mechanism for extremely weak SpaP-expression in Streptococcus mutans strain Z1 |
title_full_unstemmed | A mechanism for extremely weak SpaP-expression in Streptococcus mutans strain Z1 |
title_short | A mechanism for extremely weak SpaP-expression in Streptococcus mutans strain Z1 |
title_sort | mechanism for extremely weak spap-expression in streptococcus mutans strain z1 |
topic | Short Communications |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3086597/ https://www.ncbi.nlm.nih.gov/pubmed/21541094 http://dx.doi.org/10.3402/jom.v3i0.5495 |
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