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Dual-channel imaging system for singlet oxygen and photosensitizer for PDT
A two-channel optical system has been developed to provide spatially resolved simultaneous imaging of singlet molecular oxygen ((1)O(2)) phosphorescence and photosensitizer (PS) fluorescence produced by the photodynamic process. The current imaging system uses a spectral discrimination method to dif...
Autores principales: | , , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
Optical Society of America
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3087579/ https://www.ncbi.nlm.nih.gov/pubmed/21559134 http://dx.doi.org/10.1364/BOE.2.001233 |
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author | Lee, Seonkyung Isabelle, Martin E. Gabally-Kinney, Kristin L. Pogue, Brian W. Davis, Steven J. |
author_facet | Lee, Seonkyung Isabelle, Martin E. Gabally-Kinney, Kristin L. Pogue, Brian W. Davis, Steven J. |
author_sort | Lee, Seonkyung |
collection | PubMed |
description | A two-channel optical system has been developed to provide spatially resolved simultaneous imaging of singlet molecular oxygen ((1)O(2)) phosphorescence and photosensitizer (PS) fluorescence produced by the photodynamic process. The current imaging system uses a spectral discrimination method to differentiate the weak (1)O(2) phosphorescence that peaks near 1.27 μm from PS fluorescence that also occurs in this spectral region. The detection limit of (1)O(2) emission was determined at a concentration of 500 nM benzoporphyrin derivative monoacid (BPD) in tissue-like phantoms, and these signals observed were proportional to the PS fluorescence. Preliminary in vivo images with tumor laden mice indicate that it is possible to obtain simultaneous images of (1)O(2) and PS tissue distribution. |
format | Text |
id | pubmed-3087579 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | Optical Society of America |
record_format | MEDLINE/PubMed |
spelling | pubmed-30875792011-05-10 Dual-channel imaging system for singlet oxygen and photosensitizer for PDT Lee, Seonkyung Isabelle, Martin E. Gabally-Kinney, Kristin L. Pogue, Brian W. Davis, Steven J. Biomed Opt Express Optical Therapies and Photomodificaton A two-channel optical system has been developed to provide spatially resolved simultaneous imaging of singlet molecular oxygen ((1)O(2)) phosphorescence and photosensitizer (PS) fluorescence produced by the photodynamic process. The current imaging system uses a spectral discrimination method to differentiate the weak (1)O(2) phosphorescence that peaks near 1.27 μm from PS fluorescence that also occurs in this spectral region. The detection limit of (1)O(2) emission was determined at a concentration of 500 nM benzoporphyrin derivative monoacid (BPD) in tissue-like phantoms, and these signals observed were proportional to the PS fluorescence. Preliminary in vivo images with tumor laden mice indicate that it is possible to obtain simultaneous images of (1)O(2) and PS tissue distribution. Optical Society of America 2011-04-15 /pmc/articles/PMC3087579/ /pubmed/21559134 http://dx.doi.org/10.1364/BOE.2.001233 Text en ©2011 Optical Society of America http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-No Derivative Works 3.0 Unported License, which permits download and redistribution, provided that the original work is properly cited. This license restricts the article from being modified or used commercially. |
spellingShingle | Optical Therapies and Photomodificaton Lee, Seonkyung Isabelle, Martin E. Gabally-Kinney, Kristin L. Pogue, Brian W. Davis, Steven J. Dual-channel imaging system for singlet oxygen and photosensitizer for PDT |
title | Dual-channel imaging system for singlet oxygen and photosensitizer for PDT |
title_full | Dual-channel imaging system for singlet oxygen and photosensitizer for PDT |
title_fullStr | Dual-channel imaging system for singlet oxygen and photosensitizer for PDT |
title_full_unstemmed | Dual-channel imaging system for singlet oxygen and photosensitizer for PDT |
title_short | Dual-channel imaging system for singlet oxygen and photosensitizer for PDT |
title_sort | dual-channel imaging system for singlet oxygen and photosensitizer for pdt |
topic | Optical Therapies and Photomodificaton |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3087579/ https://www.ncbi.nlm.nih.gov/pubmed/21559134 http://dx.doi.org/10.1364/BOE.2.001233 |
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