Cargando…
Cell wound repair in Drosophila occurs through three distinct phases of membrane and cytoskeletal remodeling
When single cells or tissues are injured, the wound must be repaired quickly in order to prevent cell death, loss of tissue integrity, and invasion by microorganisms. We describe Drosophila as a genetically tractable model to dissect the mechanisms of single-cell wound repair. By analyzing the expre...
Autores principales: | , , |
---|---|
Formato: | Texto |
Lenguaje: | English |
Publicado: |
The Rockefeller University Press
2011
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3087011/ https://www.ncbi.nlm.nih.gov/pubmed/21518790 http://dx.doi.org/10.1083/jcb.201011018 |
_version_ | 1782202730770595840 |
---|---|
author | Abreu-Blanco, Maria Teresa Verboon, Jeffrey M. Parkhurst, Susan M. |
author_facet | Abreu-Blanco, Maria Teresa Verboon, Jeffrey M. Parkhurst, Susan M. |
author_sort | Abreu-Blanco, Maria Teresa |
collection | PubMed |
description | When single cells or tissues are injured, the wound must be repaired quickly in order to prevent cell death, loss of tissue integrity, and invasion by microorganisms. We describe Drosophila as a genetically tractable model to dissect the mechanisms of single-cell wound repair. By analyzing the expression and the effects of perturbations of actin, myosin, microtubules, E-cadherin, and the plasma membrane, we define three distinct phases in the repair process—expansion, contraction, and closure—and identify specific components required during each phase. Specifically, plasma membrane mobilization and assembly of a contractile actomyosin ring are required for this process. In addition, E-cadherin accumulates at the wound edge, and wound expansion is excessive in E-cadherin mutants, suggesting a role for E-cadherin in anchoring the actomyosin ring to the plasma membrane. Our results show that single-cell wound repair requires specific spatial and temporal cytoskeleton responses with distinct components and mechanisms required at different stages of the process. |
format | Text |
id | pubmed-3087011 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-30870112011-11-02 Cell wound repair in Drosophila occurs through three distinct phases of membrane and cytoskeletal remodeling Abreu-Blanco, Maria Teresa Verboon, Jeffrey M. Parkhurst, Susan M. J Cell Biol Research Articles When single cells or tissues are injured, the wound must be repaired quickly in order to prevent cell death, loss of tissue integrity, and invasion by microorganisms. We describe Drosophila as a genetically tractable model to dissect the mechanisms of single-cell wound repair. By analyzing the expression and the effects of perturbations of actin, myosin, microtubules, E-cadherin, and the plasma membrane, we define three distinct phases in the repair process—expansion, contraction, and closure—and identify specific components required during each phase. Specifically, plasma membrane mobilization and assembly of a contractile actomyosin ring are required for this process. In addition, E-cadherin accumulates at the wound edge, and wound expansion is excessive in E-cadherin mutants, suggesting a role for E-cadherin in anchoring the actomyosin ring to the plasma membrane. Our results show that single-cell wound repair requires specific spatial and temporal cytoskeleton responses with distinct components and mechanisms required at different stages of the process. The Rockefeller University Press 2011-05-02 /pmc/articles/PMC3087011/ /pubmed/21518790 http://dx.doi.org/10.1083/jcb.201011018 Text en © 2011 Abreu-Blanco et al. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/). |
spellingShingle | Research Articles Abreu-Blanco, Maria Teresa Verboon, Jeffrey M. Parkhurst, Susan M. Cell wound repair in Drosophila occurs through three distinct phases of membrane and cytoskeletal remodeling |
title | Cell wound repair in Drosophila occurs through three distinct phases of membrane and cytoskeletal remodeling |
title_full | Cell wound repair in Drosophila occurs through three distinct phases of membrane and cytoskeletal remodeling |
title_fullStr | Cell wound repair in Drosophila occurs through three distinct phases of membrane and cytoskeletal remodeling |
title_full_unstemmed | Cell wound repair in Drosophila occurs through three distinct phases of membrane and cytoskeletal remodeling |
title_short | Cell wound repair in Drosophila occurs through three distinct phases of membrane and cytoskeletal remodeling |
title_sort | cell wound repair in drosophila occurs through three distinct phases of membrane and cytoskeletal remodeling |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3087011/ https://www.ncbi.nlm.nih.gov/pubmed/21518790 http://dx.doi.org/10.1083/jcb.201011018 |
work_keys_str_mv | AT abreublancomariateresa cellwoundrepairindrosophilaoccursthroughthreedistinctphasesofmembraneandcytoskeletalremodeling AT verboonjeffreym cellwoundrepairindrosophilaoccursthroughthreedistinctphasesofmembraneandcytoskeletalremodeling AT parkhurstsusanm cellwoundrepairindrosophilaoccursthroughthreedistinctphasesofmembraneandcytoskeletalremodeling |