In vivo fluorescence lifetime optical projection tomography

We demonstrate the application of fluorescence lifetime optical projection tomography (FLIM-OPT) to in vivo imaging of lysC:GFP transgenic zebrafish embryos (Danio rerio). This method has been applied to unambiguously distinguish between the fluorescent protein (GFP) signal in myeloid cells from bac...

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Detalles Bibliográficos
Autores principales: McGinty, James, Taylor, Harriet B., Chen, Lingling, Bugeon, Laurence, Lamb, Jonathan R., Dallman, Margaret J., French, Paul M. W.
Formato: Texto
Lenguaje:English
Publicado: Optical Society of America 2011
Materias:
Microscopy
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3087590/
https://www.ncbi.nlm.nih.gov/pubmed/21559145
http://dx.doi.org/10.1364/BOE.2.001340
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author McGinty, James
Taylor, Harriet B.
Chen, Lingling
Bugeon, Laurence
Lamb, Jonathan R.
Dallman, Margaret J.
French, Paul M. W.
author_facet McGinty, James
Taylor, Harriet B.
Chen, Lingling
Bugeon, Laurence
Lamb, Jonathan R.
Dallman, Margaret J.
French, Paul M. W.
author_sort McGinty, James
collection PubMed
description We demonstrate the application of fluorescence lifetime optical projection tomography (FLIM-OPT) to in vivo imaging of lysC:GFP transgenic zebrafish embryos (Danio rerio). This method has been applied to unambiguously distinguish between the fluorescent protein (GFP) signal in myeloid cells from background autofluorescence based on the fluorescence lifetime. The combination of FLIM, an inherently ratiometric method, in conjunction with OPT results in a quantitative 3-D tomographic technique that could be used as a robust method for in vivo biological and pharmaceutical research, for example as a readout of Förster resonance energy transfer based interactions.
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spelling pubmed-30875902011-05-10 In vivo fluorescence lifetime optical projection tomography McGinty, James Taylor, Harriet B. Chen, Lingling Bugeon, Laurence Lamb, Jonathan R. Dallman, Margaret J. French, Paul M. W. Biomed Opt Express Microscopy We demonstrate the application of fluorescence lifetime optical projection tomography (FLIM-OPT) to in vivo imaging of lysC:GFP transgenic zebrafish embryos (Danio rerio). This method has been applied to unambiguously distinguish between the fluorescent protein (GFP) signal in myeloid cells from background autofluorescence based on the fluorescence lifetime. The combination of FLIM, an inherently ratiometric method, in conjunction with OPT results in a quantitative 3-D tomographic technique that could be used as a robust method for in vivo biological and pharmaceutical research, for example as a readout of Förster resonance energy transfer based interactions. Optical Society of America 2011-04-26 /pmc/articles/PMC3087590/ /pubmed/21559145 http://dx.doi.org/10.1364/BOE.2.001340 Text en ©2011 Optical Society of America http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-No Derivative Works 3.0 Unported License, which permits download and redistribution, provided that the original work is properly cited. This license restricts the article from being modified or used commercially.
spellingShingle Microscopy
McGinty, James
Taylor, Harriet B.
Chen, Lingling
Bugeon, Laurence
Lamb, Jonathan R.
Dallman, Margaret J.
French, Paul M. W.
In vivo fluorescence lifetime optical projection tomography
title In vivo fluorescence lifetime optical projection tomography
title_full In vivo fluorescence lifetime optical projection tomography
title_fullStr In vivo fluorescence lifetime optical projection tomography
title_full_unstemmed In vivo fluorescence lifetime optical projection tomography
title_short In vivo fluorescence lifetime optical projection tomography
title_sort in vivo fluorescence lifetime optical projection tomography
topic Microscopy
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3087590/
https://www.ncbi.nlm.nih.gov/pubmed/21559145
http://dx.doi.org/10.1364/BOE.2.001340
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AT dallmanmargaretj invivofluorescencelifetimeopticalprojectiontomography
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